| Project/Area Number |
18K07771
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 52040:Radiological sciences-related
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
深田 淳一 慶應義塾大学, 医学部(信濃町), 講師 (50338159)
公田 龍一 慶應義塾大学, 医学部(信濃町), 助教 (00464834)
小池 直義 慶應義塾大学, 医学部(信濃町), 助教 (60464913)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 放射線生物学 / 膵癌 / 放射線抵抗性 / オートファジー / 放射線増感剤 / 膵臓癌 / クロロキン / 放射線照射 / 増感剤 / ドラッグリポジショニング |
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| Outline of Final Research Achievements |
As a treatment method for pancreatic cancer, which is an intractable malignant tumor, we focused on radiation resistance by autophagy and searched for the radiosensitizing effect of chloroquine drug repositioning. Pancreatic cancer cells were used to form spheroids, which were irradiated and administered with chloroquine in both normal medium and undernutrition, but there was no significant change in survival rate or size due to chloroquine, and no radiation sensitizing effect was observed.Moreover, the autophagy inhibitory effect was not clear. However, another study suggested an inhibitory effect on mitochondrial autophagy, mitophagy.
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| Academic Significance and Societal Importance of the Research Achievements |
膵癌の多くは切除不能であり、放射線治療抵抗性で予後不良であることから放射線増感効果に着目した抗腫瘍効果の増強を探索する試みは重要と考える。オートファジーに着目した放射線増感剤の探索、機序解明を進めることで、放射線生物学的にも重要な研究と考えている。
本研究ではクロロキンによる増感効果、オートファジー阻害効果を明らかにすることはできなかったが、マイトファジーの阻害作用が示唆された。
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