Control of intractable herpes simplex virus infections in immunocompromised children in Japan
| Project/Area Number |
18K07894
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 52050:Embryonic medicine and pediatrics-related
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
SAIJO MASAYUKI 国立感染症研究所, ウイルス第一部, 部長 (50300926)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 単純ヘルペスウイルス1型 / 水痘帯状疱疹ウイルス / 抗ウイルス薬 / アシクロビル / 耐性 / 薬剤感受性 / Bウイルス / 中枢神経感染症 / 単純ヘルペスウイルス１型 / Bウイルス感染症 / 脳炎 / 遺伝子検査法 / 単純ヘルペスウイルス / 薬剤耐性 / 免疫不全 / 薬剤感受性試験 / 免疫不全患者 |
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| Outline of Final Research Achievements |
The specimens collected from patients with intractable herpes simplex virus infections, varicella zoster virus infections, and cytomegalovirus infections were tested for sensitivity to antiviral agents. New mechanism of action for ACV-resistance that a mutation in the UL29 gene confers ACV-resistance was elucidated for the first time. HSV-1, which has a stop codon at the 44th position between the first and the second methionine, showed ACV-resistance, because the expression level of the viral thymidine kinase was reduced in a temperature dependent manner.
Two patients with neurological symptoms were retrospectively diagnosed as having B virus infections by detection of B virus-specific genome in the cerebrospinal fluid. These cases are the patients diagnosed with B virus infection for the first time in Asia as well as in Japan.
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| Academic Significance and Societal Importance of the Research Achievements |
免疫不全患者における薬剤耐性ヘルペスウイルス感染症の診断と治療の重要性はあまり認識されていない．国内で難治性ヘルペスウイルス感染症に関するウイルス学的な検査を実施できる施設は極めて限られている．これは造血幹細胞移植患者を含む免疫不全患者におけるヘルペスウイルス感染症への適切な診断と治療を難しくしている．本研究を通じて薬剤耐性ヘルペスウイルス感染症患者が少なからず存在することが再確認された．ヘルペスウイルス薬剤感受性試験を実施できる体制を構築する必要がある．
これまで欧米（特に米国）でのみ確認されていたサル由来Bウイルスによる脳炎患者が日本でも確認された．検査法および治療法開発が必要である．
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] New Mechanism of Acyclovir Resistance in Herpes Simplex Virus 1, Which Has a UAG Stop Codon between the First and Second AUG Initiation Codons2020
Author(s)
Nguyen PHA, Yamada S, Shibamura M, Inagaki T, Fujii H, Harada S, Fukushi S, Mizuguchi M, Saijo M
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Journal Title
Japanese Journal of Infectious Diseases
Volume: 73
Issue: 6
Pages: 447-451
DOI
NAID
ISSN
1344-6304, 1884-2836
Year and Date
2020-11-30
Related Report
Peer Reviewed / Open Access
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[Journal Article] Characterization of a Novel Alphaherpesvirus Isolated from the Fruit Bat Pteropus lylei in Vietnam2020
Author(s)
Inagaki T, Yamada S, Fujii H, Yoshikawa T, Shibamura M, Harada S, Fukushi S, Le MQ, Nguyen CT, Nguyen TTT, Nguyen TT, Nguyen TT, Quach VT, Thong VD, Mori K, Sasaki M, Setiyono A, Handharyani E, Takeyama H, Hasebe F, Saijo M
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Journal Title
Journal of Virology
Volume: 94
Issue: 18
DOI
Related Report
Peer Reviewed
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