Identification of the budding site of hepatitis B virus infectious particles (Dane particles)
Project/Area Number |
18K08004
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 53010:Gastroenterology-related
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Research Institution | Konan Women's University |
Principal Investigator |
Hotta Haku 甲南女子大学, 医療栄養学部, 教授 (40116249)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | B型肝炎ウイルス(HBV) / 感染性粒子(Dane粒子) / エンベロープリン脂質 / 出芽・放出部位 / 小胞体(ER) / 多胞体(MVB) / エクソソーム / 電子顕微鏡 / 出芽部位 / 細胞外放出 / 酵素親和性 / 多小胞体(MVB) / B型肝炎ウイルス / 感染性粒子(Dane粒子) / 小胞体(ER) / 多小胞体(MVB) |
Outline of Final Research Achievements |
We investigated the possible budding site(s) of the hepatitis B virus (HBV) virions as well as their release from the infected cells and the transmission to the uninfected target cells by means of their phospholipase A2 susceptibility, virion density, electron microscopic observations, knockdown experiments of the molecules involved in the functions of the endoplasmic reticulum (ER) and multivesicular body (MVB), and the exosome. We also performed comparative analyses between HBV and hepatitis C virus (HCV) as well as HBV and hepatitis D virus (HDV). Our results clearly demonstrated that the viral envelope properties differed between HBV and HCV, and that the uncoating process of the viral particles differed between HBV and HDV. Our results also suggested that the exosome pathway is involved in the release and transmission of HBV infectivity from the infected cells to the uninfected target cells. The precise determination of the HBV budding site(s) remains to be investigated.
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Academic Significance and Societal Importance of the Research Achievements |
B型肝炎ウイルス(HBV)とC型肝炎ウイルス(HCV)の感染性は、ある種のホスホリパーゼA2により非常に効率よく中和されるが、中和の機序は、HCVではエンベロープ破壊であるのに対して、HBVでは脱殻阻害であり、両ウイルスで明らかに異なることを実証した。また、HBVとD型肝炎ウイルス(HDV)は同じエンベロープを有しているが、細胞内侵入後の脱殻の過程が、両ウイルスで明らかに異なることを実証した。さらに、HBV感染細胞からの感染伝播に、HBV粒子やHBVゲノムを包含するエクソソームが関与することを実証した。これらの研究成果は、HBVのウイルス学的性質を明らかにするうえで重要なものと考えられる。
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Prevalence and genotype distribution of hepatitis B virus among migrant workers in Lombok Island, Indonesia2020
Author(s)
Yamani LN, Triani E, Amin M, Juniastuti, Utsumi T, Soetjipto, Nasronudin, Yano Y, Hotta H, Hayashi Y, Lusida MI.
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Journal Title
Asian Pacific Journal of Tropical Medicine
Volume: 13
Pages: 8-16
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] revalence and genotype distribution of hepatitis B virus among migrant workers in Lombok Island, Indonesia2020
Author(s)
2.Yamani LN, Triani E, Amin M, Juniastuti, Utsumi T, Soetjipto, Nasronudin, Yano Y, Hotta H, Hayashi Y, Lusida MI
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Journal Title
Asian Pacific Journal of Tropical Medicine
Volume: 13
Issue: 1
Pages: 8-16
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Peroxiredoxin 1, a novel HBx-interacting protein, interacts with exsome component 5 and negatively regulates hepatitis B virus (HBV) propagation through degradation of HBV RNA.2019
Author(s)
Lin Deng, Xiang Gan, Masahiko Ito, Ming Chen, Hussein H. Aly, Chieko Matsui, Takayuki Abe, Koichi Watashi,Takaji Wakita, Tetsuro Suzuki, Toru Okamoto, Yoshiharu Matsuura, Masashi Mizokami, Ikuo Shoji, and Hak Hotta.
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Journal Title
J. Virol
Volume: 93
Issue: 6
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 2.Further analysis of possible antiviral activity of CM-II-sPLA2 against HBV, HCV and HDV2019
Author(s)
Hotta H, Aoki-Utsubo C, Chen M, Nishimoto S, Deng L, Miyayama Y, Hijikata M, Shindo K, Noda T, Kohara M, Tsukuda S, Watashi K, Wakita T
Organizer
The 2019 International HBV Meeting
Related Report
Int'l Joint Research
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[Presentation] Comparative analysis of antiviral activity of sPLA2 against HBV and HCV2018
Author(s)
Hotta H, Chen M, Aoki-Utsubo C, Deng L, Miyayama Y, Hijikata M, Shindo K, Noda T, Kohara M, Watashi K, Wakita T
Organizer
2018 International HBV Meeting
Related Report
Int'l Joint Research
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[Presentation] Effect of antiviral activity of sPLA2 against HBV and HCV2018
Author(s)
Miyayama Y, Chen M, Aoki-Utsubo C, Deng L, Shindo K, Noda T, Kohara M, Watashi K, Wakita T, Hijikata M, Hotta H
Organizer
第66回日本ウイルス学会学術集会
Related Report
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