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Mucosa-associated microbiota and bacteriophage in the pathophysiology of irritable bowel syndrome (IBS)

Research Project

Project/Area Number 18K08020
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 53010:Gastroenterology-related
Research InstitutionKawasaki Medical School

Principal Investigator

Shiotani Akiko  川崎医科大学, 医学部, 教授 (80275354)

Co-Investigator(Kenkyū-buntansha) 内藤 裕二  京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00305575)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords粘膜細菌叢 / 過敏性腸症候群 / 酪酸産生菌 / 下痢型過敏性腸症候群 / 便秘型過敏性腸症候群 / TNF-α / IL-4 / Prevotella / Lachnospiraceae / サイトカイン / 腸内細菌叢 / 次世代シークエンサー / 食事アレルギー / 血清サイトカイン
Outline of Final Research Achievements

The aim of this study wasto investigate the MAM in IBS patients including the difference in subtypes of IBS, namely, diarrhea-predominant IBS (IBS-D) and constipation-predominant IBS (IBS-C). Endoscopic brush samples were taken from terminal ileum and sigmoid colon of patients with IBS (17 IBS-D patients and 7 IBS-C patients) and 10 healthy controls. The MAM of samples was profiled by 16S rRNA gene amplicon sequencing. Potential changes in the MAM at the functional level were evaluated using PICRUSt software and the KEGG database. The abundance of 4 genera in the sigmoid colon and 7 genera in the terminal ileum were significantly different among the 3 groups. In addition, the proportion of genes at the functional level differed between the IBS-D group and the IBS-C group. Dysbiosis pattern and the function of the microbiome seem to be different among subtypes of IBS, and MAM may play a crucial role in IBS symptom generation.

Academic Significance and Societal Importance of the Research Achievements

本研究により、酪酸産生菌が過敏性腸症候群(IBS)で有意に少く、便秘型IBS-Cと下痢型IBS-D間で粘膜細菌叢(MAM)の細菌構成比および機能解析に差を認めることを報告した。酪酸は、腸管上皮のアポトーシス抑制やバリア強化に関わり、過剰な免疫応答を抑制し、腸管免疫系の形成に重要な役割を果たすことが報告されている。しかしIBSサブタイプ別にMAMに注目し、検討した報告はほとんどなく、細菌代謝産物を治療に応用した報告はない。MAM代謝産物と病態あるいは重症度との関連性をさらに確認し、それらの効果を動物モデルで確認することにより、IBS新規治療法の開発につながる可能性がある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (19 results)

All 2020 2019 2018

All Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (10 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Mucosa-Associated Microbiota in Patients with Irritable Bowel Syndrome: A Comparison of Subtypes2020

    • Author(s)
      Matsumoto Hiroshi、Shiotani Akiko、Katsumata Ryo、Fukushima Shinya、Handa Yukiko、Osawa Motoyasu、Murao Takahisa、Handa Osamu、Umegaki Eiji、Inoue Ryo、Naito Yuji
    • Journal Title

      Digestion

      Volume: 102 Issue: 1 Pages: 49-56

    • DOI

      10.1159/000512167

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The Cutting Edge Research of Functional Gastrointestinal Disorders in Japan: Review on JGA Core Symposium 2018?20202020

    • Author(s)
      Sugiyama Toshiro、Shiotani Akiko
    • Journal Title

      Digestion

      Volume: 102 Issue: 1 Pages: 6-11

    • DOI

      10.1159/000510680

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Carcinogenesis and Gut Microbiota2019

    • Author(s)
      Shiotani A, Fukushima S, Matsumoto H.
    • Journal Title

      Gan To Kagaku Ryoho.

      Volume: 46 Pages: 199-204

    • Related Report
      2019 Research-status Report
  • [Journal Article] Analysis of the colonic mucosa associated microbiota (MAM) using brushing samples during colonic endoscopic procedures.2019

    • Author(s)
      Matsumoto H, Kuroki Y, Higashi S, Goda K, Fukushima S, Katsumoto R, Oosawa M, Murao T, Ishii M, Oka K, Takahashi M, Osaki T, Kamiya S, Shiotani A.
    • Journal Title

      J Clin Biochem Nutr

      Volume: 65 Pages: 132-137

    • NAID

      130007700222

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Current and Future Treatment of Helicobacter pylori Infections2019

    • Author(s)
      Matsumoto H, Shiotani A, DY Graham
    • Journal Title

      Adv Exp Med Biol

      Volume: 1149 Pages: 211-225

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Carcinogenesis and Gut Microbiota2019

    • Author(s)
      Akiko Shiotani
    • Journal Title

      Gan To Kagaku Ryoho

      Volume: 46 Pages: 199-204

    • Related Report
      2018 Research-status Report
  • [Journal Article] The TPH1 rs211105 gene polymorphism affects abdominal symptoms and quality of life of diarrhea-predominant irritable bowel syndrome2018

    • Author(s)
      Katsumata R, Shiotani A, Murao T, Ishii M, Fujita M, Matsumoto H, Haruma K.
    • Journal Title

      Journal of Clinical Biochemistry and Nutrition

      Volume: 62 Issue: 3 Pages: 270-276

    • DOI

      10.3164/jcbn.17-76

    • NAID

      130006730773

    • ISSN
      0912-0009, 1880-5086
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cytokine Profile and Immunoglobulin E-mediated Serological Food Hypersensitivity in Patients With Irritable Bowel Syndrome With Diarrhea.2018

    • Author(s)
      Katsumata R, Ishii M, Lee S, Handa Y, Murao T, Fujita M, Matsumoto H, Otsuki T, Shiotani A.
    • Journal Title

      J Neurogastroenterol Motil

      Volume: 30 Pages: 415-421

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Mucosa-Associated Intestinal Micobiota And Serum Cytokines In Patients With Diarrhea Predominant Irritabe Bowel Syndrome And Ige Mediated Hypersensitivity2020

    • Author(s)
      Shiotani Akiko, Katsumata Ryo, Fukushima SHinya, Handa Yukiko, Goda Kyosuke, Oosawa Motoyasu, Murao Takahisa, Handa Osamu, Matsumoto Hiroshi, Umegaki Eiji, Inoue Ryo, Naito Yuji
    • Organizer
      Digestive Disease Week 2020(AGA)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 消化管機能性疾患の新展開 消化管機能性疾患の現状と将来 過敏性腸疾患(IBS)における腸粘膜細菌叢(Mucosa-associated Microbiata:MAN)の解析2020

    • Author(s)
      松本啓志, 塩谷昭子, 勝又諒, 福嶋真弥, 大澤元保, 村尾高久, 半田修, 梅垣英次, 井上亮, 内藤裕二
    • Organizer
      第16回日本消化管学会総会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 過敏性腸症候群(IBS)における腸粘膜細菌叢(Mucosa-associated Microbiota:MAM)の解析2020

    • Author(s)
      松本啓志、塩谷昭子、勝又諒
    • Organizer
      第16回日本消化管学会総会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Mucosa-associated intestinal microbiota and serum cytokines in patients with diarrhea predominant irritable bowel syndrome and IgE mediated hypersensitivity2020

    • Author(s)
      Akiko Shiotani, Ryo Katsumata, Shinya Fukushima, Yukiko Handa, Kyosuke Goda, Motoyasu Oosawa, Takahisa Muroa, Osamu Handa, Hiroshi Matsumoto, Eiji Umegaki, Ryo Inoue, Yuji Naito
    • Organizer
      Digestive Disease Week (DDW) 2020 米国シカゴ
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 消化器領域における腸内細菌研究と臨床応用ブラシ法による腸粘膜関連マイクロバイオータ(mucosal associated microbiota:MAM)の解析2019

    • Author(s)
      松本啓志, 塩谷昭子
    • Organizer
      第105回日本消化器病学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] 下痢型過敏性腸症候群粘膜における粘膜関連腸内細菌叢と食物アレルギー及び血清サイトカイン値の関係性2019

    • Author(s)
      半田有紀子、 勝又 諒、塩谷昭子
    • Organizer
      JDDW 2019
    • Related Report
      2019 Research-status Report
  • [Presentation] 勝又諒, 福嶋真弥, 半田有紀子, 合田杏佑, 石井学, 村尾高久, 李 順姫, 松本啓志, 大槻剛巳, 塩谷昭子2019

    • Author(s)
      "「消化管機能性疾患の新展開」過敏性腸症候群の分子病態と新展開 下痢型過敏性腸症候群と炎症性腸疾患における食物アレルギーの関与とサイトカインプロファイル"
    • Organizer
      第15回日本消化管学会総会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Cytokine profile and ige-mediated serological food hypersensitivity in patients with irritable bowel syndrome with diarrhea2018

    • Author(s)
      Katsumata Ryo, Ishii Manabu, Murao Takahisa , Fujita Minoru, Matsumoto Hiroshi, Shiotani Akiko
    • Organizer
      Digestive Disease Week 2018(AGA)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Serum Cytokine profile of Patients with Functional Dyspepsia2018

    • Author(s)
      Katsumata Ryo, Handa Yukiko, Gouda Kyousuke, Fukushima Shinya, Lee Suni, Ishii Manabu, Murao Takahisa , Matsumoto Hiroshi, Otsuki Takemi, Shiotani Akiko
    • Organizer
      Asian Pacific Digestive Week 2018 (APDW 2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] "Current understanding of peptic ulcer disease GI bleeding in the era of direct oral anticoagulants"2018

    • Author(s)
      Shiotani Akiko
    • Organizer
      Asian Pacific Digestive Week(APDW 2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] Gastric cancer2019

    • Author(s)
      AKIKO SHIOTANI
    • Total Pages
      201
    • Publisher
      Springer
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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