Enteric Nervous System in Congenital Intestinal Disease
| Project/Area Number |
18K08537
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 55010:General surgery and pediatric surgery-related
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
Sakai Sachiko 滋賀医科大学, 医学部, 助教 (10710478)
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| Co-Investigator(Kenkyū-buntansha) |
久保田 良浩 滋賀医科大学, 医学部, 非常勤講師 (30305601)
加藤 久尚 滋賀医科大学, 医学部, 医員 (40756756)
谷 眞至 滋賀医科大学, 医学部, 教授 (60236677)
清水 智治 滋賀医科大学, 医学部, 教授 (70402708)
田尻 達郎 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80304806)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Hirschsprung病診断 / calretinin免疫染色 / choline transporter免疫染色 / Calretinin免疫染色 / Choline trasporter免疫染色 / Choline acetyltranferase / PGP9.5 / 一酸化窒素合成酵素 / Cholintransporter免疫染色 / Cholin Acetyltransferase / Choline transporter免疫染色 / Acetylcholinesterase染色 / 腸管神経系システム / 先天性腸疾患 / Hirschsuprung病 |
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| Outline of Final Research Achievements |
Calretinin(CR) immunostaining and Choline transporter(ChT) immunostaining were performed on intestinal specimens from neonates to 3 months of age in cases of Hirschsprung's disease(HD) and non-HD, respectively. CR immunostaining was positive in all cases of non-HD and negative in all cases of HD, even in the early neonatal period and in preterm infants, with a sensitivity and specificity of 100%; ChT immunostaining was negative in all cases of non-HD intestinal tracts, but the positive findings were difficult to recognize, with a sensitivity of 50% and specificity of 100%. Thus, CR immunostaining is useful in the diagnosis of HD, even in the early neonatal period, but the usefulness of ChT immunostaining is negative.
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| Academic Significance and Societal Importance of the Research Achievements |
H病診断のための直腸粘膜生検に対するAcetylcholinesterase染色（AChE染色）は、正診率が高いが、新生児早期では、粘膜固有層にAChE陽性である外来神経線維の代償性の増生がまだみられず、偽陰性になりうることが問題であった。
今回の研究結果から、新生児期早期であっても、非H病腸管ではCR染色が陽性となり、H病ではCR染色陰性となることが確認できた。従来のAChE染色単独では不十分であった新生児期早期においても、AChE染色の補助診断としてCR染色を併用することで、より正確にH病診断を行えることが期待できる。
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Report
(5 results)
Research Products
(1 results)
