• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Attempt to create a peristaltic functional artificial intestine using human-derived stem cells and decellularization technology

Research Project

Project/Area Number 18K08600
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 55010:General surgery and pediatric surgery-related
Research InstitutionKeio University

Principal Investigator

Takumi Fujimura  慶應義塾大学, 医学部(信濃町), 助教 (80573443)

Co-Investigator(Kenkyū-buntansha) 黄地 健仁  慶應義塾大学, 医学部(信濃町), 助教 (30803564)
黒田 達夫  慶應義塾大学, 医学部(信濃町), 教授 (60170130)
芝田 晋介  慶應義塾大学, 医学部(信濃町), 訪問教授 (70407089)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords腸管運動不全 / 人工腸管 / 歯髄細胞 / 神経堤細胞 / 発光イメージング / 腸管不全 / 脱細胞技術 / 幹細胞 / 再生医療 / 人工臓器 / 小腸移植
Outline of Final Research Achievements

As a new treatment for short bowel syndrome and intestinal dismotility, we aimed to construct a functional artificial small intestinal graft which made by decellularization technology and human dental pulp cells. A rat small intestine scaffold could be prepared by decellularization technology, and the properties of the human dental pulp cells to be transplanted could be confirmed. However, in recellarization by transvascular circulation culture (2 weeks), most of the transplanted cells stayed in the mesenterica in the circulation culture route. Immunohistochemistry could not prove the transplanted cells in the intestinal scaffold. Only a few cells could be confirmed by scanning electron microscope. We tried to extend the circulation culture for 4 weeks, but it did not lead to an improvement in the engraftment rate.

Academic Significance and Societal Importance of the Research Achievements

短腸症候群や腸管不全症例に対する根本的治療は小腸・多臓器移植であるが、厳しい免疫抑制を要する治療とドナー不足の問題を抱えている。蠕動しない腸管はうっ滞性腸炎の温床になる危険性を高めるだけであり、これらの問題点を解決するためには自家細胞を用いた免疫抑制を必要としない、蠕動機能を備えた人工臓器の作成を脱細胞技術を用いて作製する必要があると考えた。脱細胞技術を用いる利点は移植時に吻合する血管や腸管内の微細な血管構造を備えており、移植医療に最適と考えたためであるが類洞のようなもともと細胞通過を許容するような構造を備えた肝臓とは異なり、腸管では血管経由以外の再細胞化の方法を検討する必要性が示唆された。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (7 results)

All 2021 2020 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Maintenance treatment with infliximab for ulcerative ileitis after intestinal transplantation - a case report and literature review2021

    • Author(s)
      Takumi Fujimura
    • Journal Title

      World Journal of Clinical Cases

      Volume: -

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 脱細胞技術と幹細胞が拓く腸管再生医療2021

    • Author(s)
      藤村 匠
    • Organizer
      第58回日本小児外科学会総会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 幹細胞と脱細胞技術を用いた人工小腸作成の試み2020

    • Author(s)
      藤村 匠
    • Organizer
      第50回日本小児消化管機能研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 幹細胞と脱細胞技術を用いた人工小腸作成の試み2020

    • Author(s)
      藤村 匠
    • Organizer
      第50回消化管機能研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] 幹細胞と脱細胞技術を用いた人工小腸作成の試み2018

    • Author(s)
      藤村 匠
    • Organizer
      第31回小腸移植研究会
    • Related Report
      2018 Research-status Report
  • [Book] Hirschsprung’s Disease and the Allied Disorders: Status Quo and Future Prospects of Treatment2019

    • Author(s)
      Takumi Fujimura
    • Total Pages
      292
    • Publisher
      Springer
    • ISBN
      9811336059
    • Related Report
      2018 Research-status Report
  • [Book] 小児外科2018

    • Author(s)
      藤村 匠
    • Total Pages
      107
    • Publisher
      東京医学社
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi