Quick detection of causative bacteria in cases of acute cholangitis and cholecystitis using a multichannel gene autoanalyzer

• Project/Area Number: 18K08633
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Toho University
• Principal Investigator: ASAI Koji 東邦大学, 医学部, 准教授 (20366454)
• Co-Investigator(Kenkyū-buntansha): 鯨岡 学 東邦大学, 医学部, 助教 (00807092) ; 片桐 美和 東邦大学, 医学部, 助教 (90385667)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 急性胆道炎 / 全自動遺伝子検査システム / 胆汁細菌 / メタゲノム解析 / 自動多項目同時遺伝子検出 / 抗菌薬感受性 / 多剤耐性菌 / 自動多項目同時遺伝子システム / 新規起因菌同定法

Outline of Final Research Achievements

Purposes: In this study, we evaluate the use of a multichannel gene autoanalyzer (Verigene system) for the quick detection of causative bacteria in cases of acute cholangitis and cholecystitis. Methods: This study included 108 patients diagnosed with acute cholangitis or cholecystitis. Bacterial culture test and Verigene assay were used to evaluate the bile samples.
Results: Among patients with positive bile cultures, bacteria were detected in 35.7% of cases via the Verigene system. The detection rates of the Verigene system significantly increased when the number of bacterial colonies was 10^6 colony-forming unit (CFU)/mL (58.1%). Cases with a maximum colony quantity of　10^6 CFU/mL exhibited higher inflammation, suggesting the presence of bacterial infection. Conclusions: The Verigene system might be a new method for the quick detection of causative bacteria in patients with infectious acute cholangitis and cholecystitis.

Academic Significance and Societal Importance of the Research Achievements

これまで明らかとなっていなかった全自動遺伝子検査システム（Verigene）を使用し急性胆道炎症例の胆汁評価を行った．高い検出率を期待したが，細菌量が少ない場合や複数菌が検出された場合は検出率が低下した．
次世代の迅速細菌同定法の評価を行うことができ，今回の結果は今後の臨床応用に寄与できる可能性が示唆された．

Research Products

• [Journal Article] Quick detection of causative bacteria in cases of acute cholangitis and cholecystitis using a multichannel gene autoanalyzer (2021)
  • Author(s): Ryutaro Watanabe, Koji Asai, Makoto Kuroda, Manabu Kujiraoka, Tsuyoshi Sekizuka, Miwa Katagiri, Nanako Kakizaki, Hodaka Moriyama, Manabu Watanabe, Yoshihisa Saida
  • Journal Title: Surgery Today Volume: In Press
  • Peer Reviewed
• [Presentation] Rapid detection of causative bacteria in case of acute cholangitis and cholecystitis using a multichannel gene autoanalyzer (2021)
  • Author(s): Watanabe R, Asai K, Kujiraoka M, Moriyama H, Watanabe M
  • Organizer: 第32回日本肝胆膵外科学会学術集会
• [Presentation] Early laparoscopic cholecystectomy using the bailout procedure for acute cholecystitis with severe local inflammation (2021)
  • Author(s): Asai K, Watanabe M, Kujiraoka M, Moriyama H, Watanabe R
  • Organizer: 第32回日本肝胆膵外科学会学術集会
• [Presentation] 急性胆嚢炎に対する早期腹腔鏡下胆嚢摘出術-積極的にFundus first techniqueを行っている当科の治療成績 (2021)
  • Author(s): 浅井浩司，渡邉 学，森山穂高，鯨岡 学，渡邉隆太郎，柿崎奈々子，寺岡晋太郎，榎本俊行，二渡信江，斉田芳久
  • Organizer: 第33回日本内視鏡外科学会総会
• [Presentation] 急性胆嚢炎に対する早期腹腔鏡下胆嚢摘出術を基軸とした治療戦略と自動多項目同時遺伝子検出システムを用いた早期起因菌同定法 (2020)
  • Author(s): 浅井浩司，黒田 誠，渡邉隆太郎，鯨岡 学，片桐美和，柿崎奈々子，森山穂高，榎本俊行，桐林孝治，二渡信江，長田拓哉，渡邉 学，斉田芳久
  • Organizer: 第33回日本外科感染症学会総会学術集会
• [Presentation] 急性胆道炎における自動多項目同時遺伝子検出システムによる早期起因菌同定法 (2020)
  • Author(s): 浅井浩司，黒田 誠，渡邉隆太郎，鯨岡 学，森山穂高，寺岡晋太郎，柿崎 奈々子，片桐美和，榎本俊行，桐林孝治，二渡信江，渡邉 学，斉田芳久
  • Organizer: 第120回日本外科学会定期学術集会
• [Presentation] Operative results for acute cholecystitis according to the operative timing using propensity score matching (2019)
  • Author(s): Asai K, Watanabe M, Kujiraoka M, Moriyama H, Kakizaki N, Saida Y
  • Organizer: 31th Meeting of Japanese Society of Hepato-Biliary-Pancreatic Surgery
• [Presentation] Comprehensive analysis of 103 cases of acute cholecystitis and cholangitis using metagenomic approach (2019)
  • Author(s): Kujiraoka M, Kuroda M, Asai K, Watanabe M, Moriyama H, Enomoto T, Saida Y
  • Organizer: The International College of Surgeons, The 65th Annual Congress of the Japanese Section
  • Int'l Joint Research
• [Presentation] A study of 103 cases with acute cholecystitis and cholangitis using multichannel gene autoanalyzer (2019)
  • Author(s): Watanabe R，Kuroda M, Kujiraoka M, Asai K, Watanabe M, Katagiri M, Moriyama H, Kakizaki N, Saida Y
  • Organizer: The International College of Surgeons, The 65th Annual Congress of the Japanese Section
  • Int'l Joint Research
• [Presentation] Instruction of laparoscopic cholecystectomy in acute cholecystitis using single-incisional laparoscopic surgery device (2019)
  • Author(s): Asai K, Watanabe M, Kujiraoka M, Moriyama H, Watanabe R, Kakizaki N, Teraoka S, Katagiri M, Enomoto T, Saida Y
  • Organizer: The 7th Biennial Congress of the Asian-Pacific Hepato-Pancreato-Biliary Association (A-PHPBA 2019)
  • Int'l Joint Research
• [Presentation] A study of 105 cases with acute cholecystitis and cholangitis using multichannel gene autoanalyzer (2019)
  • Author(s): Watanabe R, Kuroda M, Kujiraoka M, Asai K, Watanabe M, Katagiri M, Moriyama H, Kakizaki N, Saida Y
  • Organizer: The 7th Biennial Congress of the Asian-Pacific Hepato-Pancreato-Biliary Association (A-PHPBA 2019)
  • Int'l Joint Research
• [Presentation] Comprehensive diagnosis of 103 cases of acute cholecystitis and cholangitis using a metagenomic analysis (2019)
  • Author(s): Kujiraoka M, Kuroda M, Asai K, Watanabe M, Moriyama H, Watanabe R, Saida Y
  • Organizer: The 7th Biennial Congress of the Asian-Pacific Hepato-Pancreato-Biliary Association (A-PHPBA 2019)
  • Int'l Joint Research
• [Presentation] 特別企画1「新たに改訂されたTG18：ここがポイント」TG18 急性胆嚢炎の診療フローチャート (2019)
  • Author(s): 浅井浩司，高田忠敬，岡本好司
  • Organizer: 第55回日本胆道学会学術集会
• [Presentation] 単孔式内視鏡外科手術デバイスを用いた急性胆嚢炎に対する早期腹腔鏡下胆嚢摘出術 (2019)
  • Author(s): 浅井浩司，渡邉 学，鯨岡 学，森山穂高，渡辺隆太郎，柿崎奈々子，片桐美和，榎本俊行，桐林孝治，二渡信江，斉田芳久
  • Organizer: 第81回日本臨床外科学会総会
• [Presentation] 急性胆嚢炎に対して早期腹腔鏡下胆嚢摘出術を基本的治療方針としている当科の治療成績 (2019)
  • Author(s): 浅井浩司，渡邉 学，鯨岡 学，森山穂高，渡辺隆太郎，寺岡晋太郎，柿崎奈々子，片桐美和，榎本俊行，桐林孝治，斉田芳久
  • Organizer: 第32回日本外科感染症学会総会学術集会
• [Presentation] 急性胆嚢炎に対する早期腹腔鏡下胆嚢摘出術の治療成績と最近の工夫 (2019)
  • Author(s): 浅井浩司，渡邉 学，鯨岡 学，森山穂高，渡辺隆太郎，柿崎奈々子，片桐美和，榎本俊行，桐林孝治，二渡信江，斉田芳久
  • Organizer: 第32回日本内視鏡外科学会総会
• [Presentation] 急性胆嚢炎の腹腔鏡下胆嚢摘出術における開腹移行の危険因子に関する検討 (2019)
  • Author(s): 浅井浩司，渡邉 学，鯨岡 学，森山穂高，渡辺隆太郎，柿崎奈々子，片桐美和，榎本俊行，桐林孝治，二渡信江，斉田芳久
  • Organizer: 第32回日本内視鏡外科学会総会
• [Presentation] 急性胆嚢炎に対する安全な早期腹腔鏡下胆嚢摘出術を目指したわれわれの工夫 (2019)
  • Author(s): 浅井浩司，渡邉 学，齋藤智明，鯨岡 学，森山穂高，片桐美和，榎本俊行，桐林孝治，二渡信江，斉田芳久，草地信也
  • Organizer: 第55回日本腹部救急医学会総会
• [Presentation] Timing of laparoscopic cholecystectomy for acute cholecystitis using propensity score matching (2018)
  • Author(s): Asai K, Watanabe M, Saito T, Kujiraoka M, Moriyama H, Katagiri M, Enomoto T, Saida Y, Kusachi S
  • Organizer: International Association of Surgeons, Gastroenterologists and Oncologists Continuing Medical Education: Advanced Post-Graduate Course in Tokyo 2018
  • Int'l Joint Research
• [Presentation] Comprehensive diagnosis and severity evaluation for acute cholecystitis using metagenomic approach (2018)
  • Author(s): Kujiraoka M, Kuroda M, Asai K, Watanabe M, Matsukiyo H, Saito T, Moriyama H, Saida Y, Kusachi S
  • Organizer: International Association of Surgeons, Gastroenterologists and Oncologists Continuing Medical Education: Advanced Post-Graduate Course in Tokyo 2018
  • Int'l Joint Research
• [Presentation] Comprehensive diagnostic method for acute cholecystitis using metagenomic approach (2018)
  • Author(s): Kujiraoka M, Kuroda M, Asai K, Watanabe M, Matsukiyo H, Saito T, Moriyama H, Enomoto T, Okamoto Y, Saida Y, Kusachi S
  • Organizer: The International College of Surgeons, The 64st Annual Congress of the Japanese Section
  • Int'l Joint Research
• [Presentation] Instruction of laparoscopic cholecystectomy in acute cholecystitis using single-incisional laparoscopic surgery device (2018)
  • Author(s): Asai K, Watanabe M, Saito T, Kujiraoka M, Moriyama H, Enomoto T, Saida Y, Kusachi S
  • Organizer: The 13th World Congress of the International Hepato-Pancreato-Biliary Association
  • Int'l Joint Research
• [Presentation] Comprehensive analysis for acute cholecystitis using metagenomic approach (2018)
  • Author(s): Kujiraoka M, Kuroda M, Asai K, Watanabe M, Matsukiyo H, Saito T, Moriyama H, Saida Y, Kusachi S
  • Organizer: The 13th World Congress of the International Hepato-Pancreato-Biliary Association
  • Int'l Joint Research
• [Presentation] 急性胆嚢炎に対する早期腹腔鏡下胆嚢摘出術の現状と安全性の評価 (2018)
  • Author(s): 浅井浩司，渡邉 学，齋藤智明，鯨岡 学，森山穂高，片桐美和，榎本俊行，桐林孝治，二渡信江，斉田芳久，草地信也
  • Organizer: 第80回日本臨床外科学会総会
• [Presentation] 手術困難とは？そして危険回避手術とは？ (2018)
  • Author(s): 浅井浩司，岩下幸雄，本田五郎，梅澤昭子，若林 剛，高田忠敬
  • Organizer: 第31回日本外科感染症学会総会学術集会
• [Presentation] 急性胆嚢炎に対して早期腹腔鏡下胆嚢摘出術を基本的治療方針としている当科の治療成績 (2018)
  • Author(s): 浅井浩司，渡邉 学，齋藤智明，鯨岡 学，森山穂高，片桐美和，榎本俊行，桐林孝治，二渡信江，斉田芳久，草地信也
  • Organizer: 第31回日本内視鏡外科学会総会
• [Presentation] 急性胆嚢炎に対する腹腔鏡下手術における単孔式腹腔鏡下手術用デバイスを応用した手術指導の工夫 (2018)
  • Author(s): 浅井浩司，渡邉 学，松清 大，齋藤智明，鯨岡 学，森山穂高，片桐美和，榎本俊行，斉田芳久，草地信也
  • Organizer: 第73回日本消化器外科学会総会
• [Presentation] 術者別に見た急性胆嚢炎に対する腹腔鏡下胆嚢摘出術の治療成績の検討 (2018)
  • Author(s): 浅井浩司，渡邉 学，松清 大，齋藤智明，鯨岡 学，森山穂高，片桐美和，榎本俊行，斉田芳久，草地信也
  • Organizer: 第16回日本消化器外科学会大会