The basic research about cancer heterogeneity using 3D and co-culture for precision medicine
Project/Area Number |
18K08687
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 55020:Digestive surgery-related
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Research Institution | Kagoshima University |
Principal Investigator |
KITA YOSHIAKI 鹿児島大学, 医歯学域鹿児島大学病院, 助教 (30570692)
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Co-Investigator(Kenkyū-buntansha) |
盛 真一郎 鹿児島大学, 医歯学域鹿児島大学病院, 助教 (00620519)
田辺 寛 鹿児島大学, 鹿児島大学病院, 医員 (40814306)
関 直彦 千葉大学, 大学院医学研究院, 准教授 (50345013)
夏越 祥次 鹿児島大学, 医歯学域医学系, 教授 (70237577)
伊地知 徹也 鹿児島大学, 鹿児島大学病院, 医員 (70791531)
大迫 祐作 鹿児島大学, 鹿児島大学病院, 医員 (80791532)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | heterogeneity / 3D培養 / オルガノイド / 癌幹細胞 / 治療抵抗性 / Notch / 3D培養 |
Outline of Final Research Achievements |
We have established 3D human cancer organoid of esophageal squamous cell carcinoma o from biopsy sample for the first time in the world. Moreover, we have published above in detail. The result of NGS analysis from co-culture of organoid highlight some noble non-coding RNA(mir-145-3p ,miR-130b-5p, miR-148a-5p, miR-204-5p,miR-145-5p, and miR-143-3p), and then, we disclosed the function of them.
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Academic Significance and Societal Importance of the Research Achievements |
本研究において、一般的なオルガノイド培養から一歩踏み込んだ共培養を用いたオルガノイド培養を行うことで,腫瘍先進部とこのオルガノイドの性質が一部近似することが判明し,今後,腫瘍オルガノイドから最も悪性度に反映する腫瘍先進部を予測・解析することができれば,PDX(Patient Derived Xenograft)よりコストと時間を大幅に削減でき,癌組織の不均一性解明の一助となり,個別化治療における治療選択・予後予測に繋がると考えられる.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Clinical prospects for laparoscopic stoma closure of a temporary loop ileostomy: Initial experience and report.2020
Author(s)
Kita Y, Mori S, Tanabe K, Baba K, Tanoue K, Idichi T, Wada M, Arigami T, Sasaki K, Maemura K, Natsugoe S.
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Journal Title
Asian J Endosc Surg.
Volume: 13
Issue: 4
Pages: 618-621
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A long-term survivor of recurrent esophagogastric junction adenocarcinoma treated with multidisciplinary therapy: a case report.2020
Author(s)
Tanaka T, Arigami T, Uenosono Y, Yanagita S, Matsushita D, Okubo K, Kijima T, Uchikado Y, Kita Y, Mori S, Sasaki K, Omoto I, Kurahara H, Maemura K, Ishigami S, Natsugoe S.
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Journal Title
Surg Case Rep
Volume: 6
Issue: 1
Pages: 13-13
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Indication and Prognostic Significance of Conversion Surgery in Patients with Liver Metastasis from Gastric Cancer.2020
Author(s)
Arigami T, Matsushita D, Okubo K, Kawasaki Y, Iino S, Sasaki K, Noda M, Kita Y, Mori S, Kurahara H, Maemura K, Yanagita S, Uenosono Y, Ishigami S, Natsugoe S
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Journal Title
Oncology
Volume: 1
Issue: 5
Pages: 1-7
DOI
Related Report
Peer Reviewed
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[Journal Article] Regulation of aberrantly expressed SERPINH1 by antitumor miR-148a-5p inhibits cancer cell aggressiveness in gastric cancer.2020
Author(s)
Kawagoe K, Wada M, Idichi T, Okada R, Yamada Y, Moriya S, Okubo K, Matsushita D, Arigami T, Kurahara H, Maemura K, Natsugoe S, Seki N.
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Journal Title
J Hum Genet
Volume: -
Issue: 8
Pages: 647-656
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation.2019
Author(s)
Khalid M, Idichi T, Seki N, Wada M, Yamada Y, Fukuhisa H, Toda H, Kita Y, Kawasaki Y, Tanoue K, Kurahara H, Mataki Y, Maemura K, Natsugoe S.
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Journal Title
Cancers (Basel)
Volume: 11
Issue: 3
Pages: 327-327
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Gene regulation by antitumor miR-130b-5p in pancreatic ductal adenocarcinoma: the clinical significance of oncogenic EPS8.2019
Author(s)
Fukuhisa H, Seki N, Idichi T, Kurahara H, Yamada Y, Toda H, Kita Y, Kawasaki Y, Tanoue K, Mataki Y, Maemura K, Natsugoe S.
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Journal Title
J Hum Genet.
Volume: -
Issue: 6
Pages: 521-534
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] adial incision and cutting method using a transanal approach for treatment of anastomotic strictures following rectal cancer surgery: a case report.2019
Author(s)
Nepal P, Mori S, Kita Y, Tanabe K, Baba K, Uchikado Y, Kurahara H, Arigami T, Sakoda M, Maemura K, Natsugoe S.
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Journal Title
World J Surg Oncol.
Volume: 17
Issue: 1
Pages: 48-48
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Combined endoscopic submucosal dissection and transanal minimally invasive surgery for the management of lower rectal adenoma extending above the dentate line: A case report.2019
Author(s)
Nepal P, Mori S, Kita Y, Tanabe K, Baba K, Sasaki F, Nasu Y, Ido A, Uchikado Y, Kurahara H, Arigami T, Sakoda M, Maemura K, Natsugoe S
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Journal Title
Medicine (Baltimore)
Volume: 98
Issue: 19
Pages: e15289-e15289
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Combined neutrophil-lymphocyte ratio and platelet-lymphocyte ratio predicts chemotherapy response and prognosis in patients with advanced gastric cancer.2019
Author(s)
Hirahara T, Arigami T, Yanagita S, Matsushita D, Uchikado Y, Kita Y, Mori S, Sasaki K, Omoto I, Kurahara H, Maemura K, Okubo K, Uenosono Y, Ishigami S, Natsugoe S.
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Journal Title
BMC Cancer
Volume: 19
Issue: 1
Pages: 672-672
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Molecular pathogenesis of esophageal squamous cell carcinoma: Identification of the antitumor effects of miR 145 3p on gene regulation.2019
Author(s)
Shimonosono M, Idichi T, Seki N, Yamada Y, Arai T, Arigami T, Sasaki K, Omoto I, Uchikado Y, Kita Y, Kurahara H, Maemura K, Natsugoe S.
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Journal Title
Int J Oncol.
Volume: 54
Pages: 673-688
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Three-stage laparoscopic surgery in a morbidly obese patient with Hinchey III diverticulitis: a case report.2019
Author(s)
Tanaka T, Kita Y, Mori S, Baba K, Tanabe K, Wada M, Tsuruda Y, Tanoue K, Yanagita S, Maemura K, Natsugoe S.
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Journal Title
Surg Case Rep.
Volume: 5
Issue: 1
Pages: 24-24
DOI
Related Report
Peer Reviewed / Open Access
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