| Project/Area Number |
18K08797
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 55040:Respiratory surgery-related
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| Research Institution | International University of Health and Welfare |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高後 裕 国際医療福祉大学, 大学病院, 教授 (10133183)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | lung cancer / screening / micro RNA / exosome / smoking / lung cancer screening / microRNA / 肺がん / 早期発見 |
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| Outline of Final Research Achievements |
[Purpose] We isolated urinary exosomes as a screening source for lung cancer (LC) and measured both expression of miroRNAs (miRs) and matrix metalloproteinase-1 (MMP-1). [Methods] Thirty-five LC patients and 40 healthy controls provided the first urines in the morning. The expression of miR21, miR-486-5p and the expression of MMP-1/CD 63 in urinary exosomes were measured by qRT-PCR and Western blotting, respectively. [Results] The mean expression levels of miR-486-5p in male LC patients showed significantly higher those of male healthy controls. Whereas that in female LC patients showed lower those of female healthy controls. The expression ratio of MMP-1/CD63 in 35 LC patients was significantly higher compared with those in 40 healthy controls.
[Conclusion] Combined expression of miR-486-5p and MMP-1/CD63 in urinary exosomes suggested the great advantage for non-smoker females to detect early stage adenocarcinoma.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果の最たる点は、より簡便に採取できる尿のexosome解析から肺がん早期発見に寄与する知見を得た点である。尿中exosomeに含まれるmicroRNAsとしてmiR-21、miR-486-5pを、oncogeneとしてmatrix metalloproteinase (MMP)-1を検討し、うちmiR-486-5pが女性肺癌で、MMP-1は非喫煙者肺がんで、早期発見に資する結果が得られた。昨今、増加傾向を示す非喫煙者、および女性肺癌の早期発見に役立つ可能性を示したと言える。
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