Relationship between intrauterine infection and endometriosis and its association with infertility
| Project/Area Number |
18K09268
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56040:Obstetrics and gynecology-related
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
カーン カレク 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (60336162)
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| Project Period (FY) |
2018-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | endometriosis / uterine infection / LVFX / GnRHa / LVFX/GnRHa / inflammation / metagenone / エンドメトリオーシス / メタゲノム / 子宮内膜症 / 腟内細菌叢 |
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| Outline of Annual Research Achievements |
This is a prospective non-randomized observational study to evaluate microbial profiles in endometrial samples that were collected from women with and without endometriosis. Endometrial and endometriotic samples were collected from 53 women with endometriosis and 47 control women and were treated with levofloxacin (LVFX, 500mg, once per os) or GnRHa (1.88mg/IM for 3 months) before surgery.
16S rDNA metagenome assay indicated that treatment with either of LVFX or GnRHa+LVFX significantly decreased some components of major bacterial genera comparing to untreated group.
Immunohistochemical analysis with antibody against CD138 indicated that the occurrence rate of chronic endometritis was significantly decreased after GnRHa+LVFX treatment comparing to GnRHa treatment group (P=0.041).
These findings suggest the effectiveness of a broad spectrum antibiotic, as a non-hormonal agent, in improving uterine infection in human endometriosis.
As an extended study, we evaluated association between ano-genital distance (AGD) (distance between anal rim and posterior fourchette of the vagina) and vaginal pH (an indicator of vaginal eubiosis and dysbiosis) in women with (n=67) and without (n=40) endometriosis. We found that there was no significant difference in the median AGD among control women (21.5mm) and women with either early (22.0mm) or advanced endometriosis (20.0mm).The univariate analysis showed that alkaline shifting of vaginal pH (more than 4.5) was associated with the presence of endometriosis (OR [95% CI] = 0.40 [0.17, 0.93], P=0.034) and was independent of the length of AGD.
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Report
(6 results)
Research Products
(53 results)
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[Journal Article] Is neonatal uterine bleeding responsible for early-onset endometriosis?2023
Author(s)
Ogawa Kanae、Khan Khaleque N、Kuroboshi Haruo、Koshiba Akemi、Shimura Koki、Tajiri Tatsuro、Fumino Shigehisa、Fujita Hiroyuki、Okubo Tomoharu、Fujiwara Yoichiro、Horiguchi Go、Teramukai Satoshi、Fujishita Akira、Itoh Kyoko、Guo Sun-Wei、Kitawaki Jo、Mori Taisuke
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Journal Title
Reproductive Biology and Endocrinology
Volume: 21
Issue: 1
Pages: 56-65
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Levofloxacin or GnRHa treatment decreases intrauterine microbial colonization in human endometriosis2021
Author(s)
Khan KN, Fujishita A, Muto H, Masumoto H, Ogawa K, Koshiba A, Mori T, Itoh K, Teramukai S, Matsuda K, Nakashima M, Kitawaki J.
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Journal Title
Eur J Obstet Gynecol Reprod Biol.
Volume: 264
Pages: 103-116
DOI
Related Report
Peer Reviewed
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[Journal Article] An axonemal alteration in apical endometria of human adenomyosis2021
Author(s)
Khan KN, Fujishita A, Suematsu T, Ogawa K, Koshiba A, Mori T, Itoh K, Teramukai S, Matsuda K, Nakashima M, Kitawaki J
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Journal Title
Hum Reprod
Volume: 36(6)
Issue: 6
Pages: 1574-1589
DOI
Related Report
Peer Reviewed
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