Multi-omics analysis on proteins expressed in human endolymphatic san of Meniere's disease

• Project/Area Number: 18K09333
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56050:Otorhinolaryngology-related
• Research Institution: Kindai University
• Principal Investigator: DOI Katsumi 近畿大学, 医学部, 教授 (40243224)
• Co-Investigator(Kenkyū-buntansha): 日比野 浩 新潟大学, 医歯学系, 教授 (70314317)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2020: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: メニエール病 / マルチオミックス解析 / 内リンパ水腫 / 聴神経腫瘍 / 内リンパ嚢 / 遅発性内リンパ水腫

Outline of Final Research Achievements

Multi-omics data analysis on proteins expressed in human endolymphatic sac of Meniere's disease and vestibular schwannoma patients was completed. We could identified 881 proteins in human endolymphatic sac. Expression profile of several proteins was quite different between Meniere's disease and vestibular schwanooma. We are now evaluating expression amounts as well as expression patterns and trying to see key-molecules which may cause endolymphatic hydros and Meniere's disease.

Academic Significance and Societal Importance of the Research Achievements

ヒトおよび動物内リンパ嚢のマルチオミックス解析は世界初の試みであり、今後の内耳組織に対するプロテオミクス研究の礎・マイルストーンになる画期的な研究と考えられる。ヒト内リンパ嚢に発現する遺伝子・膜蛋白質の解析は、我々が先行研究において新しい知見を報告したのが最初である。現時点でも、ヒトおよび動物の内リンパ嚢に発現する遺伝子・蛋白質に関する質の高い網羅的解析の報告はなく、その観点から、本研究ならびに我々の先行研究は一定の学術的独自性と創造性を有する。

Research Products

• [Journal Article] Characterisation of the static offset in the travelling wave in the cochlear basal turn (2020)
  • Author(s): T. Ota, F. Nin, S. Choi, S. Muramatsu, S. Sawamura, G. Ogata, M. P. Sato, Kat. Doi, K. Doi, T. Tsuji, S. Kawano, T. Reichenbach, and H. Hibino
  • Journal Title: Pflugers Archiv - European Journal of Physiology Volume: 472 Issue: 5 Pages: 1-11
  • DOI: 10.1007/s00424-020-02373-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterisation of the static offset in the travelling wave in the cochlear basal turn (2020)
  • Author(s): Ota T, Nin F, Choi S, Muramatsu S, Sawamura S, Ogata G, Sato M, Doi K, Doi K, Tsuji T, Kawano S, Reichenbach T, Hibino H
  • Journal Title: Pflugers Archiv European Journal of Physiology Volume: 22 Issue: 1 Pages: 173-207
  • DOI: 10.1007/bf01640316
  • Peer Reviewed / Open Access
• [Journal Article] Prevalence of potential candidates for electric-acoustic stimulation implant in a hearing-impaired population. (2019)
  • Author(s): Saito K, Fujita T, Osaki Y, Koyama H, Shiraishi K, Kobayashi T, Sato M, Seo T, Doi K.
  • Journal Title: Auris Nasus Larynx. Volume: 47 Issue: 2 Pages: 198-202
  • DOI: 10.1016/j.anl.2019.07.006
  • Peer Reviewed / Open Access
• [Journal Article] The prevalence loss of vestibular schwannoma among patients treated as sudden sensorineural hearing (2018)
  • Author(s): Fujita T, Saito K, Kashiwagi N, Sato M, Seo T, Doi K
  • Journal Title: Auris Nasus Larynx Volume: 46 Issue: 1 Pages: 78-82
  • DOI: 10.1016/j.anl.2019.04.006
  • Peer Reviewed / Open Access
• [Journal Article] Recent and Frequent Vertigo Attacks Produce Negative Findings on Furosemide-Loading Vestibular Evoked Myogenic Potential Testing in Meniere's Disease (2018)
  • Author(s): Seo T, Shiraishi K, Kobayashi T, Fujita T, Saito K, Doi K
  • Journal Title: Frontiers in Neurology Volume: in press Pages: 0-0
  • DOI: 10.3389/fneur.2019.00047
  • Peer Reviewed / Open Access
• [Journal Article] Sound Change Integration Error: An Explanatory Model of Tinnitus (2018)
  • Author(s): Noda Kazuhiro、Kitahara Tadashi、Doi Katsumi
  • Journal Title: Frontiers in Neuroscience Volume: 12 Pages: 831-831
  • DOI: 10.3389/fnins.2018.00831
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 内リンパ水腫に対するフロセミド負荷cVEMPと内耳造影MRIの相関性について (2020)
  • Author(s): 白石 功, 岩元 秀輔, 小林 孝光, 土井 勝美
  • Organizer: 日本めまい平衡医学会
• [Presentation] VSB surgery for conductive and mixed hearing loss - the most sensitive predictable factor for speech perception with VSB - (2019)
  • Author(s): Doi K
  • Organizer: the 56th Inner Ear Biology Workshop
• [Presentation] VSB surgery for conductive & mixed HL (2018)
  • Author(s): Doi K
  • Organizer: the 31st Politzer Society Meeting (Spain)
  • Int'l Joint Research / Invited
• [Presentation] Preservation of vestibular function after cochlear implantation with round window approach (RWA) (2018)
  • Author(s): Doi K
  • Organizer: the 30th Barony Society Meeting (Sweden)
  • Int'l Joint Research
• [Presentation] 3D-CT analysis of the FMT angle to RW/OW after VSB surgery - its correlation to the hearing sensitivity - (2018)
  • Author(s): Doi k
  • Organizer: the 55th Inner Ear Biology Meeting (Germany)
  • Int'l Joint Research
• [Presentation] VSB surgery for conductive & mixed HL (2018)
  • Author(s): Doi K
  • Organizer: the 6th East Asian Symposium on Otology (Korea)
  • Int'l Joint Research / Invited
• [Presentation] 小児の難聴と診断 (2018)
  • Author(s): 土井 勝美
  • Organizer: 第119回日本耳鼻咽喉科学会
  • Invited