| Project/Area Number |
18K09349
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56050:Otorhinolaryngology-related
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
熊井 良彦 長崎大学, 医歯薬学総合研究科(医学系), 教授 (00555774)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | 筋線維芽細胞 / 頚部瘢痕 / αSMA / 骨格筋線維化 / PGE2 / 頸部瘢痕 / 声帯瘢痕 / フェレット瘢痕声帯モデル / 筋繊維芽細胞の分離培養 / フェレット / 瘢痕声帯 / プロスタグランジンE2 |
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| Outline of Final Research Achievements |
The neck of the mouse was irradiated with 24 Gy or 72 Gy. Once, and after 10 days, 1 month, and 3 months, whole tissue of the strap muscle (which is the key for healthy swallow) was collected and PCR was performed. With the measurement of mRNA level of various fibrosis markers such as αSMA, TGFb, Collagen I and with Masson Trichrome staining, it was found that the obvious mouse fibrotic changes in strap muscle can be detected with a single irradiation of 72 Gy. This observation validated that strap muscle irradiated with single shot of 72Gy as a model of radiation induced fibrosis in neck muscle related to swallowing function.
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| Academic Significance and Societal Importance of the Research Achievements |
今後頸部放射線照射に伴う線維化（瘢痕組織）に対する新規治療法開発を検討するうえでの、的確で有用な放射線誘発頸部線維化マウスモデルの作製ができた。
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