Functional analysis of adipose-derived stem cells by in vivo lineage tracing and development of novel regenerative therapies

• Project/Area Number: 18K09490
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Jichi Medical University
• Principal Investigator: Sunaga Ataru 自治医科大学, 医学部, 講師 (00406117)
• Co-Investigator(Kenkyū-buntansha): 吉村 浩太郎 自治医科大学, 医学部, 教授 (60210762) ; 宇田 宏一 自治医科大学, 医学部, 准教授 (20337306)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脂肪由来幹細胞 / 系譜追跡 / 再生医療

Outline of Final Research Achievements

We generated PAG mice in which cells derived from Prx1-expressing cells permanently express tdTomato and type I collagen-expressing cells express EGFP by crossbreeding three types of genetically modified mice. ADSC sheets) were prepared from PAG mice. After establishing a mouse model of tendon injury, tdT-ADSC sheets were transplanted into the injured area of the tendon and the transplanted tendon was observed by fluorescent immunostaining. The transplanted tdT-ADSC sheets were observed by fluorescence immunostaining. tdTomato-positive GFP-positive cells were found in the repaired tendon, indicating that the transplanted tdTomato-positive ADSCs contributed to collagen production in the tendon repair process.

Academic Significance and Societal Importance of the Research Achievements

脂肪由来幹細胞による腱の再生治療の可能性が示された。