Establishment of induction method from iPS cells to odontogenic cells
Project/Area Number |
18K09511
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 57010:Oral biological science-related
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Research Institution | Showa University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
上條 竜太郎 昭和大学, 歯学部, 教授 (70233939)
|
Project Period (FY) |
2018-04-01 – 2021-03-31
|
Project Status |
Completed (Fiscal Year 2020)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | iPS細胞 / エナメル芽細胞 / 象牙芽細胞 / 神経堤由来細胞 / 上皮-間葉相互作用 |
Outline of Final Research Achievements |
We investigated techniques for inducing iPS cells to dental epithelial cells or dental mesenchymal cells. 1) iPS cells were induced into neural crest-derived cells by EGF and FGF-2, and then into odontoblast-like cells by BMP-2. These inductions increased the gene expression of DSPP and RUNX2. 2) iPS cells were induced into keratinocytes by retinoic acid and BMP-4, and then into ameloblast-like cells by FGF-8 and Shh. These inductions increased the gene expression of AMBN and AMELX. These results demonstrated that iPS cells could be guided differentiation toward odontogenic lineage via regulating the signaling of tooth development, without odontogenic cells.
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Academic Significance and Societal Importance of the Research Achievements |
iPS細胞から歯原性細胞のサポート無しにエナメル芽細胞へ誘導することは、不明な点が多いエナメル芽細胞分化を解き明かす一助になる。iPS細胞から誘導した歯原性上皮細胞と歯原性間葉細胞の上皮‐間葉相互作用による歯原性細胞に依存しない歯胚形成は、歯を失った高齢者などに対応できる歯胚再生法として有望と考える。
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Zoledronate promotes inflammatory cytokine expression in human CD14-positive monocytes among peripheral mononuclear cells in the presence of γδ T cells.2021
Author(s)
Takimoto R, Suzawa T, Yamada A, Sasa K, Miyamoto Y, Yoshimura K, Sasama Y, Tanaka M, Kinoshita M, Ikezaki K, Ichikawa M, Yamamoto M, Shirota T, Kamijo R
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Journal Title
Immunology
Volume: 162(3)
Issue: 3
Pages: 306-313
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Functional analysis of PTH1R variants found in primary failure of eruption.2020
Author(s)
Izumida E, Suzawa T, Miyamoto Y, Yamada A, Takimoto R, Otsu M, Saito T, Yamaguchi T, Nishimura K, Ohtaka M, Nakanishi M, Yoshimura K, Sasa K, Takimoto R, Uyama R, Shirota T, Maki K, Kamijo R.
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Journal Title
Journal of Dental Research
Volume: 99
Issue: 4
Pages: 429-436
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Dental implant care and trouble among dependent patients based on the questionnaire survey among Japanese dental practitioners.2020
Author(s)
Sato Y, Koyama S, Ohkubo C, Ogura S, Kamijo R, Sato S, Aida J, Izumi Y, Atsumi M, Isobe A, Baba S, Ikumi N, Watanabe F
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Journal Title
BMC Oral Health
Volume: 20
Issue: 1
Pages: 335-341
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effects of lipid metabolism on mouse incisor dentinogenesis.2020
Author(s)
Kurotaki, Y., Sakai, N., Miyazaki, T., Hosonuma, M., Sato, Y., Karakawa, A., Chatani, M., Myers, M., Suzawa, T., Negishi-Koga, T., Kamijo, R., Miyazaki, A., Maruoka, Y., Takami, M.
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Journal Title
Scientific Reports
Volume: 10
Issue: 1
Pages: 5102-5102
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Prospects of neural crest-derived cells from oral and dentofacial tissues for application in regenerative medicine.2020
Author(s)
Suzawa T, Yoshida H, Takahashi M, Itose M, Takimoto R, Sasama Y, Tanaka M, Ikezaki K, Shirora T, Maki K, Kamijo R.
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Journal Title
Oral Science International
Volume: 17
Related Report
Peer Reviewed / Int'l Joint Research
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