Study of the effect of serotonin in the salivary glands on salivary secretion: using salivary gland microdialysis.

• Project/Area Number: 18K09514
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57010:Oral biological science-related
• Research Institution: Tokai University
• Principal Investigator: Yoshikawa Masanobu 東海大学, 医学部, 准教授 (90276791)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: マイクロダイアリシス / 唾液腺 / 神経伝達物質 / 唾液分泌 / アセチルコリン / 中枢神経 / 抗うつ薬 / セロトニン / 唾液腺マイクロダイアリシス

Outline of Final Research Achievements

To date, it has been difficult to directly monitor nerve activity in the salivary glands. We applied an in vivo microdialysis method to the salivary gland to monitor multiple neurotransmitters such as acetylcholine, norepinephrine, serotonin, and amino acid simultaneously in the submandibular gland of rats. We applied in vivo microdialysis to salivary glands and simultaneously monitored multiple neurotransmitters such as acetylcholine, norepinephrine, serotonin, and amino acid in the submandibular gland of rats to analyze the effects of the release of various neurotransmitters in the salivary glands on salivary secretion.The results revealed that the amount of neurotransmitter release in the dialysate reflects neural activity within the salivary glands.

Academic Significance and Societal Importance of the Research Achievements

唾液腺in vivoマイクロダイアリシス法が唾液腺内の副交感神経活動の変化を検出するための強力なツールになることが明らかとなった。唾液腺in vivoマイクロダイアリシス法を用いることで唾液腺内神経系を介する唾液腺機能調節機構が明らかにされれば、新たな口腔乾燥症治療薬が開発されることが期待される。

Research Products

• [Journal Article] Free D-amino acids in salivary gland in rat (2022)
  • Author(s): Yoshikawa M, Kan T, Shirose K, Watanabe M, Matsuda M, Ito K, Kawaguchi M
  • Journal Title: Biology Volume: 11 Issue: 3 Pages: 390-405
  • DOI: 10.3390/biology11030390
  • Peer Reviewed / Open Access
• [Journal Article] In Vivo Monitoring of Acetylcholine Release from Nerve Endings in Salivary Gland (2021)
  • Author(s): Masanobu Yoshikawa and Mitsuru Kawaguchi
  • Journal Title: Biology Volume: 10 Issue: 5 Pages: 351-351
  • DOI: 10.3390/biology10050351
  • Peer Reviewed / Open Access
• [Presentation] ラット顎下腺内副交感神経終末からのアセチルコリン遊離に対するD-セリンの作用 (2019)
  • Author(s): 吉川正信、大久保みぎわ、川口充
  • Organizer: 第64回日本唾液腺学会総会