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Potential role of interleukin-29 in oral mucosal defense against viruses

Research Project

Project/Area Number 18K09548
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57020:Oral pathobiological science-related
Research InstitutionNational Center for Geriatrics and Gerontology

Principal Investigator

Shikama Yosuke  国立研究開発法人国立長寿医療研究センター, 口腔疾患研究部, 副部長 (10588908)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords口腔粘膜 / 自然免疫 / ウイルス感染 / インターフェロン / ウイルス
Outline of Final Research Achievements

Interferon (IFN) lambda-1 (IFN-l1), a type III IFN, exhibits type I IFN-like antiviral activity. In contrast to ubiquitously expressed type I IFN receptors, IFN-l receptor 1 (IFN-lR1) is mainly expressed on epithelial cells. The regulation of type III IFN receptor expression and its functions in the oral mucosa remain unclear. We herein showed the expression of IFN-lR1 in human gingival keratinocytes. The expression of IL-6 and IL-8 in human primary gingival keratinocytes (HGK) was significantly higher following treatments with either type I IFN (IFN-b) or type II IFN (IFN-g) than with IFN-l1. However, the IFN-l1 treatment strongly induced toll-like receptor (TLR) 3 and retinoic acid-inducible gene I (RIG-I), which mainly recognize viral nucleic acids, via the STAT-1-mediated pathway. Furthermore, a stimulation with a RIG-I or TLR3 agonist promoted the production of IL-6, IL-8, and IFN-b in HGK, which was significantly enhanced by a pretreatment with IFN-l1.

Academic Significance and Societal Importance of the Research Achievements

ウイルスに関する研究は非常に幅広く行われているが、口腔粘膜における抗ウイルス能の増強、賦活作用に着目した検討はほどんど行われていない。歯科領域疾患の中で、ウイルス感染に起因する疾患の予防、治療法開発は臨床上非常に重要であると考えられる。本研究はIL-29が口腔粘膜を介したウイルス感染予防法として臨床応用出来る可能性を示した、意義深い研究である。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (29 results)

All 2022 2021 2020 2019 2018 Other

All Journal Article (13 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 12 results,  Open Access: 8 results) Presentation (11 results) (of which Int'l Joint Research: 1 results) Remarks (5 results)

  • [Journal Article] The Priming Potential of Interferon Lambda-1 for Antiviral Defense in the Oral Mucosa2022

    • Author(s)
      Shikama Yosuke、Kurosawa Mie、Furukawa Masae、Kudo Yasusei、Ishimaru Naozumi、Matsushita Kenji
    • Journal Title

      Inflammation

      Volume: - Issue: 3 Pages: 1-14

    • DOI

      10.1007/s10753-022-01624-1

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chemokines Up-Regulated in Epithelial Cells Control Senescence-Associated T Cell Accumulation in Salivary Glands of Aged and Sj?gren’s Syndrome Model Mice2021

    • Author(s)
      Kurosawa Mie、Shikama Yosuke、Furukawa Masae、Arakaki Rieko、Ishimaru Naozumi、Matsushita Kenji
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 5 Pages: 2302-2302

    • DOI

      10.3390/ijms22052302

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of green propolis extracts on experimental aged gingival irritation in vivo and in vitro2021

    • Author(s)
      Furukawa Masae、Wang Jingshu、Kurosawa Mie、Ogiso Noboru、Shikama Yosuke、Kanekura Takuro、Matsushita Kenji
    • Journal Title

      Journal of Oral Biosciences

      Volume: 63 Issue: 1 Pages: 58-65

    • DOI

      10.1016/j.job.2020.12.003

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Basic Studies on the Mechanism, Prevention, and Treatment of Osteonecrosis of the Jaw Induced by Bisphosphonates2020

    • Author(s)
      Endo Yasuo、Funayama Hiromi、Yamaguchi Kouji、Monma Yuko、Yu Zhiqian、Deng Xue、Oizumi Takefumi、Shikama Yosuke、Tanaka Yukinori、Okada Satoshi、Kim Siyoung、Kiyama Tomomi、Bando Kanan、Shima Kazuhiro、Suzuki Hikari、Takahashi Tetsu
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 140 Issue: 1 Pages: 63-79

    • DOI

      10.1248/yakushi.19-00125

    • NAID

      130007778763

    • ISSN
      0031-6903, 1347-5231
    • Year and Date
      2020-01-01
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dietary diversity and characteristics of lifestyle and awareness of health in Japanese workers : a cross-sectional study2020

    • Author(s)
      Nakamoto Mariko、Yun Yuna、Omine Mariko、Mori Sarasa、Shuto Emi、Nakamoto Akiko、Hata Akiko、Aki Nanako、Shikama Yosuke、Bando Yukiko、Ichihara Takako、Minagawa Takako、Tamura Ayako、Kuwamura Yumi、Funaki Makoto、Sakai Tohru
    • Journal Title

      The Journal of Medical Investigation

      Volume: 67 Issue: 3.4 Pages: 255-264

    • DOI

      10.2152/jmi.67.255

    • NAID

      130007936871

    • ISSN
      1343-1420, 1349-6867
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Imaging of muscle activity‐induced morphometric changes in fibril network of myofascia by two‐photon microscopy2020

    • Author(s)
      Chaweewannakorn Chayanit、Harada Takashi、Nyasha Mazvita R.、Koide Masashi、Shikama Yosuke、Hagiwara Yoshihiro、Sasaki Keiichi、Kanzaki Makoto、Tsuchiya Masahiro
    • Journal Title

      Journal of Anatomy

      Volume: 238 Issue: 3 Pages: 515-526

    • DOI

      10.1111/joa.13339

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] High concentration of glucose induces filaggrin-1 expression through AP-1 in skin keratinocytes2020

    • Author(s)
      Masae Furukawa, Kiyoko Yamada, Mie Kurosawa, Yosuke Shikama, Takuro Kanekura and Kenji Matsushita
    • Journal Title

      Journal of Dermatological Science

      Volume: - Issue: 2 Pages: 137-140

    • DOI

      10.1016/j.jdermsci.2020.04.002

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Periodontal Disease and Periodontal Disease-Related Bacteria Involved in the Pathogenesis of Alzheimer’s Disease2020

    • Author(s)
      Matsushita Kenji、Yamada-Furukawa Masae、Kurosawa Mie、Shikama Yosuke
    • Journal Title

      Journal of Inflammation Research

      Volume: Volume 13 Pages: 275-283

    • DOI

      10.2147/jir.s255309

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Associations of Dietary Diversity With Allergic Diseases in Japanese Workers: A Cross-Sectional Study2019

    • Author(s)
      Nakamoto M, Omine M, Yun Y, Shuto E, Nakamoto A, Hata A, Aki N, Shikama Y, Bando Y, Ichihara T, Minamigawa T, Tamura A, Kuwamura Y, Funaki M, Sakai T.
    • Journal Title

      Asia Pac J Clin Nutr

      Volume: 28 Pages: 857-869

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of adiponectin in age-related increases in tear production in mice2019

    • Author(s)
      Shikama Yosuke、Kurosawa Mie、Furukawa Masae、Ishimaru Naozumi、Matsushita Kenji
    • Journal Title

      Aging

      Volume: 11 Issue: 19 Pages: 8329-8346

    • DOI

      10.18632/aging.102322

    • NAID

      120007146099

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] IL-29 は口腔粘膜上皮細胞においてRIG-IおよびIFI-16発現誘導を介し 抗ウイルス活性を増強する2019

    • Author(s)
      四釜洋介、黒澤実愛、松下健二
    • Journal Title

      エンドトキシン・自然免疫研究22 エンドトキシン・自然免疫研究の新たな可能性を求めて

      Volume: 22 Pages: 40-42

    • Related Report
      2019 Research-status Report
  • [Journal Article] Soy product and isoflavone intake associations with allergic diseases in Japanese workers: rhinitis, dermatitis and asthma.2018

    • Author(s)
      Nakamoto M, Shuto E, Nakamoto A, Hata A, Aki N, Shikama Y, Bando Y, Ichihara T, Minagawa T, Tamura A, Kuwamura Y, Funaki M, Sakai T.
    • Journal Title

      Asia Pacific Journal of Clinical Nutrition

      Volume: 27 Pages: 1277-1285

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Free fatty acids may be involved in the pathogenesis of oral-related and cardiovascular diseases2018

    • Author(s)
      Shikama Y.
    • Journal Title

      Journal of Oral Biosciences

      Volume: 60 Pages: 65-69

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] III型interferon(IFN)であるinterleukin(IL)-29の口腔粘膜における抗ウイルス応答増強作用2021

    • Author(s)
      四釜洋介、古川匡恵、松下健二
    • Organizer
      第64回秋季日本歯周病学会学術大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 加齢に伴う涙液分泌の変化:ヒトとマウスの相違およびアディポネクチンの関連性2021

    • Author(s)
      四釜洋介
    • Organizer
      第74回日本臨床眼科学会
    • Related Report
      2020 Research-status Report
  • [Presentation] 老化関連T細胞および唾液腺上皮の細胞老化が口腔乾燥症の病態形成に関与する可能性2021

    • Author(s)
      黒澤実愛、古川匡恵、松下健二、四釜洋介
    • Organizer
      第62回歯科基礎医学会学術大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 老齢マウスにおけるアディポネクチンと涙液分泌の関連性2021

    • Author(s)
      四釜洋介、黒澤実愛、古川匡恵、松下健二
    • Organizer
      第43回日本基礎老化学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Senescence-associated T-lymphocytes accumulate in the submandibular glands of aged mice2019

    • Author(s)
      Kurosawa Mie, Furukawa Masae, Matsushita Kenji, Shikama Yosuke
    • Organizer
      97th IADR General Session
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] エストロゲンの欠乏は唾液腺上皮細胞にCXCL13産生を誘導し、老化関連T細胞集積を促進する2019

    • Author(s)
      四釜洋介、黒澤実愛、古川匡恵、松下健二
    • Organizer
      第42回日本基礎老化学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 免疫老化及び細胞老化による唾液腺へのSA-T細胞集積メカニズムの解析2019

    • Author(s)
      黒澤実愛、古川匡恵、松下健二、四釜洋介
    • Organizer
      第38回分子病理学研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] 唾液腺上皮の細胞老化及び免疫老化が唾液腺機能に与える影響:老齢マウスおよびモデルマウスを用いた解析2019

    • Author(s)
      黒澤実愛、古川匡恵、松下健二、四釜洋介
    • Organizer
      第28回日本シェーグレン症候群学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] 加齢および脂質代謝異常によるドライマウス発症機序とその病態解明2018

    • Author(s)
      四釜洋介
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 口腔粘膜におけるIL-29の抗真菌および抗ウイルス作用:臨床応用の可能性2018

    • Author(s)
      四釜洋介、黒澤実愛、山田(古川)匡恵、松下健二
    • Organizer
      第61回秋季日本歯周病学会学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] IL-29は口腔粘膜上皮細胞においてRIG-I発現誘導を介し抗ウイルス活性を増強する2018

    • Author(s)
      四釜洋介、黒澤実愛、松下健二
    • Organizer
      第24回日本エンドトキシン・自然免疫研究会
    • Related Report
      2018 Research-status Report
  • [Remarks] 四釜洋介HP

    • URL

      http://shikamax.net/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 口腔疾患研究部HP

    • URL

      https://www.ncgg.go.jp/ri/lab/gero/department/oralscience/home.html

    • Related Report
      2021 Annual Research Report
  • [Remarks] 国立長寿医療研究センター口腔疾患研究部ホームページ

    • URL

      https://www.ncgg.go.jp/research/department/odr/index.html

    • Related Report
      2019 Research-status Report
  • [Remarks] 四釜洋介ホームページ

    • URL

      http://shikamax.net/

    • Related Report
      2019 Research-status Report 2018 Research-status Report
  • [Remarks] 国立長寿医療研究センター口腔疾患研究部ホームページ

    • URL

      http://www.ncgg.go.jp/research/department/odr/index.html

    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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