Study on pathogenicity of Rothia mucilaginosa and development of the anti-infective therapy

• Project/Area Number: 18K09613
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Osaka Dental University
• Principal Investigator: Maeda Hiroshi 大阪歯科大学, 歯学部, 教授 (00274001)
• Co-Investigator(Kenkyū-buntansha): 辻 則正 大阪歯科大学, 歯学部, 助教 (30454565) ; 高柴 正悟 岡山大学, 医歯薬学総合研究科, 教授 (50226768) ; 曽我 賢彦 岡山大学, 大学病院, 准教授 (70509489)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: Rothia / 日和見感染 / 根管 / 易感染性宿主 / 感染根管 / MMP / Rothia mucilaginosa / Rothia aeria / Rothia dentocariosa / 病巣感染 / 白血病 / 根尖性歯周炎 / 敗血症

Outline of Final Research Achievements

Distribution of Rothia species (Rothia mucilaginosa, Rothia aeria and Rothia dentocariosa), known to be opportunistic pathogens, in root canals was investigated. The Rothia spp. were frequently detected in root canals among Japanese population. The presence of Rothia spp. showed correlations with the inflammatory conditions at the apical lesions. The cell components of Rothia demonstrated high level of matrix metalloprotease activity as a potential virulence factor.

Academic Significance and Societal Importance of the Research Achievements

口腔内に生息するRothia種は宿主の抵抗性が低下した場合に重篤な全身感染症を引き起こす場合がある。本細菌の口腔内分布を調べることは感染対策上重要である。また、病原因子を解明することは、将来的な診断と治療法の開発に結び付く可能性がある。根管は歯槽骨内と直結する空間であり、本研究結果から根管がRothiaの全身への感染ルートとなる可能性が示された。今後、根管内のRothiaへの感染対策を実施することで、全身感染症の発症を抑制できる可能性がある。

Research Products

• [Journal Article] Rapid and simple detection of <i>vanA </i>and <i>vanB </i>by the loop­mediated isothermal amplification (LAMP) method (2020)
  • Author(s): Hideaki Ikenaga, Hiroshi Maeda, Yoki Hirakawa, Yuki Aso, Norimasa Tsuji, Ikuo Nishikawa, Sadaomi Sugimoto, Ayano Miyamoto, Takeshi Inamoto, Hitomi Nakama, Muneyasu Shida and Masataka Yoshikawa
  • Journal Title: Journal of Osaka Dental University Volume: 54 Issue: 2 Pages: 183-191
  • DOI: 10.18905/jodu.54.2_183
  • NAID: 130007975248
  • ISSN: 0475-2058, 2189-6488
  • Year and Date: 2020-10-25
  • Peer Reviewed / Open Access
• [Journal Article] Identification of MMP-like protein from Streptococcus mitis (2020)
  • Author(s): Hiroaki Aso, Hiroshi Maeda, Takayuki Nambu, Toshinori Okinaga, Muneyasu Shida
  • Journal Title: World Journal of Advanced Research and Reviews Volume: 8 Issue: 3 Pages: 154-161
  • DOI: 10.30574/wjarr.2020.8.3.0473
  • Peer Reviewed / Open Access
• [Journal Article] Distribution of Rothia species in root canals in a Japanese population (2019)
  • Author(s): Teramoto Satoshi, Maeda Hiroshi , Tsuji Norimasa, Ikenaga Hideaki, Yoshikawa Masataka
  • Journal Title: World Journal of Advanced Research and Reviews Volume: 4 Issue: 2 Pages: 20-26
  • DOI: 10.30574/wjarr.2019.4.2.0095
  • Peer Reviewed / Open Access
• [Journal Article] Selective growth inhibition of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans by antisense peptide nucleic acids (2019)
  • Author(s): Sugimoto S, Maeda H, Kitamatsu M, Nishikawa I, Shida M
  • Journal Title: Molecular and Cellular probes Volume: 43 Pages: 45-49
  • DOI: 10.1016/j.mcp.2018.11.006
  • Peer Reviewed / Open Access
• [Presentation] 日本人の感染根管におけるRothia菌種の分布調査ならびにR. mucilaginosaの定量検出 (2020)
  • Author(s): 麻生由樹，至田宗泰，西川郁夫，池永英彰，稲本雄之，辻 則正，仲間ひとみ，杉本貞臣，麻生浩章，前田博史
  • Organizer: 日本歯科保存学会2020年度春季学術大会（第152回）
• [Presentation] 日本人の感染根管内におけるRothia菌種の分布状況 (2019)
  • Author(s): 寺本賢史，前田博史，好川正孝，池永英彰，辻 則正
  • Organizer: 日本歯科保存学会2019年度秋季学術大会（第151回）