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Attempt to estimate the time of alcohol consumption based on ethanol exposure-induced changes in microRNA expression

Research Project

Project/Area Number 18K10128
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 58040:Forensics medicine-related
Research InstitutionKochi University

Principal Investigator

Akinori Nakanishi  高知大学, 教育研究部医療学系連携医学部門, 助教 (10217763)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords飲酒証明 / マイクロRNA / アルコール / 飲酒
Outline of Final Research Achievements

In forensic practice, determining whether the autopsied person drank alcohol is an important test. However, depending on the time between the time when the alcohol was consumed and when the samples were collected, the ethanol (EtOH) concentration may have decreased markedly from the body. Therefore, there is a need for a method to establish whether someone has consumed alcohol that is not based on EtOH in the body. In this study we used multiple EtOH-responsive microRNA(E-miR) and investigated the extent to which they are expressed in individual organs of mice administered EtOH to determine their utility as indices of the proof of drinking. Because E-miRs (miR-9, miR-15b, miR-21 and miR-335) showed different responses to EtOH exposure in individual organs and it also revealed differences in depending on the amount of EtOH administered, by analyzing the multiple miRs in multiple organs, it may be possible to devise a novel method to prove alcohol consumption that does not rely on EtOH.

Academic Significance and Societal Importance of the Research Achievements

法医実務では、解剖症例の飲酒の有無を明らかにすることは重要な検査項目であるが、飲酒してから検査試料を採取するまで相当の時間が経過した場合、既に体内エタノール(EtOH)濃度の低下又は消失により飲酒の証明が困難な事例も発生する。そのような事態に対処するため、体内EtOHを指標としない飲酒証明法の開発は法医学上非常に有用である。本研究の結果、EtOH曝露により発現量が顕著に増減する4種類のmicroRNAを指標とした全く新しい分子生物学的手法による飲酒証明の可能性が期待できると思われた。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2021 2020 2019

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (4 results)

  • [Journal Article] microRNAを指標としたエタノール摂取時期推定に向けた基礎的検討2020

    • Author(s)
      中西祥徳、道家章斗、西村拡起、古宮淳一
    • Journal Title

      DNA多型

      Volume: 28 Pages: 70-73

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] microRNAを指標としたエタノール摂取時期推定に向けた基礎的検討2020

    • Author(s)
      中西祥徳、道家章斗、西村拡起、古宮淳一
    • Journal Title

      DNA多型 Vol.28

      Volume: 印刷中

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] エタノール反応性microRNAのマウス臓器中発現量に及ぼすエタノール濃度の短時間影響2021

    • Author(s)
      中西祥徳、道家章斗、西村拡起、古宮淳一
    • Organizer
      第105次日本法医学会学術全国集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] エタノール投与後24時間までのマウス諸臓器中マイクロRNAの変動2020

    • Author(s)
      中西祥徳、道家章斗、西村拡起、古宮淳一
    • Organizer
      第104次日本法医学会学術全国集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] microRNAを指標としたエタノール摂取時期推定に向けた基礎的検討2019

    • Author(s)
      中西祥徳、道家章斗、西村拡起、古宮淳一
    • Organizer
      日本DNA多型学会第28回学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] エタノール投与によるマウス臓器中microRNA発現量の経時的変化2019

    • Author(s)
      中西祥徳、道家章斗、西村拡起、古宮淳一
    • Organizer
      第103次日本法医学会学術全国集会
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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