| Project/Area Number |
18K10137
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 58040:Forensics medicine-related
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
神田 芳郎 久留米大学, 医学部, 教授 (90231307)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ABO式血液型 / 転写調節 / FUT1 / ゲノム編集 / 種間変異 / CRISPR/Cas9 / Alu配列 / 分子進化 / 転写制御 / 比較生物学 |
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| Outline of Final Research Achievements |
ABO antigens are histo-blood group antigens and these antigens are detected only on human and anthropoid apes, that is, ABO are not blood group antigens in other mammals. In this research project, to elucidate the mechanism of perhaps evolutionally acquired some species-specific tissue distribution pattern, we focused on the transcriptional regulation of the FUT1 that regulated the expression of the H and therefore A and B antigens in erythroid lineage. We tried to knock out an Alu element, that is one of specific repetitive sequences to human and anthropoid apes of those erythrocytes carried no ABO antigens, resided in the second one of three FUT1 promoters by CRISPR-Cas9 system in the erythroleukemia cell lines. However, we can not any Alu element knock-out clones so far.
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| Academic Significance and Societal Importance of the Research Achievements |
ABO式血液型は、人で最初に見いだされたメンデル遺伝形質であり、輸血医学や法医学の分野で重要な役割を果たしてきた最も代表的な血液型である。この血液型が血液（赤血球膜）に認められる動物が、実は自分たちヒトを含む一部の動物種に限られているという現象は非常に興味深く、そのメカニズムを解明することが出来れば、学術的にも社会的にも意義のある研究成果であると考える。しかしながら、今回用いた実験的なアプローチでは残念ながら解明することが出来なかった。
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