Studies on genes involved in skeletal muscle hypotrophy in stroke-prone spontaneously hypertensive rats

• Project/Area Number: 18K11116
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 59040:Nutrition science and health science-related
• Research Institution: Kindai University
• Principal Investigator: Takao INOUE 近畿大学, 医学部, 助教 (00441006)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 骨格筋 / 高血圧 / 脳卒中 / マイオカイン / 骨格筋発育低下 / 生活習慣病

Outline of Final Research Achievements

Stroke-prone spontaneously hypertensive rats (SHRSP) have lower weight than normotensive rats (WKY). The results obtained from investigating for skeletal muscle showed that slow muscle, but not fast muscle had specific lower weight in SHRSP. Comprehensive gene expression analysis for soleus, slow muscle, clarified protein involved in protein degradation highly expressed in slow muscle of SHRSP. Further analysis for upstream of the protein showed that the upstream protein which expresses mainly in fast muscle tended to highly express in slow muscle of SHRSP. This indicated that ectopic expression in slow muscle of the upstream protein might be involved in slow muscle specific hypotrophy in SHRSP.

Academic Significance and Societal Importance of the Research Achievements

高血圧症は、超高齢化社会において多くの割合を占める疾患であり、脳卒中を始めとする様々な疾患の原因となる。脳卒中の発症は、自身の力で生活することができる「健康寿命」を短くする。骨格筋は、運動機能の維持だけではなく、身体の恒常性の維持に重要であることが知られている。従って、本研究における脳卒中発症ラットにおける骨格筋の発育異常を改善できれば、高血圧症のみならず、脳卒中の病態改善に役立つ可能性がある。

Research Products

• [Journal Article] ECTOPIC TWEAKR EXPRESSION IN TYPE I FIBER OF STROKE-PRONE SPONTANEOUSLY HYPERTENSIVE RATS IS RELATED TO SLOW MUSCLE-SPECIFIC HYPOTROPHY THROUGH MURF1 OVEREXPRESSION (2021)
  • Author(s): Inoue Takao、Hagiyama Man、Maenishi Osamu、Kimura Masatomo、Mizuguchi Nobuyuki、Mine Yoshihiro、Kimura Ryuichiro、Chikugo Takaaki、Itoh Tatsuki、Satou Takao、Ito Akihiko
  • Journal Title: Journal of Hypertension Volume: 39 Issue: Supplement 1 Pages: e254-e254
  • DOI: 10.1097/01.hjh.0000747352.39323.e9
  • Peer Reviewed / Open Access
• [Journal Article] Cell Adhesion Molecule 1 Contributes to Cell Survival in Crowded Epithelial Monolayers (2020)
  • Author(s): Hagiyama M、Kimura R、Yoneshige A、Inoue T、Otani T、Ito A
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Issue: 11 Pages: 4123-4123
  • DOI: 10.3390/ijms21114123
  • Peer Reviewed / Open Access
• [Journal Article] Ectopic TWEAKR expression in type I fiber of stroke-prone spontaneously hypertensive rats is related to slow muscle-specific hypotrophy. (2019)
  • Author(s): Inoue T, Hagiyama M, Maenishi O, Kimura M, Mizuguchi N, Mine Y, Kimura R, Chikugo T, Itoh T, Satou T, Ito A
  • Journal Title: Life Sciences Volume: 237 Pages: 116919-116919
  • DOI: 10.1016/j.lfs.2019.116919
  • Peer Reviewed
• [Journal Article] Urinary cell adhesion molecule 1 is a novel biomarker that links tubulointerstitial damage to glomerular filtration rates in chronic kidney disease. (2019)
  • Author(s): Hagiyama M, Nakatani Y, Takashima Y, Kato T, Inoue T, Kimura R, Otani T, Sato Y, Mori H, Arima S, Ito A
  • Journal Title: Frontiers in Cell and Development Biology Volume: 7 Pages: 111-111
  • DOI: 10.3389/fcell.2019.00111
  • Peer Reviewed / Open Access
• [Journal Article] Progression of pulmonary emphysema and continued increase in ectodomain shedding of cell adhesion molecule 1 after cessation of cigarette smoke exposure in mice. (2018)
  • Author(s): Ri A, Hagiyama M, Inoue T, Yoneshige A, Kimura R, Murakami Y, Ito A.
  • Journal Title: Front Cell Dev Biol Volume: 6 Pages: 52-52
  • DOI: 10.3389/fcell.2018.00052
  • Peer Reviewed / Open Access
• [Presentation] ECTOPIC TWEAKR EXPRESSION IN TYPE I FIBER OF STROKE-PRONE SPONTANEOUSLY HYPERTENSIVE RATS IS RELATED TO SLOW MUSCLE-SPECIFIC HYPOTROPHY THROUGH MURF1 OVEREXPRESSION (2021)
  • Author(s): Takao Inoue, Man Hagiyama, Osamu Maenishi, Masatomo Kimura, Nobuyuki Mizuguchi, Yoshihiro Mine, Ryuichiro Kimura, Takaaki Chikugo, Tatsuki Itoh, Takao Satou, Akihiko Ito
  • Organizer: International Society of Hypertension
  • Int'l Joint Research
• [Presentation] 心筋・骨格筋の異常から探るSHRSPの病態改善への糸口 (2020)
  • Author(s): 井上敬夫
  • Organizer: 高血圧関連疾患モデル学会
  • Invited
• [Presentation] 発育低下のみられる骨格筋におけるTNF関連受容体の発現異常 (2020)
  • Author(s): 1.井上 敬夫、萩山 満、前西 修、木村 雅友、水口 信行、峯 嘉宏、筑後 孝章、伊藤 龍生、佐藤 隆夫、伊藤 彰彦
  • Organizer: 日本農芸化学会 2020年度大会
• [Presentation] 遅筋特異的発育異常におけるMuRF1の発現上昇と上流因子の解析 (2019)
  • Author(s): 2.井上敬夫、前西修、木村雅友、萩山満、水口信行、峯嘉宏、筑後孝章、伊藤龍生、佐藤隆夫、伊藤彰彦
  • Organizer: 第5回 日本筋学会
• [Presentation] 自然な脱毛が見られるメスラットを用いた、女性型脱毛の組織学的検討について (2019)
  • Author(s): 3.辻 ひかり、豊田 啓士、池上 侑希、蒲 尚子、松田 邦子、井上 敬夫、水口 信行、佐藤 隆夫、伊藤 龍生
  • Organizer: 第73回 日本栄養・食糧学会
• [Presentation] 脳卒中発症ラットSHRSPの遅筋特異的発育低下におけるMuRF1の発現上昇 (2019)
  • Author(s): 井上敬夫、萩山満、前西修、水口信行、木村雅友、木村竜一朗、筑後孝章、伊藤龍生、佐藤隆夫、伊藤彰彦
  • Organizer: 日本農芸化学会 2019年度大会
• [Presentation] 病態依存的に発育低下がみられる脳卒中発症ラットSHRSPのヒラメ筋に対する組織学的解析及び遺伝子発現解析 (2018)
  • Author(s): 井上敬夫、萩山満、木村竜一朗、水口信行、前西修、筑後孝章、木村雅友、伊藤龍生、佐藤隆夫、伊藤彰彦
  • Organizer: 日本筋学会 第4回学術集会
• [Presentation] 低形成骨格筋に対する網羅的遺伝子発現解析に基づいたタンパク質分解系の解析 (2018)
  • Author(s): 井上敬夫、萩山満、前西修、水口信行、木村雅友、木村竜一朗、筑後孝章、伊藤龍生、佐藤隆夫、伊藤彰彦
  • Organizer: 第54回 高血圧関連疾患モデル学会学術大会