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Interactome analysis after induction of DNA damage in human cells

Research Project

Project/Area Number 18K11639
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 63020:Radiation influence-related
Research InstitutionNagoya University

Principal Investigator

Kurotani Kenichi  名古屋大学, 生物機能開発利用研究センター, 特任講師 (10402778)

Co-Investigator(Kenkyū-buntansha) 岡 泰由  名古屋大学, 環境医学研究所, 講師 (60762383)
荻 朋男  名古屋大学, 環境医学研究所, 教授 (80508317)
嶋田 繭子  名古屋大学, 環境医学研究所, 技術補佐員 (80623834)
唐田 清伸  名古屋大学, 環境医学研究所, 客員研究者 (90345017)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsDNA損傷応答 / インタラクトーム解析 / 蛋白質間相互作用 / プロテオーム解析
Outline of Final Research Achievements

DNA damage response (DDR) is a crucial response for genome stability. A detailed understanding of their protein-protein interaction networks enables us to know the cellular function of DDR proteins. In this project, we performed the interactome analysis of DDR proteins in human cells using a high resolution mass spectrometry. In the results of our study, we identified several factors that interact with POLR2A/RPB1, the largest subunit of RNA polymerase II, after induction of DNA damage in human cells.

Academic Significance and Societal Importance of the Research Achievements

DNA損傷応答 (DDR)はゲノムの安定化維持機構に極めて重要な役割を持っている。事実、DDR機構の破綻は、成長発達障害、高発がん性、神経変性、早期老化などの様々な疾患の発症に関係していることが知られている。本研究で実施した、DNA損傷誘導後の精密質量分析装置を利用したインタラクトーム解析は、未解明のDDR分子機序の一端を解明したという点において、学術的に優れた成果であったと言える。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (21 results)

All 2020 2019 2018 Other

All Journal Article (7 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 7 results,  Open Access: 6 results) Presentation (11 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Remarks (3 results)

  • [Journal Article] Host-parasite tissue adhesion by a secreted type of β-1,4-glucanase in the parasitic plant Phtheirospermum japonicum2020

    • Author(s)
      Kurotani Ken-ichi、Wakatake Takanori、Ichihashi Yasunori、Okayasu Koji、Sawai Yu、Ogawa Satoshi、Cui Songkui、Suzuki Takamasa、Shirasu Ken、Notaguchi Michitaka
    • Journal Title

      Communications Biology

      Volume: 3 Issue: 1 Pages: 1-11

    • DOI

      10.1038/s42003-020-01143-5

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cell-cell adhesion in plant grafting is facilitated by β-1,4-glucanases2020

    • Author(s)
      Notaguchi, M., Kurotani, K., Sato, Y., Tabata, R., Kawakatsu, Y., Okayasu, K., Sawai, Y., Okada, R., Asahina, M., Ichihashi, Y., Shirasu, K., Suzuki, T., Niwa, M., Higashiyama, T
    • Journal Title

      Science

      Volume: 369 Issue: 6504 Pages: 698-702

    • DOI

      10.1126/science.abc3710

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High-quality sugar production by osgcs1 rice2020

    • Author(s)
      Honma Yujiro、Adhikari Prakash Babu、Kuwata Keiko、Kagenishi Tomoko、Yokawa Ken、Notaguchi Michitaka、Kurotani Kenichi、Toda Erika、Bessho-Uehara Kanako、Liu Xiaoyan、Zhu Shaowei、Wu Xiaoyan、Kasahara Ryushiro D.
    • Journal Title

      Communications Biology

      Volume: 3 Issue: 1 Pages: 617-617

    • DOI

      10.1038/s42003-020-01329-x

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair2020

    • Author(s)
      Nakazawa Y, Hara Y, Oka Y, Komine O, van den Heuvel D, Guo C, Daigaku Y, Isono M, He Y, Shimada M, Kato K, Jia N, Hashimoto S, Kotani Y, Miyoshi Y, Tanaka M, Sobue A, Mitsutake N, Suganami T, Masuda A, Ohno K, Nakada S, Mashimo T, Yamanaka K, Luijsterburg MS, Ogi T
    • Journal Title

      Cell

      Volume: 19 Issue: 6 Pages: 1228-1244

    • DOI

      10.1016/j.cell.2020.02.010

    • NAID

      120006871293

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Functional Comparison of XPF Missense Mutations Associated to Multiple DNA Repair Disorders2019

    • Author(s)
      Marin M, Ramirez MJ, Carmona MA, Jia N, Ogi T, Bogliolo M, Surralles J.
    • Journal Title

      Genes

      Volume: 10 Issue: 1 Pages: 60-60

    • DOI

      10.3390/genes10010060

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Functional and clinical relevance of novel mutations in a large cohort of patients with Cockayne syndrome.2018

    • Author(s)
      Calmels N, Botta E, Jia N, Fawcett H, Nardo T, Nakazawa Y, Lanzafame M, Moriwaki S, Sugita K, Kubota M, Obringer C, Spitz MA, Stefanini M, Laugel V, Orioli D, Ogi T, Lehmann A.
    • Journal Title

      Journal of Medical Genetics

      Volume: 55 Issue: 5 Pages: 329-343

    • DOI

      10.1136/jmedgenet-2017-104877

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cerebellar ataxia-dominant phenotype in patients with ERCC4 mutations2018

    • Author(s)
      Doi H、Koyano S、Miyatake S、Nakajima S、Nakazawa Y、Kunii M、Tomita-Katsumoto A、Oda K、Yamaguchi Y、Fukai R、Ikeda S、Kato R、Ogata K、Kubota S、Hayashi N、Takahashi K、Tada M、Tanaka K、Nakashima M、Tsurusaki Y et al.
    • Journal Title

      Journal of Human Genetics

      Volume: 63 Issue: 4 Pages: 417-423

    • DOI

      10.1038/s10038-017-0408-5

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] RNA polymerase IIのユビキチン化修飾による転写共役修復開始反応の分子機構とその破綻により発症する哺乳類の神経変性のメカニズム2019

    • Author(s)
      荻朋男
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 転写共役ヌクレオチド除去修復機構に重要なRNAポリメラーゼユビキチン化部位の同定2019

    • Author(s)
      中沢由華, 原雄一郎, 岡泰由, 小峯起, Diana van den Heuvel, 郭朝万, 大学保一, 磯野真由, 何予希, 嶋田繭子, 加藤香奈, 賈楠, 橋下悟, 小谷祐子, 三好由夏, 田中都, 祖父江顕, 光武範吏, 菅波孝祥, 増田章男, 大野欽司, 中田慎一郎, 真下知士, 山中宏二, Martijn S. Luijsterburg, 荻朋男.
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] ChIP-seqを利用したDNA損傷およびヌクレオチド除去修復のモニタリング2019

    • Author(s)
      原雄一郎, 中沢由華, 岡泰由, 小峯起, Diana van den Heuvel, 郭朝万, 大学保一, 磯野真由, 何予希, 嶋田繭子, 加藤香奈, 賈楠, 橋下悟, 小谷祐子, 三好由夏, 田中都, 祖父江顕, 光武範吏, 菅波孝祥, 増田章男, 大野欽司, 中田慎一郎, 真下知士, 山中宏二, Martijn S. Luijsterburg, 荻朋男.
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Alterations in RNA polymerase IIo ubiquitination cause Cockayne syndrome-like premature aging phenotype in mice due to TC-NER defect.2019

    • Author(s)
      Ogi T.
    • Organizer
      International Symposium on XP and other Nucleotide Excision Repair Disorders
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Alterations in RNA polymerase IIo ubiquitination cause Cockayne syndrome-like premature aging phenotype in mice due to TC-NER defect.2019

    • Author(s)
      Nakazawa Y, Ogi T.
    • Organizer
      International Symposium on XP and other Nucleotide Excision Repair Disorders
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ゲノム不安定性疾患群を中心とした希少難治性疾患の次世代マルチオミクス解析と難病プラットフォーム連携による疾患原因変異の検索.2019

    • Author(s)
      荻 朋男.
    • Organizer
      難病プラットフォーム公開シンポジウム
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] DNA損傷依存的なRNAポリメラーゼのユビキチン化修飾の異常はDNA修復経路の欠損マウスで老化表現型を示す.2019

    • Author(s)
      荻 朋男.
    • Organizer
      国立遺伝学研究所・研究集会「ゲノムの維持継承を支える分子基盤の包括的理解とその発展」
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] マルチオミクス解析により同定した重症アイカルディ・ゴーティエ症候群の分子病態解析.2019

    • Author(s)
      岡 泰由, 荻 朋男.
    • Organizer
      第41回日本小児遺伝学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] ゲノム不安定性を示す遺伝性疾患の分子病態.2018

    • Author(s)
      中沢由華, 荻 朋男.
    • Organizer
      第3回名大医薬系3部局交流シンポジウム~岐阜薬科大学・岐阜大学G-CHAIN・ラクオリア創薬合同シンポジウム~
    • Related Report
      2018 Research-status Report
  • [Presentation] RNAポリメラーゼのユビキチン化修飾による転写共役ヌクレオチド除去修復の反応制御とコケイン症候群の病態.2018

    • Author(s)
      荻 朋男.
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Identification of pathogenic mutations in patients with rare diseases using multiomics approaches.2018

    • Author(s)
      Oka Y, Ogi T.
    • Organizer
      Japanese Proteomics Society 2018 Conference (JPrOS 2018), 9th Asia-Oceania Human Proteome Organization (AOHUPO), and 66th Annual Conference on Mass Spectrometry
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Remarks] 名古屋大学生物機能開発利用研究センター 生物産業創出研究室HP

    • URL

      http://bbc.agr.nagoya-u.ac.jp/~graft/index.html

    • Related Report
      2020 Annual Research Report
  • [Remarks] 名古屋大学環境医学研究所HP

    • URL

      http://www.riem.nagoya-u.ac.jp/index.html

    • Related Report
      2019 Research-status Report 2018 Research-status Report
  • [Remarks] 名古屋大学環境医学研究所 発生遺伝分野HP

    • URL

      http://www.riem.nagoya-u.ac.jp/4/genetics/index.html

    • Related Report
      2019 Research-status Report 2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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