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Development of method to evaluate endocrine-disrupting potency of chemicals using secondary sex characteristics of anal fin rays in medaka

Research Project

Project/Area Number 18K11655
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 63030:Chemical substance influence on environment-related
Research InstitutionNagasaki University

Principal Investigator

NAGAE Masaki  長崎大学, 水産・環境科学総合研究科(環境), 教授 (00315227)

Co-Investigator(Kenkyū-buntansha) 高尾 雄二  長崎大学, 水産・環境科学総合研究科(環境), 教授 (20206709)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords環境ホルモン / 内分泌かく乱 / バイオマーカー / 二次性徴 / メダカ / ミナミメダカ / 女性ホルモン / 内分泌かく乱化学物質
Outline of Final Research Achievements

[Objective]: Research objective is to develop new in vivo experimental method to detect endocrine-disrupting potencies, especially in both androgenic and estrogenic potencies of chemicals, using morphological biomarkers of medaka (Oryzias latipes).
[Results]: In this research, “fork formation of anal fin ray”, that is one of the secondary sex characteristics of female medaka, was used morphological biomarker for the detection of estrogenic potency of chemicals. Results of this research suggested that a number and the developing degree of this morphological feature increased by estrogenic stimulation in a dose-dependent manner.
[Conclusion]: By the use female specific estrogenic biomarker “fork formation of anal fin ray” together with male specific secondary sex characteristics, papillary processes on anal fin rays of male medaka, a novel in vivo experimental method to detect both androgenic and estrogenic potencies of chemicals using same experimental animals (medaka) was developed.

Academic Significance and Societal Importance of the Research Achievements

水環境における化学物質汚染の深刻化・多様化により、それが生態系に及ぼすリスクが増大している。化学物質の排水規制や環境中濃度規制に向けて、生物影響の把握が求められている。
本研究では、化学物質の持つ生物影響のうち、内分泌かく乱作用に焦点を絞り、試験生物として有用なメダカの二次性徴形質に着目し、それらを利用した新たな化学物質の性ホルモンかく乱作用を検出するための試験法確立を目指して基礎的知見を集積した。得られた結果は、試験法確立の基盤となり得る有効性を保持しており、新規試験法確立の目処が立った。本研究の成果は、化学物質の生物リスク評価分野に貢献できる点で学術的および社会的意義を備えるものである。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2020 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (8 results)

  • [Journal Article] Field survey of environmental estrogen pollution in the coastal area of Tokyo Bay and Nagasaki City using the Japanese common goby Acanthogobius flavimanus2020

    • Author(s)
      Jing Song, Masaki Nagae, Yuji Takao, Kiyoshi Soyano
    • Journal Title

      Environmental Pollution

      Volume: 258 Pages: 113673-113673

    • DOI

      10.1016/j.envpol.2019.113673

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] Comparison of response sensitivity to steroid hormones between two species of medaka, Minami-medaka (Oryzias latipes) and Kitano-medaka (Oryzias sakaizumii)2020

    • Author(s)
      Masaki Nagae, Daisuke Iino, Madoka Moriwaki, Kanako Yamashiro, Yuji Takao, Norihisa Tatarazako and Taisen Iguchi
    • Organizer
      The 22th UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals
    • Related Report
      2020 Annual Research Report
  • [Presentation] Continuous field survey of environmental estrogens (EEs) pollution and study on combined effects of EEs and pharmaceuticals on fish reproduction2020

    • Author(s)
      Kiyoshi Soyano and Masaki Nagae
    • Organizer
      The 22th UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals
    • Related Report
      2020 Annual Research Report
  • [Presentation] Comparison of response sensitivity to steroid hormones between two species of medaka, Minami-medaka (Oryzias latipes) and Kitano-medaka (Oryzias sakaizumii)2019

    • Author(s)
      Masaki Nagae, Daisuke Iino, Madoka Moriwaki, Kanako Yamashiro, Yuji Takao, Norihisa Tatarazako and Taisen Iguchi
    • Organizer
      The 21th UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals.
    • Related Report
      2019 Research-status Report
  • [Presentation] Influence of environmental estrogens on fish reproduction in Japanese coastal waters2019

    • Author(s)
      Kiyoshi Soyano, Masaki Nagae
    • Organizer
      The 21th UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals
    • Related Report
      2019 Research-status Report
  • [Presentation] 下水処理後の排水に含まれる粒子状物質による性ホルモン遮断効果2019

    • Author(s)
      長江真樹、柴田南、征矢野清、高尾雄二
    • Organizer
      第28回環境化学討論会
    • Related Report
      2019 Research-status Report
  • [Presentation] 魚類の外因性性ホルモン取り込みに及ぼす下水処理排水中粒子状物質の遮断効果2019

    • Author(s)
      長江真樹、柴田南、西哲平、征矢野清、高尾雄二
    • Organizer
      第22回環境ホルモン学会研究発表会
    • Related Report
      2019 Research-status Report
  • [Presentation] メダカ臀鰭二次性徴形質のEDCsバイオマーカーへの利用2018

    • Author(s)
      加島未奈子、飯野大輔、今西颯、柴田南、高尾雄二、長江真樹
    • Organizer
      環境ホルモン学会・第21回研究発表会
    • Related Report
      2018 Research-status Report
  • [Presentation] Application of morphological sexual differenciation on anal fin rays in medaka (Oryzias latipes) for EDCs biomarker2018

    • Author(s)
      Minako Kajima, Daisuke Iino, Tatsuru Imanishi, Minami Shibata, Masaki Nagae
    • Organizer
      The 15th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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