Visualization of cellular response to cyclic pressure stress

• Project/Area Number: 18K12033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Okayama University
• Principal Investigator: Morimatsu Masatoshi 岡山大学, 医歯薬学域, 助教 (70580934)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 静水圧 / 周期的圧力 / メカノバイオロジー / 機械刺激 / 圧力 / 咬合圧

Outline of Final Research Achievements

Activities of daily living such as walking and sitting pressurize articular cartilage. However, the effect of pressurization on chondrocytes is poorly understood due to the lack of methods that directly observe cells under high pressure conditions. Here we developed high hydrostatic system to observe human cell dynamics under high pressure condition. We also found high hydrostatic pressure regulates signaling pathway in chondrocyte. Our results suggest the model of the homeostasis of articular cartilage under high pressure conditions.

Academic Significance and Societal Importance of the Research Achievements

高齢化社会における生活の質(QOL)の維持や向上は必須であるが、歩行や咀嚼等の生命活動に伴ったずり応力、圧力、伸展圧縮等の様々な機械刺激が生体組織に必ず生じるため、機械刺激を考慮した細胞や器官の受容応答メカニズムの解明の遂行なくして、QOLの向上に向けた根本的な改善に繋がらない。本研究では、細胞の圧力受容応答メカニズムに迫る研究であった。また、本研究成果は、軟骨組織のリモデリング機構の解明に繋がる知見であり、今後の治療応用にの発展に寄与するものと考える。

Research Products

• [Journal Article] Increased hydrostatic pressure induces nuclear translocation of DAF-16/FOXO in C.elegans (2020)
  • Author(s): Watanabe Naoshi、Morimatsu Masatoshi、Fujita Ayano、Teranishi Mika、Sudevan Surabhi、Watanabe Masaru、Iwasa Hiroaki、Hata Yutaka、Kagi Hiroyuki、Nishiyama Masayoshi、Naruse Keiji、Higashitani Atsushi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 523 Issue: 4 Pages: 853-858
  • DOI: 10.1016/j.bbrc.2020.01.047
  • Peer Reviewed / Open Access
• [Presentation] SMAD 3 Signaling Pathway in Chondrocytes under High Hydrostatic Pressure (2021)
  • Author(s): Xinxuan Li, Keiji Naruse, Masatoshi Morimatsu
  • Organizer: Materials, Mimics, and Microfluidics: Engineering Tools for Mechanobiology (MBI 3M) satellite poster session in Japan
• [Presentation] 高圧刺激下での軟骨細胞の動態計測 (2021)
  • Author(s): 森松 賢順，Li Xinxuan，西山 雅祥, 成瀬 恵治
  • Organizer: 第44回日本生体医工学会中国四国支部大会
• [Presentation] High hydrostatic pressure regulate TGF-β signaling pathway in human chondrocytes (2021)
  • Author(s): M. Morimatsu, X. Li, M.Nishiyama, K.Naruse
  • Organizer: 第59回日本生物物理学会年会
• [Presentation] 高圧刺激下での細胞機能計測 (2021)
  • Author(s): 森松賢順
  • Organizer: メカノバイオ討論会2021
• [Presentation] HIGH HYDROSTATIC PRESSURE INDUCES NUCLEAR TRANSLOCATION OF FOXO3 (2021)
  • Author(s): Masatoshi Morimatsu, Ayano Fujita, Masayoshi Nishiyama, Keiji Naruse
  • Organizer: Biophysical Society Annual Meeting 2021
  • Int'l Joint Research
• [Presentation] 高圧下での細胞動態イメージング (2020)
  • Author(s): 森松賢順, 藤田彩乃, 寺町一希, 成瀬恵治
  • Organizer: 第59回 日本生体医工学会大会
  • Invited
• [Presentation] Cell dynamics under high hydrostatic pressure conditions (2020)
  • Author(s): Masatoshi Morimatsu, Masayoshi Nishiyama, Keiji Naruse
  • Organizer: 第58回日本生物物理学会年会
  • Invited
• [Presentation] CHONDROCYTE DYNAMICS UNDER HIGH HYDROSTATIC PRESSURE (2020)
  • Author(s): Masatoshi Morimatsu, Kazuki Teramachi, Masayoshi Nishiyama, Keiji Naruse
  • Organizer: Biophysical Society Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] 生理的圧力下での細胞動態計測 (2019)
  • Author(s): 森松賢順, 藤田彩乃, 綾晃記, 寺町一希, 稲葉晃帆, 西山雅祥, 成瀬恵治
  • Organizer: 第58回 日本生体医工学会大会
  • Invited
• [Presentation] Direct observation of cell mechanics under high hydrostatic pressure (2019)
  • Author(s): Masatoshi Morimatsu, Keiji Naruse
  • Organizer: 第57回日本生物物理学会年会
  • Invited
• [Presentation] Direct observation of cell mechanics under the physiological high hydrostatic pressure condition (2019)
  • Author(s): Masatoshi Morimatsu, Masayoshi Nishiyama, Keiji Naruse
  • Organizer: 第42回日本分子生物学会年会
  • Invited
• [Presentation] 高圧下での細胞動態イメージング (2018)
  • Author(s): 森松 賢順, 藤田 彩乃, 綾 晃記, 西山 雅祥, 成瀬 恵治
  • Organizer: 第57回日本生体医工学会大会
• [Presentation] Mechanical Regulation of Cellular Homeostasis (2018)
  • Author(s): Masatoshi Morimatsu
  • Organizer: iCeMS Mini-Symposium on Mechanobiology
  • Int'l Joint Research / Invited
• [Presentation] 生理的高圧下でのリアルタイム細胞動態計測 (2018)
  • Author(s): 森松賢順, 藤田彩乃, 綾晃記, 寺町一希, 稲葉晃帆, 成瀬恵治, 西山 雅祥
  • Organizer: 第59回高圧討論会
• [Presentation] Direct observation of chondrocytes under high hydrostatic pressure (2018)
  • Author(s): Masatoshi Morimatsu, Ayano Fujita, Masayoshi Nishiyama, Keiji Naruse
  • Organizer: 2018 ASCB | EMBO Annual Meeting
  • Int'l Joint Research