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Development of artificial laminin gel platform for acinar structure regeneration

Research Project

Project/Area Number 18K12085
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 90120:Biomaterials-related
Research InstitutionKitasato University

Principal Investigator

Hozumi Kentaro  北里大学, 北里大学保健衛生専門学院, 講師 (10453804)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsラミニン / 基底膜 / キトサン / ハイドロゲル / 活性ペプチド / 細胞外マトリックス / ペプチド / 細胞培養プラットフォーム / 細胞分化 / 血管新生阻害 / 細胞接着 / 唾液腺細胞 / インテグリン / クロストーク / 線維芽細胞 / 組織再生 / ゲル
Outline of Final Research Achievements

The major component of Matrigel is laminin, a multi-functional extracellular matrix protein. Laminin is one of the important extracellular matrix proteins which promotes salivary gland cell differentiation. In this study, we constructed artificial laminin using the biologically active peptide and polysaccharide hydrogel as a cell culture platform. We could partially mimic the function of laminin. When we culture the salivary gland cells on Matrigel and artificial laminin, the shape of small glands (acinar form) was similar. But the function of the gland were not equal. We identified that turnover of exogenous laminin and secreted laminin is important to promote the functional gland.

Academic Significance and Societal Importance of the Research Achievements

再生医療に利用可能な人工細胞外マトリックスとしてペプチドー高分子多糖ハイドロゲルを作製し分化誘導型培養プラットフォームの開発をおこなった。誘導された腺器官の機能は生体由来の細胞外マトリックスを用いた場合より劣っていた。この理由として外因性の細胞外マトリックスと内因性の細胞外マトリックスのターンオーバが重要であることが示唆された。内因性の細胞外マトリックスをつなぎ止め、かつ細胞によって認識・分解をされるデザインを培養プラットフォームに組み込むことが、培養プラットフォームの設計にあたっての一つのキーとなる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (25 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 6 results) Presentation (14 results) (of which Int'l Joint Research: 9 results,  Invited: 1 results) Remarks (2 results)

  • [Int'l Joint Research] National Institutes of Health(米国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] 米国NIH(米国)

    • Related Report
      2019 Research-status Report
  • [Journal Article] 人工ラミニンの構築を目指した細胞培養プラットフォームの開発2021

    • Author(s)
      保住建太郎、野水基義
    • Journal Title

      BioClinica

      Volume: 36 Pages: 68-73

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Fibulin-7 C-terminal fragment and its active synthetic peptide suppress choroidal and retinal neovascularization2020

    • Author(s)
      Ikeuchi Tomoko、Kanan Yogita、Long Da、de Vega Susana、Hozumi Kentaro、Nomizu Motoyoshi、Campochiaro Peter A.、Yamada Yoshihiko
    • Journal Title

      Microvascular Research

      Volume: 129 Pages: 103986-103986

    • DOI

      10.1016/j.mvr.2020.103986

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Characterization of dystroglycan binding in adhesion of human induced pluripotent stem cells to laminin-511 E8 fragment2019

    • Author(s)
      Sugawara Yumika、Hamada Keisuke、Yamada Yuji、Kumai Jun、Kanagawa Motoi、Kobayashi Kazuhiro、Toda Tatsushi、Negishi Yoichi、Katagiri Fumihiko、Hozumi Kentaro、Nomizu Motoyoshi、Kikkawa Yamato
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 1-12

    • DOI

      10.1038/s41598-019-49669-x

    • NAID

      120006728095

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of active sequences in human laminin α5 G domain2019

    • Author(s)
      Kumai Jun、Yamada Yuji、Hamada Keisuke、Katagiri Fumihiko、Hozumi Kentaro、Kikkawa Yamato、Nomizu Motoyoshi
    • Journal Title

      Journal of Peptide Science

      Volume: 25 Issue: 12 Pages: 1-9

    • DOI

      10.1002/psc.3218

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of specific integrin cross-talk for dermal fibroblast cell adhesion using a mixed peptide-chitosan matrix2019

    • Author(s)
      Hozumi Kentaro、Teranishi Yui、Enomoto Sayaka、Katagiri Fumihiko、Kikkawa Yamato、Nomizu Motoyoshi
    • Journal Title

      Journal of Biomaterials Applications

      Volume: 33 Issue: 7 Pages: 893-902

    • DOI

      10.1177/0885328218823457

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cell adhesion activity of peptides conjugated to polysaccharides2018

    • Author(s)
      Hozumi K, Nomizu M
    • Journal Title

      Curr Protoc Cell Biol.

      Volume: 80(1) Issue: 1

    • DOI

      10.1002/cpcb.53

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mixed Peptide-Conjugated Chitosan Matrices as Multi-Receptor Targeted Cell-Adhesive Scaffolds2018

    • Author(s)
      Hozumi K, Nomizu M
    • Journal Title

      Int J Mol Sci.

      Volume: 19(9) Issue: 9 Pages: 2713-2713

    • DOI

      10.3390/ijms19092713

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Development of cell culture platforms using dicarboxylic acids crosslinked chitosan hydrogels2020

    • Author(s)
      Kentaro Hozumi, Chie Takahashi, Keisuke Hamada, Yuji Yamada, Yamato Kikkawa, Motoyoshi Nomizu
    • Organizer
      第52回日本結合組織学会
    • Related Report
      2020 Research-status Report
  • [Presentation] ラミニン由来活性ペプチドを用いた細胞接着性マトリックスの開発2020

    • Author(s)
      保住建太郎
    • Organizer
      日本接着学会東北支部講演会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 小胞体ストレス誘発性脂肪肝におけるビタミン E 同族体摂取の影響2020

    • Author(s)
      髙橋知衣、保住建太郎、荒川真大、五十嵐千尋、今坂萌、髙橋慧、長谷川知輝、田中理恵子、清瀬千佳子
    • Organizer
      第 31 回ビタミンE研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] 細胞接着ペプチドを固定化したキトサン/ジカルボン酸ハイドロゲルの開発2019

    • Author(s)
      保住建太郎、高橋知衣、山田隼人、内山千尋、小林彩乃、平賀瑠衣、林良雄、野水基義
    • Organizer
      第56回ペプチド討論会
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Cell Adhesive Peptide Conjugated Chitosan Hydrogel as Salivary Gland Cell Culture Scaffolds2019

    • Author(s)
      K. Hozumi, C. Takahashi, H. Yamada, C. Uchiyama, A. Kobayashi, R. Hiraga, M. Nomizu
    • Organizer
      ASCB/EMBO 2019 meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Identification of Biologically Active Site in Mouse Laminin Alpha3b Chain N Terminal Region2019

    • Author(s)
      F. Katagiri, M. Hashidume, N. Midorikawa, S. Hiraoka, K. Kimura, R. Takayanagi, K. Hozumi, Y. Kikkawa, M. Nomizu, Y. Yamada
    • Organizer
      ASCB/EMBO 2019 meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characterization of Dystroglycan Binding in Adhesion of Human Induced Pluripotent Stem Cells to Laminin‐511 E8 Fragment2019

    • Author(s)
      Y. Kikkawa, Y. Sugawara, K. Hamada, Y. Yamada, J. Kumai, M. Kanagawa, K. Kobayashi, T. Toda, Y. Negishi, F. Katagiri, K. Hozumi, M. Nomizu
    • Organizer
      ASCB/EMBO 2019 meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 二種混合ペプチド-多糖マトリックスを用いた インテグリンサブタイプ間クロストークの評価とメカニズム解明2018

    • Author(s)
      保住建太郎、寺西優衣、片桐文彦、吉川大和、野水基義
    • Organizer
      第67回高分子討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] Internalization of CD239, a laminin a5 receptor, in human breast cancer: a novel antigen for antibody-drug conjugates2018

    • Author(s)
      Y. Kikkawa, Y. Enomoto-Okawa, A. Fujiyama, T. Fukuhara, N. Harashima, Y. Sugawara, K. Ikari, Y Negishi, F. Ktagiri, K. Hozumi, M. Nomizu, Y. Ito
    • Organizer
      American Society of Matrix Biology 2018 Biennial Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Modification of chain-specific cell adhesion sequences in the short arm region of laminin b chain2018

    • Author(s)
      K. Hamada, Y. Gu, J. Kumai, K. Nakamura,F. Katagiri, K. Hozumi, Y. Kikkawa, M. Nomizu
    • Organizer
      10th International Peptide Symposium 第55回ペプチド討論会
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Identification of a-dystroglycan binding sequences in the laminin a2 chain LG4-5 modules using the peptide-chitosan matrix2018

    • Author(s)
      G. Zhang, K. Hamada, J. Kumai, F. Katagiri, K. Hozumi, Y. Kikkawa, M. Nomizu
    • Organizer
      10th International Peptide Symposium 第55回ペプチド討論会
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Attachment of human iPS cells to dystroglycan-binding peptides derived from laminin-511 E8 fragment2018

    • Author(s)
      Y. Kikkawa, Y. Sugawara, K. Hamada, J. Kumai, M. Kanagawa, K. Kobayashi, T. Toda, F. Katagiri, K. Hozumi, M. Nomizu
    • Organizer
      American Society of Cell Biology/Europian Molecular Biology Organization 2018 meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Identification of alpha-dystroglycan bindingpeptide sequences in the laminin alpha2 chain LG4-5 modules using the peptides-chitosan matrix2018

    • Author(s)
      K. Hamada, G. Zhang, J. Kumai, F. Ktagiri, K. Hozumi, Y. Kikkawa, M. Nomizu
    • Organizer
      American Society of Cell Biology/Europian Molecular Biology Organization 2018 meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 乳癌細胞において内在化するラミニンα5鎖受容体 CD239に対する抗体-薬物結合体の作製2018

    • Author(s)
      吉川大和、大川(榎元)友里恵、藤山愛子、福原武志、原島望、菅原由美香、碇和樹、根岸洋一、片桐文彦、保住建太郎、野水基義、伊東祐二
    • Organizer
      第50回日本結合組織学会学術大会
    • Related Report
      2018 Research-status Report
  • [Remarks] リサーチマップ

    • URL

      https://researchmap.jp/KentaroHozumi

    • Related Report
      2020 Research-status Report
  • [Remarks] 北里大学保健衛生専門学院管理栄養科

    • URL

      http://kerid-web.kitasato-u.ac.jp/Profiles/108/0010783/profile.html

    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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