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Development of a new clustering technique for biological component analyses

Research Project

Project/Area Number 18K12112
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 90130:Medical systems-related
Research InstitutionJuntendo University (2022-2023)
International University of Health and Welfare (2018-2021)

Principal Investigator

Sato Shouichi  順天堂大学, 医療科学部, 教授 (90803255)

Co-Investigator(Kenkyū-buntansha) 市原 清志  山口大学, 大学院医学系研究科, 学術研究員(寄附金) (10144495)
山下 哲平  東海大学, 医学部, 助教 (50617420)
Project Period (FY) 2018-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsクロマトグラフィー / フローサイトメトリー法 / クラスター解析 / 正規分布 / シミュレーションプログラム / 分離分析 / AI・ビッグデータ時代 / Box-Cox変換 / パラメトリック処理 / 基準範囲設定 / 画像解析 / ノイズ / 方法間比較 / 画像分類 / フィッティング関数 / スキャッタグラム / 高圧液体クロマトグラム / 重なりのあるクロマトグラム / クラスター分析 / 自己分配方式 / 画像処理方式 / 反復切断補正法 / フローサイトメトリー
Outline of Final Research Achievements

A new cluster analysis method has been developed to solve the problem of separating data in which multiple components overlap in separation analysis technology. This technology is particularly effective for overcoming problems such as overlapping between cell components, the appearance of abnormal cells, and the introduction of noise in the classification of white blood cells. Specifically, by using image processing to accurately estimate the types and distribution of cells, and by mathematically separating the overlapping of multiple components using multiple normal distribution theory and iterative truncation correction, it is possible to obtain clear analytical results. We have further developed this technology and improved it into an algorithm that can be applied to the separation of various mixed distributions, and have reported on it at various academic conferences.

Academic Significance and Societal Importance of the Research Achievements

本研究では、分離分析技術で問題となる成分の重なりを解決する手法として、画像処理、自己分配方式、反復切断補正法を組み合わせた新しいクラスター分析法を開発した。特に、白血球分類において、細胞成分の正確な分離とノイズ除去を行うことが可能になり、複雑な臨床データに対応する分析精度が向上することを確認した。この技術は、臨床検査の精度向上に寄与し、様々な分離分析場面に対応できるため、医療現場での診断の信頼性を高める社会的意義があると考えられる。

Report

(7 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (27 results)

All 2024 2023 2022 2021 2020

All Journal Article (17 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 13 results,  Open Access: 5 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 7 results) Book (1 results)

  • [Journal Article] AI・ビッグデータ時代の臨床検査のための情報科学・5 -検査のためのAI入門-深層学習による画像解析2023

    • Author(s)
      佐藤 正一
    • Journal Title

      臨床検査

      Volume: 67 Issue: 12 Pages: 1392-1397

    • DOI

      10.11477/mf.1542203482

    • ISSN
      0485-1420, 1882-1367
    • Year and Date
      2023-12-15
    • Related Report
      2023 Annual Research Report
  • [Journal Article] Forecasting supply and demand for medical technologists from open data2023

    • Author(s)
      古賀 秀信、丸田 秀夫、深澤 恵治、益田 泰蔵、佐藤 正一、根本 誠一、白石 元気
    • Journal Title

      Japanese Journal of Medical Technology

      Volume: 72 Issue: 4 Pages: 522-531

    • DOI

      10.14932/jamt.23-48

    • ISSN
      0915-8669, 2188-5346
    • Year and Date
      2023-10-25
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] AI・ビッグデータ時代の臨床検査のための情報科学・2 検査の精度管理に必要な統計学2023

    • Author(s)
      佐藤 正一
    • Journal Title

      臨床検査

      Volume: 67 Issue: 6 Pages: 668-676

    • DOI

      10.11477/mf.1542203329

    • ISSN
      0485-1420, 1882-1367
    • Year and Date
      2023-06-15
    • Related Report
      2023 Annual Research Report
  • [Journal Article] Relationship between a deep learning model and liquid‐based cytological processing techniques2023

    • Author(s)
      Ikeda Katsuhide、Sakabe Nanako、Maruyama Sayumi、Ito Chihiro、Shimoyama Yuka、Oboshi Wataru、Komene Tetsuya、Yamaguchi Yoshitaka、Sato Shouichi、Nagata Kohzo
    • Journal Title

      Cytopathology

      Volume: - Issue: 4 Pages: 308-317

    • DOI

      10.1111/cyt.13235

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Specimen Processing Technique on Cell Detection and Classification by Artificial Intelligence2023

    • Author(s)
      Maruyama Sayumi、Sakabe Nanako、Ito Chihiro、Shimoyama Yuka、Sato Shouichi、Ikeda Katsuhide
    • Journal Title

      American Journal of Clinical Pathology

      Volume: - Issue: 5 Pages: 448-454

    • DOI

      10.1093/ajcp/aqac178

    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of liquid‐based cytology fixing solution on immunocytochemistry: Efficacy of antigen retrieval in cytologic specimens2023

    • Author(s)
      Sakabe Nanako、Maruyama Sayumi、Ito Chihiro、Shimoyama Yuka、Sudo Kazuhisa、Sato Shouichi、Ikeda Katsuhide
    • Journal Title

      Diagnostic Cytopathology

      Volume: 51 Issue: 9 Pages: 546-553

    • DOI

      10.1002/dc.25178

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Critical appraisal of two Box-Cox formulae for their utility in determining reference intervals by realistic simulation and extensive real-world data analyses2023

    • Author(s)
      Ichihara Kiyoshi、Yamashita Teppei、Kataoka Hiromi、Sato Shoichi
    • Journal Title

      Computer Methods and Programs in Biomedicine

      Volume: 242 Pages: 107820-107820

    • DOI

      10.1016/j.cmpb.2023.107820

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 僧帽弁形成術前後における左室内腔渦流評価2023

    • Author(s)
      伊藤 記彦 , 諏訪部 章 , 田代 敦 , 山口 良考 , 片山 博徳 , 佐藤 正一
    • Journal Title

      医学と生物学

      Volume: 163 Pages: 1-13

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between Liquid-Based Cytology Preservative Solutions and Artificial Intelligence: Liquid-Based Cytology Specimen Cell Detection Using YOLOv5 Deep Convolutional Neural Network2022

    • Author(s)
      Ikeda Katsuhide、Sakabe Nanako、Maruyama Sayumi、Ito Chihiro、Shimoyama Yuka、Sato Shouichi、Nagata Kohzo
    • Journal Title

      Acta Cytologica

      Volume: 66 Issue: 6 Pages: 542-550

    • DOI

      10.1159/000526098

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of large canine hepatocyte spheroids by mixing vascular endothelial cells and canine adipose-derived mesenchymal stem cells2022

    • Author(s)
      Ichikawa Akito、Neo Sakurako、Nukui Ryouhei、Yamada Yoko、Nitta Suguru、Iwaki Hidetoshi、Yanagi Yusuke、Nakayama Koichi、Sato Shoichi、Tateishi Satoko、Hisasue Masaharu
    • Journal Title

      Regenerative Therapy

      Volume: 19 Pages: 1-8

    • DOI

      10.1016/j.reth.2021.11.007

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Health Effects of Drinking Water Produced from Deep Sea Water: A Randomized Double-Blind Controlled Trial2022

    • Author(s)
      Takeuchi Hiroaki、Yoshikane Yu、Takenaka Hirotsugu、Kimura Asako、Islam Jahirul Md.、Matsuda Reimi、Okamoto Aoi、Hashimoto Yusuke、Yano Rie、Yamaguchi Koichi、Sato Shouichi、Ishizuka Satoshi
    • Journal Title

      Nutrients

      Volume: 14 Issue: 3 Pages: 581-581

    • DOI

      10.3390/nu14030581

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 次元圧縮とクラスター解析による可視化と臨床応用2022

    • Author(s)
      佐藤正一
    • Journal Title

      臨床化学

      Volume: 51 Pages: 12-20

    • Related Report
      2021 Research-status Report
  • [Journal Article] 臨床検査におけるAIの概略-AIが臨床検査でできること2021

    • Author(s)
      佐藤正一
    • Journal Title

      日本臨床検査医学会誌

      Volume: 69 Pages: 44-348

    • Related Report
      2021 Research-status Report
  • [Journal Article] 今月の特集2 EBLM(evidence based laboratory medicine)の新展開 クラスター分析の臨床検査への応用2020

    • Author(s)
      佐藤正一
    • Journal Title

      臨床検査

      Volume: 64 Issue: 5 Pages: 600-608

    • DOI

      10.11477/mf.1542202370

    • ISSN
      0485-1420, 1882-1367
    • Year and Date
      2020-05-15
    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] 特集 教えて! 健診/検診"ホントのところ"-エビデンスを知り,何を伝えるか 健診/検診の理解を深める なぜ正常範囲ではなく"基準範囲"なのか-"異常"は本当に異常なのか?2020

    • Author(s)
      佐藤正一,市原清志
    • Journal Title

      medicina

      Volume: 57 Issue: 6 Pages: 827-831

    • DOI

      10.11477/mf.1402226977

    • ISSN
      0025-7699, 1882-1189
    • Year and Date
      2020-05-10
    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Differences in cytokine expression after a stimulation with Escherichia coli and Porphyromonas gingivalis or lipopolysaccharides from these bacteria2020

    • Author(s)
      Nobuyasu Y, Yuna K, Misaki O, Wataru O, Shoichi S, Mirai Y, Takehiro N
    • Journal Title

      Journal of Life Sciences Research

      Volume: 7(1) Pages: 13-20

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Drinking Refined Deep-Sea Water Improves the Gut Ecosystem with Beneficial E ects on Intestinal Health in Humans: A Randomized Double-Blind Controlled Trial2020

    • Author(s)
      Hiroaki Takeuchi 1,2,*, Keiro Higuchi 3, Yu Yoshikane 4, Ryo Takagi 2, Shinji Tokuhiro 2,Koichi Takenaka 5, Wataru Oboshi 1, Asako Kimura 1, Jahirul Md. Islam 1, Ayami Kaneko 1,Shouichi Sato 1 and Satoshi Ishizuka
    • Journal Title

      Nutrients

      Volume: 12 Issue: 9 Pages: 2646-2646

    • DOI

      10.3390/nu12092646

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] The current state of Artificial Intelligence (AI ) in clinical laboratories towards its practice.2023

    • Author(s)
      Sato Shoichi
    • Organizer
      14th Asia-Pacific Forum of Medical Laboratory Science
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 不確かさの理論解説2023

    • Author(s)
      佐藤正一
    • Organizer
      日本医学検査学会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 臨床検査領域におけるAIの基礎から臨床応用と課題2023

    • Author(s)
      佐藤正一
    • Organizer
      日本臨床検査医学会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 方法間比較のための回帰直線・偏差図の問題点と使い分け2023

    • Author(s)
      佐藤正一
    • Organizer
      日本臨床検査医学会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] The current state of Artificial Intelligence (AI ) in clinical laboratories towards its practice2023

    • Author(s)
      佐藤正一
    • Organizer
      The Application of Artificial Intelligence in Laboratory Medicine
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 方法間比較のための回帰直線・偏差図の問題点と使い分け2022

    • Author(s)
      佐藤正一
    • Organizer
      日本臨床検査医学会学術集会
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] クラスター解析と次元圧縮事例2021

    • Author(s)
      佐藤正一
    • Organizer
      第67回日本臨床検査医学会学術集会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] HPLCにおける混合分布に対する正確な成分定量法の開発2020

    • Author(s)
      佐藤正一
    • Organizer
      第67回日本臨床検査医学会学術集会
    • Related Report
      2020 Research-status Report
  • [Presentation] メタロバランス法を用いたクラスター分析から見るがんリスクスクリーニング検査法の有用性2020

    • Author(s)
      佐藤正一
    • Organizer
      第60回日本臨床化学会年次学術集会
    • Related Report
      2020 Research-status Report
  • [Book] 臨床検査総合管理学 第3版2024

    • Author(s)
      高木康・三村邦裕 編著/五十嵐清子・海渡健・片岡浩巳・佐藤正一・諏訪部章・清宮正徳・中原貴子・三浦ひとみ・脇田満 執筆
    • Total Pages
      23
    • Publisher
      医歯薬出版
    • ISBN
      9784263223994
    • Related Report
      2023 Annual Research Report

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Published: 2018-04-23   Modified: 2025-01-30  

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