| Project/Area Number |
18K13028
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 08030:Family and consumer sciences, and culture and living-related
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| Research Institution | Nakamura Gakuen University Junior College |
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Principal Investigator |
Nagamitsu Hiroshi 中村学園大学短期大学部, 食物栄養学科, 准教授 (20333271)
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| Project Period (FY) |
2018-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 変形性関節症 / 軟骨 / MMP-13 / ADAMTS-5 |
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| Outline of Final Research Achievements |
Osteoarthritis (OA) is a disease characterized by degeneration and loss of articular cartilage. In particular, OA in the knee joint affects a large number of elderly people, and the development of treatment methods is indispensable to decrease in quality of life and an increase in medical costs. MMP-13, a proteolytic enzyme involved in joint destruction, and ADAMTS-5, an aggrecan degrading enzyme, are important therapeutic targets. In this study, luciferase activity was used as a tool to efficiently search for food ingredients that regulate the expression of these proteins.
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| Academic Significance and Societal Importance of the Research Achievements |
ルシフェラーゼ活性を指標としたことで、多検体でも迅速に測定することが可能になることが期待される。また、実験動物でのOA進行度の評価として、関節痛の疼痛閾値測定と、左右の体重バランス変化は、解剖することなく経時的にかつ安価に測定できるメリットがある。今回測定した食品成分は限られたが、ルシフェラーゼ活性で効果の見込まれた成分が発見されれば、動物での試験と併せて効率的に評価できると考えられる。
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