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Identification of enzymes that degrades nylon 4 and the biodegradation behaviors of nylon 4

Research Project

Project/Area Number 18K14265
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 34030:Green sustainable chemistry and environmental chemistry-related
Research InstitutionTokai University (2021)
Prefectural University of Kumamoto (2018-2020)

Principal Investigator

Hokamura Ayaka  東海大学, 農学部, 講師 (50762704)

Project Period (FY) 2019-02-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsナイロン4 / ポリアミド4 / 生分解性プラスチック / 分解酵素
Outline of Final Research Achievements

In this study, Pseudoxanthomonas strains were isolated for the first time as nylon 4 -degrading bacteria. GABA monomers and oligomers were detected by LC/MS analysis of supernatant after cultivation of isolated nylon 4-degrading strains using medium with nylon 4 film. Furthermore, it was suggested that nylon 4 was degraded from the surface by the SEM analysis. As regards the identification of enzyme degrades nylon 4, a part of genes that is expected to be the enzyme were amplified by PCR reaction.

Academic Significance and Societal Importance of the Research Achievements

プラスチックは、軽くて丈夫であることや安価で易加工性であることから、我々の生活に欠かせない素材となっているが、その構造の安定性ゆえに使用後の廃棄方法や環境への影響が大きな問題となっている。ナイロン4を分解する新たな菌を発見し、その分解性の評価や酵素同定の一部を行った本研究は、分解制御可能な生分解性ナイロン4の実用化に向けて社会的意義のある研究といえる。単離した分解菌の酵素同定とその性質を決めることで、環境調和型で生産性の高いシステムが構築でき、今後ますます深刻化しうる環境問題の解決に貢献できると予測される。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (4 results)

All 2022 2021 2020

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results)

  • [Journal Article] Purification and characterization of an enzyme that degrades polyamide 4 into gamma-aminobutyric acid oligomers from Pseudoxanthomonas sp. TN-N12022

    • Author(s)
      Yurika Sasanami, Masayoshi Honda, Haruka Nishiki, Koichiro Tachibana, Hideki Abe, Ayaka Hokamura, Miwa Yamada
    • Journal Title

      Polymer Degradation and Stability

      Volume: 197 Pages: 109868-109868

    • DOI

      10.1016/j.polymdegradstab.2022.109868

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] Pseudoxanthomonas sp. TN-N1由来生分解性プラスチックナイロン 4 分解酵素の諸性質解明2022

    • Author(s)
      佐々浪 由梨香、本田 正義、外村 彩夏、阿部 英喜、山田 美和
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 生分解性プラスチックナイロン4分解酵素の精製と諸性質解明2021

    • Author(s)
      佐々浪 由梨香、外村 彩夏、河合 盛進、鈴木 宏昭、山田 美和
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ナイロン4分解菌の単離とその分解性評価2020

    • Author(s)
      外村彩夏、森上佳衣子、橘弘一郎、山田美和、本田正義、阿部英喜、下田誠也
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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