| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The aim of this study was modifying ribosome, which is the organelle producing proteins in a cell, to improve the incorporation efficiency of non-proteinogenic amino acids into peptide. We successfully developed a method to pick up the desired mutant ribosome from the pool of highly diverse mutant ribosomes containing random mutations in their rRNA sequences. Although we found the rRNA sequence with an improved activity to introduce D-amino acid into peptide, the expression and purification of the mutant ribosome from E. coli cells were difficult because the structural stability of the mutant ribosome was low.
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