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Creation of the plant cultured cells to produce the functional products

Research Project

Project/Area Number 18K14389
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38030:Applied biochemistry-related
Research InstitutionToyama Prefectural University

Principal Investigator

Kitaoka Naoki  富山県立大学, 工学部, 助教 (20785547)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords植物培養細胞 / 遺伝子組換え / タケ / 二次代謝産物 / 生合成 / 有用物質生産 / 植物二次代謝産物
Outline of Final Research Achievements

We generated the transformed bamboo cells which efficiently produced the functional products. Firstly, we generated the bamboo cells which were transformed by hydroxycinnamic acid hydratase/aldolase (PpHCHL) from Pseudomonas putida. In the PpHCHL-transformed cells, the high accumulation of glucose conjugates of vanillic acid and 4-hydroxybenozic acid were observed. Secondary, we generated the bamboo cells which were transformed to express phenolic acid decarboxylase (BaPAD) from Bacillus amyloliquefacience. In the BaPAD transformed cells, primeverosides of 4-vinylpheol and 4-vinylguaiacol were produced.

Academic Significance and Societal Importance of the Research Achievements

タケ培養細胞を宿主としてヒドロキシ安息香酸およびビニルフェノールの配糖体を生産可能であることを示した。特に、ヒドロキシ安息香酸のグルコース配糖体の生産量は培地1 Lあたり1.7 gに達し、タケ培養細胞が極めて優れた物質生産宿主であることを示すことができた。
また、ヒドロキシ安息香酸とビニルフェノールで異なる糖が結合した配糖体が得られたことから、タケ培養細胞内における配糖体の生成機構に興味が持たれる。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2020 2019

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Bioproduction of glucose conjugates of 4-hydroxybenzoic and vanillic acids using bamboo cells transformed to express bacterial 4-hydroxycinnamoyl-CoA hydratase/lyase2020

    • Author(s)
      Naoki Kitaoka, Taiji Nomura, Shinjiro Ogita, Yasuo Kato
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: in press Issue: 1 Pages: 89-97

    • DOI

      10.1016/j.jbiosc.2020.02.010

    • NAID

      40022293631

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Bioproduction of glucose esters of vanillic acid and 4-hydroxybenzoic acid in the transformed bamboo cells expressing bacterial 4-hydroxycinnamoyl-CoA hydratase/lyase2020

    • Author(s)
      Naoki Kitaoka, Taiji Nomura, Shinjiro Ogita, Yasuo Kato
    • Organizer
      Gordon Research Conference Plant Metabolic Engineering
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bacillus amyloliquefaciens由来phenolic acid decarboxylaseを発現したタケ培養細胞によるスチレン誘導体の生産2020

    • Author(s)
      北岡直樹、早田朋代、野村泰治、荻田信二郎、加藤康夫
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Pseudomonas putida由来enoyl-CoA hydratase/ aldolaseを導入したタケ培養細胞の作出2019

    • Author(s)
      北岡直樹、中畑未来、野村泰治、荻田信二郎、加藤康夫
    • Organizer
      日本農芸化学会 2019年度大会
    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] ヒドロキシ安息香酸誘導体の生合成方法2019

    • Inventor(s)
      北岡直樹、野村泰治、加藤康夫
    • Industrial Property Rights Holder
      富山県
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-038992
    • Filing Date
      2019
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2021-02-19  

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