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Estimating adaptive differentiation between two varieties of the genus Thujopsis to Pacific side / Japan Sea side climate based on cold tolerance characteristics

Research Project

Project/Area Number 18K14494
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 40010:Forest science-related
Research InstitutionForest Research and Management Organization

Principal Investigator

Inanaga Michiko  国立研究開発法人森林研究・整備機構, 森林総合研究所 林木育種センター, 主任研究員 等 (30757951)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywordsアスナロ / ヒノキアスナロ / 産地試験 / 低温順化 / 発現解析 / 糖分析 / アスナロ属 / 電解質漏出法 / RNA-seq / LT50 / 耐寒性 / 遺伝子発現
Outline of Final Research Achievements

Genus Thujopsis is endemic to Japan and consists of two varieties: T. dolabrata var. dolabrata, which is distributed mainly on the Pacific side, and T. dolabrata var. hondae, which is distributed mainly on the Sea of Japan side. Testing the hypothesis that there is a difference in winter cold tolerance between the two varieties, no difference was detected from November to early March during cold acclimation, thus rejecting the initial hypothesis. On the other hand, T. dolabrata var. dolabrata presented slightly higher cold tolerance in September-October and late March, suggesting that local adaptation to the provenance may occur at the preacclimation and the deacclimation stage. Sugar content analysis did not show any difference among the provenances. In addition, a T. dolabrata cDNA database with detailed annotations was developed for future use of comparison by expressed genes.

Academic Significance and Societal Importance of the Research Achievements

冬季の耐寒性と糖含有量に変種間差が見られなかったことから、両変種の分化は冬季の低温に対する適応に起因するという仮説は否定された。一方、低温に順化していない期間の耐寒性にわずかな差が見られたことから、アスナロ属の変種の分化には仮説と異なる環境要因への適応、もしくは集団の隔離等の歴史的経緯が関わっている可能性が示された。本研究で整備されたcDNAデータベースは、今後多様なアスナロ属の遺伝学的研究、ひいては育種等に応用することができる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report

Research Products

(7 results)

All 2021 2020 2018

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Genetic Diversity and Structure of Japanese Endemic Genus Thujopsis (Cupressaceae) Using EST-SSR Markers2020

    • Author(s)
      Inanaga Michiko、Hasegawa Yoichi、Mishima Kentaro、Takata Katsuhiko
    • Journal Title

      Forests

      Volume: 11 Pages: 935-935

    • DOI

      10.3390/f11090935

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] クロロフィル蛍光測定によるアスナロ属(genus Thujopsis)植物群の耐凍性獲得過程の推定2020

    • Author(s)
      稲永路子、高田克彦
    • Journal Title

      秋田県立大学ウェブジャーナルA(地域貢献と研究成果)

      Volume: 8 Pages: 25-31

    • NAID

      120006894832

    • Related Report
      2020 Annual Research Report
    • Open Access
  • [Presentation] アスナロ葉組織の低温順化過程における耐凍性および糖含有量の変化2021

    • Author(s)
      稲永路子、鈴木伸吾、工藤尚美、古賀泰雅、遠藤圭太、荒川圭太
    • Organizer
      第68回日本生態学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] アスナロ属葉組織における耐凍性関連遺伝子の発現解析2021

    • Author(s)
      稲永路子、平尾知士、高田克彦
    • Organizer
      第132回日本森林学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 2産地に由来するアスナロの低温順化過程における耐凍性の変化の比較2020

    • Author(s)
      稲永路子、遠藤圭太
    • Organizer
      第67回日本生態学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] アスナロ属の耐凍性関連遺伝子のプライマー開発2020

    • Author(s)
      稲永路子、平尾知士、高田克彦
    • Organizer
      第131回日本森林学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] RNA-seq analysis and de novo transcriptome assembly for detecting cold acclimation related genes in Thujopsis dolabrata var. Hondae2018

    • Author(s)
      INANAGA Michiko, TAKATA Katsuhiko
    • Organizer
      6th Plant Dormancy Symposium 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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