Mechanisms of glycogen utilization in fish embryos and production of stress-resistant eggs

• Project/Area Number: 18K14524
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 40040:Aquatic life science-related
• Research Institution: Kitasato University
• Principal Investigator: Furukawa Fumiya 北里大学, 海洋生命科学部, 講師 (80750281)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: グリコーゲン / 発生 / 糖質 / 代謝 / ゼブラフィッシュ / 胚 / 卵形成 / 糖 / 魚類 / 糖代謝

Outline of Final Research Achievements

We studied glycogen metabolism in developing zebrafish and other animals. In the zebrafish, glycogen was synthesized in the oocytes, and after fertilization, this polysaccharide was degraded by the embryos. Under hypoxic conditions, the embryogenesis and glycogen consumption were inhibited. To clarify the role of glycogen, we are trying to produce glycogen synthase-deficient zebrafish by the CRISPR/Cas9 system.
Masu salmon and Pacific abalone were also studied for comparison. These animals synthesized glycogen during early development, and then consumed the glycogen during hatching and settling, respectively.

Academic Significance and Societal Importance of the Research Achievements

持続可能な水産業の推進のためにも、完全養殖のカギを握る種苗生産過程における胚や仔魚のへい死を減らす必要がある。これまで、卵質に関わる物質が様々に研究されているが、実際に胚や仔魚の体内で起こる生理的な現象を理解する試みに乏しい。本研究では、細胞内貯蔵糖質として働くグリコーゲンについて検討した。グリコーゲンは卵内に貯蔵されることから、母親の飼育環境の調節により卵への貯蔵量を人為的に調節でき、卵質に影響を与えることができると推察される。本研究でグリコーゲンの蓄積・利用過程がある程度見えてきたので、今後は種苗生産への応用を見据え、グリコーゲンの生理的な役割を決定していく必要がある。

Research Products

• [Int'l Joint Research] Academia Sinica, Taiwan ROC(その他の国・地域)
• [Int'l Joint Research] ICOB/Academia Sinica/Taiwan(その他の国・地域)
• [Int'l Joint Research] Marine Research Station/Academia Sinica/Taiwan(その他の国・地域)
• [Int'l Joint Research] Academia Sinica(その他の国・地域)
• [Journal Article] Gluconeogenesis and glycogen metabolism during development of Pacific abalone, Haliotis discus hannai (2020)
  • Author(s): Koyama Mugen、Furukawa Fumiya、Koga Yuka、Funayama Shohei、Furukawa Suehiro、Baba Otto、Lin Ching-Chun、Hwang Pung-Pung、Moriyama Shunsuke、Okumura Sei-ichi
  • Journal Title: American Journal of Physiology-Regulatory, Integrative and Comparative Physiology Volume: 318 Issue: 3 Pages: R619-R633
  • DOI: 10.1152/ajpregu.00211.2019
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Changes in glycogen concentration and gene expression levels of glycogen-metabolizing enzymes in muscle and liver of developing masu salmon (2018)
  • Author(s): Fumiya Furukawa, Shotaro Irachi, Mugen Koyama, Otto Baba, Hajime Akimoto, Sei-ichi Okumura, Hirohiko Kagawa, Katsuhisa Uchida
  • Journal Title: Comparative Biochemistry and Physiology, Part A Volume: 225 Pages: 74-82
  • DOI: 10.1016/j.cbpa.2018.07.003
  • Peer Reviewed
• [Presentation] ゼブラフィッシュ卵黄多核層における糖新生 (2019)
  • Author(s): 青柳晶大、古川史也、安元剛、佐野香織、神保充、Tseng YC、Lin CC、内田勝久、Hwang PP
  • Organizer: 日本動物学会第90回大会
• [Presentation] Glycogen metabolism in the liver and muscle of developing salmonid fish, Oncorhynchus masou masou (2018)
  • Author(s): Fumiya Furukawa, Shotaro Irachi, Mugen Koyama, Otto Baba, Hajime Akimoto, Sei-ichi Okumura, Hirohiko Kagawa, Katsuhisa Uchida
  • Organizer: 13th International Congress on the Biology of Fish
  • Int'l Joint Research
• [Presentation] Glucose and glycogen metabolism in developing Pacific abalone, Haliotis discus hannai (2018)
  • Author(s): Mugen Koyama, Fumiya Furukawa, Yuka Koga, Shunsuke Moriyama, Sei-ichi Okumura
  • Organizer: 13th International Congress on the Biology of Fish
  • Int'l Joint Research