• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of basic technologies for developmental engneering using transplantation of spermatogenic stem cells in chicken

Research Project

Project/Area Number 18K14570
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 42010:Animal production science-related
Research InstitutionHiroshima University

Principal Investigator

Nakamura Yoshiaki  広島大学, 統合生命科学研究科(生), 助教 (30613723)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsニワトリ / マウス / 始原生殖細胞 / 精子幹細胞 / 移植 / 発生工学
Outline of Final Research Achievements

This study was conducted to improve research infrastructure in the field of chicken developmental engineering using transplantation of spermatogonial stem cells (SSCs). Using chicken as a model, an effective method for removal of endogenous germ cells was developed. The feasibility of this method for chicken SSC transplantation are to be evaluated in near future but is available for transplantation of primordial germ cells, progenitor cells for SSCs. To establish a strategy to improve SSC transplantation, fate behavior of individual SSCs were analyzed in mouse. As a result, transplanted SSCs follow stochastic fate and a major fraction of them were lost through differentiation and cell death. Based on this finding, SSC transplantation efficiency was dramatically improved by inhibiting differentiation of transplanted SSCs. To apply this strategy to chicken SSC transplantation, an approach to inhibit differentiation of chicken SSCs to be explored in near feature.

Academic Significance and Societal Importance of the Research Achievements

マウスで開発された精子幹細胞の移植法は、遺伝的多様性の保全や男性不妊治療への応用が期待されている。ニワトリにおいても精子幹細胞移植の成功例はあるが、実用レベルには達していない。本研究で開発したニワトリ生殖細胞の効率的な除去法は、精子幹細胞の移植に利用可能か検討中であるものの、始原生殖細胞の移植には利用可能であった。また、マウスで確立した精子幹細胞の移植効率を改善する方法論は、定説を覆すものであり、特にがん治療後の妊孕性回復へ貢献することが期待される。本研究では、この方法論がニワトリ精子幹細胞移植へ応用できるか結論を出すに至らなかったため、今後多角的なアプローチで検討を進める必要がある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (7 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Int'l Joint Research] University of Cambridge(英国)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Transient suppression of transplanted spermatogonial stem cell differentiation restores fertility in mice2021

    • Author(s)
      Nakamura Y, Jorg DJ, Kon Y, Simons BD, Yoshida S
    • Journal Title

      Cell Stem Cell

      Volume: 28 Issue: 8 Pages: 1-14

    • DOI

      10.1016/j.stem.2021.03.016

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ニワトリ始原生殖細胞に適した凍結保存液の開発2021

    • Author(s)
      濵井奈津子, 小出茅洋, 都築政起, 中村隼明
    • Organizer
      日本畜産学会第128回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 宿主精巣に移植した精子幹細胞はストカスティックな運命を辿る2020

    • Author(s)
      中村隼明, David J Jorg, Benjamin D Simons, 吉田松生
    • Organizer
      第113回日本繁殖生物学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Impact of KnockOut Serum Replacement in the culture of chicken primordial germ cells2019

    • Author(s)
      Chihiro Koide, Natsuko Hamai, Effrosyni Fatira, Sigeru Nomura, Atsushi Takenouchi, Masaoki Tsudzuki, Yoshiaki Nakamura
    • Organizer
      日本畜産学会第126回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ニワトリ始原生殖細胞の緩慢凍結に用いる凍結保護剤の比較選定2019

    • Author(s)
      濵井奈津子, 小出茅洋, 野村茂, 竹之内惇, 都築政起, 中村 隼明
    • Organizer
      日本畜産学会第126回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 家禽類および齧歯類における生殖細胞の移植を用いたキメラ作製技法2018

    • Author(s)
      中村隼明
    • Organizer
      第111回日本繁殖生物学会大会
    • Related Report
      2018 Research-status Report
    • Invited

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi