Development of animal model of Zika virus infection and identification of Zika virus receptors
Project/Area Number |
18K14599
|
Research Category |
Grant-in-Aid for Early-Career Scientists
|
Allocation Type | Multi-year Fund |
Review Section |
Basic Section 42020:Veterinary medical science-related
|
Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
Taniguchi Satoshi 国立感染症研究所, ウイルス第一部, 主任研究官 (60734465)
|
Project Period (FY) |
2018-04-01 – 2021-03-31
|
Project Status |
Completed (Fiscal Year 2020)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
Keywords | モデル動物 / ジカウイルス / 妊娠 / マーモセット / 動物モデル / CRISPR / CRISPR-Cas9 / in silico解析 |
Outline of Final Research Achievements |
A widespread epidemic of Zika fever, caused by Zika virus, occurred in South America in 2016. The evidence that Zika virus infection to pregnant woman can cause microcephaly in the fetus has been reported. Besides, it has been reported that Zika virus infection in adults can result in Guillain-Barre syndrome. To understand Zika virus pathogenicity, non-human primate pregnant animal model using common marmoset was established and analyzed. The results suggested that Zika virus infection caused miscarriage to maternal marmosets in first trimester pregnancy and Zika virus propagated in organs such as uterus and placenta. Besides, Cas9-expressing cell lines were established to analyze the entry mechanism of Zika virus. These cell lines will help us to identify new entry receptors of Zika virus and other viruses in the future study.
|
Academic Significance and Societal Importance of the Research Achievements |
マウスを用いたジカウイルスの妊娠感染モデルは多く報告されているが、より詳細な解析を行う上では、よりヒトに近い霊長類の妊娠感染モデルの確立が好ましい。旧世界ザルを用いた感染モデルの報告もあるが、一度に多くの感染動物を扱えないこと、妊娠期間が長期に及ぶこと、妊娠適齢個体を用意することの難しさから適切な動物モデルとは言い難い。その点、マーモセットは小型で取り扱いが容易であり、妊娠期間も他の霊長類と比較して短いことからマーモセットを用いたジカウイルスの妊娠感染モデルが確立されたことは意義深い。また、様々な種のCas-9恒常発現細胞を作製したことは今後のウイルス受容体研究に非常に役に立つと考えている。
|
Report
(4 results)
Research Products
(13 results)
-
[Journal Article] Dengue Virus Serotype 1 Exported to Japan from Côte d’Ivoire, 20192021
Author(s)
Suzuki Tetsuya、Kutsuna Satoshi、Nakamoto Takato、Ota Masayuki、Ishikane Masahiro、Yamamoto Kei、Maeki Takahiro、Tajima Shigeru、Nakayama Eri、Taniguchi Satoshi、Lim Chang-Kweng、Saijo Masayuki、Ohmagari Norio
-
Journal Title
Japanese Journal of Infectious Diseases
Volume: 74
Issue: 2
Pages: 148-150
DOI
NAID
ISSN
1344-6304, 1884-2836
Year and Date
2021-03-31
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Characterization of pseudotyped vesicular stomatitis virus bearing the heartland virus envelope glycoprotein.2021
Author(s)
Kimura M, Egawa K, Ozawa T, Kishi H, Shimojima M, Taniguchi S, Fukushi S, Fujii H, Yamada H, Tan L, Sano K, Katano H, Suzuki T, Morikawa S, Saijo M, Tani H.
-
Journal Title
Virology
Volume: 556
Pages: 124-132
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
[Journal Article] Increased Growth Ability and Pathogenicity of American- And Pacific-subtype Zika Virus (ZIKV) Strains Compared With a Southeast Asian-subtype ZIKV Strain2019
Author(s)
Yasuhiro Kawai, Eri Nakayama, Kenta Takahashi, Satoshi Taniguchi, Ken-Ichi Shibasaki, Fumihiro Kato, Takahiro Maeki, Tadaki Suzuki, Shigeru Tajima, Masayuki Saijo, Chang-Kweng Lim.
-
Journal Title
PLoS Neglected Tropical Diseases
Volume: 13
Issue: 6
Pages: e0007387-e0007387
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Presentation] Evaluation of vertical infection of pregnant marmosets with Zika virus2020
Author(s)
Satoshi Taniguchi, Chang-Kweng Lim, Takahiro Maeki, Shigeru Tajima, Eri Nakayama, Fumihiro Kato, Nor Azila Muhammad Azami, Yasushi Ami, Tadaki Suzuki, Meng Ling Moi, Yuriko Suzaki, Noriyo Nagata, Naoko Iwata, Tomohiko Takasaki, Ichiro Kurane, Masayuki Saijo
Organizer
U.S.-Japan Cooperative Medical Sciences Program (USJCMSP) 22nd International Conference on Emerging Infectious Diseases in the Pacific Rim
Related Report
Int'l Joint Research
-
-
-