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Establishment of cell polarity by interactions between Wnt and PCP factors.

Research Project

Project/Area Number 18K14720
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 44020:Developmental biology-related
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Mii Yusuke  基礎生物学研究所, 分子発生学研究部門, 助教 (70634129)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsXenopus / アフリカツメガエル / Wnt / モルフォゲン / 平面細胞極性 / PCP / イメージング / 超解像イメージング / ヘパラン硫酸
Outline of Final Research Achievements

Planar cell polarity is the directionaly of cells within a tissue plain, which is widely conserved among animals. A family of secreted signaling proteins, Wnt has been considered as a directional cue for PCP, supposedly by forming a concentration gradient as a morphogen. However, this model is not directly examined because visualization of Wnt ligands are still lacking. In this study, we found that a subtype of Wnt proteins involved in PCP did not show graded distributions, based on visualization of Wnt proteins. Furthermore, we found that core PCP components, which are essential for PCP formation, are involved in the distribution of the Wnt protein.

Academic Significance and Societal Importance of the Research Achievements

これまで、分泌性のシグナル蛋白質であるWntは濃度勾配によって位置情報を与える物質「モルフォゲン」の一種と捉えられており、この文脈にそって、組織平面内の細胞の方向性である「平面細胞極性」もWntの濃度勾配によって向きが揃えられていると考えられてきた。しかし、本研究からWnt蛋白質の分布が必ずしも濃度勾配的ではないことが判明し、さらに平面細胞極性の必須因子であるコアPCP因子はWntの分布に関与することが示された。これは従来のモルフォゲンの範疇にとどまらないWntの作用機序に対する理解を大きく前進させるものである。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (26 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (9 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 8 results,  Open Access: 9 results) Presentation (10 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Remarks (4 results)

  • [Int'l Joint Research] University of Ulsan College of Medicine(韓国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Biozentrum, University of Basel(スイス)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] The University of Hong Kong(中国)

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Quantitative analyses reveal extracellular dynamics of Wnt ligands in Xenopus embryos2021

    • Author(s)
      Mii Yusuke、Nakazato Kenichi、Pack Chan-Gi、Ikeda Takafumi、Sako Yasushi、Mochizuki Atsushi、Taira Masanori、Takada Shinji
    • Journal Title

      eLife

      Volume: 10 Pages: 55108-55108

    • DOI

      10.7554/elife.55108

    • NAID

      120007041648

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7-mediated Vangl ubiquitination and endoplasmic reticulum-associated degradation2021

    • Author(s)
      Feng Di、Wang Jin、Yang Wei、Li Jingyu、Lin Xiaochen、Zha Fangzi、Wang Xiaolu、Ma Luyao、Choi Nga Ting、Mii Yusuke、Takada Shinji、Huen Michael S. Y.、Guo Yusong、Zhang Liang、Gao Bo
    • Journal Title

      Science Advances

      Volume: 7 Issue: 20 Pages: 2099-2099

    • DOI

      10.1126/sciadv.abg2099

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc2021

    • Author(s)
      Matsuda Shinya、Schaefer Jonas V.、Mii Yusuke、Hori Yutaro、Bieli Dimitri、Taira Masanori、Pluckthun Andreas、Affolter Markus
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 6435-6435

    • DOI

      10.1038/s41467-021-26726-6

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis2021

    • Author(s)
      Yamamoto Kei、Miura Haruko、Ishida Motohiko、Mii Yusuke、Kinoshita Noriyuki、Takada Shinji、Ueno Naoto、Sawai Satoshi、Kondo Yohei、Aoki Kazuhiro
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 1-13

    • DOI

      10.1038/s41467-021-27458-3

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Heparan Sulfate Clusters Regulate Distribution and Signaling of Wnt Morphogens2020

    • Author(s)
      Mii Yusuke
    • Journal Title

      Trends in Glycoscience and Glycotechnology

      Volume: 32 Issue: 190 Pages: E205-E211

    • DOI

      10.4052/tigg.2006.7E

    • NAID

      130007942381

    • ISSN
      0915-7352, 1883-2113
    • Year and Date
      2020-11-25
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Heparan Sulfate Proteoglycan Clustering in Wnt Signaling and Dispersal2020

    • Author(s)
      Mii Yusuke、Takada Shinji
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 8 Pages: 631-631

    • DOI

      10.3389/fcell.2020.00631

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics2020

    • Author(s)
      Uda Youichi、Miura Haruko、Goto Yuhei、Yamamoto Kei、Mii Yusuke、Kondo Yohei、Takada Shinji、Aoki Kazuhiro
    • Journal Title

      ACS Chemical Biology

      Volume: 15 Issue: 11 Pages: 2896-2906

    • DOI

      10.1021/acschembio.0c00477

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Quantitative analyses reveal extracellular dynamics of Wnt ligands in Xenopus embryos2020

    • Author(s)
      Mii Yusuke、Nakazato Kenichi、Pack Chan-Gi、Sako Yasushi、Mochizuki Atsushi、Takada Shinji、Taira Masanori
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2020.02.20.957860

    • NAID

      120007041648

    • Related Report
      2019 Research-status Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Assembly of protein complexes restricts diffusion of Wnt3a proteins2018

    • Author(s)
      Takada, R., Mii, Y., Krayukhina, E., Maruyama, Y., Mio, K., Sasaki, Y., Shinkawa, T., Pack, C.-G., Sako, Y., Sato, C., Uchiyama, S., Takada, S.
    • Journal Title

      Commun. Biol.

      Volume: 1 Issue: 1 Pages: 165-165

    • DOI

      10.1038/s42003-018-0172-x

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Dynamic exchange of abundant scaffold-bound and rare freely diffusing ligands forms Wnt gradient.2021

    • Author(s)
      Mii Yusuke、Nakazato Kenichi、Pack Chan-Gi、Ikeda Takafumi、Sako Yasushi、Mochizuki Atsushi、Taira Masanori、Takada Shinji
    • Organizer
      日本発生生物学会第54回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Feedback regulation among heparan sulfate proteoglycans, Wnt11, and core PCP components are involved in organization of planar cell polarity.2021

    • Author(s)
      Minako Suzuki, Shinji takada, Yusuke Mii
    • Organizer
      日本発生生物学会第54回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Wnt11とコアPCP因子の相互的制御による平面細胞極性(PCP)の形成2021

    • Author(s)
      三井 優輔
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Mutual interactions of Wnt11 and core PCP components organize PCP and spatial distribution of Wnt112021

    • Author(s)
      Yusuke Mii
    • Organizer
      遺伝研研究会 Wnt研究会2021
    • Related Report
      2020 Research-status Report
  • [Presentation] Wntによる平面細胞極性の方向づけの分子メカニズム2020

    • Author(s)
      三井優輔、高田律子、松山誠、高田慎治
    • Organizer
      第53回日本発生生物学会大会(誌上開催)
    • Related Report
      2020 Research-status Report
  • [Presentation] 平面細胞極性におけるWnt11の局在化メカニズムの解析2020

    • Author(s)
      鈴木美奈子、高田慎治、三井優輔
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] Local and mutual regulations between Wnt and planar cell polarity components propagate global coordination of the planar cell polarity.2019

    • Author(s)
      三井優輔、高田律子、松山誠、高田慎治
    • Organizer
      第52回日本発生生物学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Two types of heparan sulfate clusters differently regulate Wnt distribution and signaling in Xenopus embryos.2019

    • Author(s)
      Yusuke Mii, Takayoshi Yamamoto, Ritsuko Takada, Shuji Mizumoto, Makoto Matsuyama, Shuhei Yamada, and Masanori Taira & Shinji Takada
    • Organizer
      11th International Conference on Proteoglycans
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] How does Wnt11 direct the planar cell polarity?2019

    • Author(s)
      Yusuke Mii
    • Organizer
      119th International Titisee Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Local and mutual regulations between Wnt and planar cell polarity propagate global coordination of cell polarity throughout the tissue.2018

    • Author(s)
      三井 優輔
    • Organizer
      第70回日本細胞生物学会・第51回日本発生生物学会合同大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Remarks] 分子発生学研究部門

    • URL

      https://www.nibb.ac.jp/cib2/

    • Related Report
      2019 Research-status Report
  • [Remarks] researchmap 三井 優輔

    • URL

      https://researchmap.jp/miiy

    • Related Report
      2019 Research-status Report
  • [Remarks] 分子発生学研究部門

    • URL

      http://www.nibb.ac.jp/sections/developmental_biology/takada/

    • Related Report
      2018 Research-status Report
  • [Remarks] 分子発生学研究部門(高田研究室)

    • URL

      http://www.nibb.ac.jp/cib2/

    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2024-12-25  

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