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Live-imaging analysis of root hairs contacting with the soil-mimicked obstacles

Research Project

Project/Area Number 18K14730
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionNational Institute for Basic Biology (2019)
Nagoya University (2018)

Principal Investigator

Shikata Hiromasa  基礎生物学研究所, 植物環境応答研究部門, 助教 (10813272)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords根毛 / 土壌粒子 / マイクロ流路 / 接触応答 / 機械刺激 / 脂質 / キナーゼ / 生細胞イメージング / 接触刺激 / 極性成長 / 土壌環境
Outline of Final Research Achievements

Root hairs in plants facilitate to absorb water and nutrients from soils. Morphology and growth of root hairs have been analyzed with plants conventionally grown on solidified media or in liquid media where root hairs can grow without space limitation. However, soils are filled with the particles like sands and silt, which could restrict root hair growth. To get insight into the root hairs growth in soils, we have established an imaging system to observe the contact between growing root hairs and obstacles. By this system, we revealed the dynamics in the root hair cells contacting with obstacles.

Academic Significance and Societal Importance of the Research Achievements

根毛は根の表面に無数に生える非常に細い構造体であるが、個々が1つの細胞から形成されている。根毛を生やすことで根と土壌との接触面積を増やし、植物は土壌からの水分や養分の吸収を効率的に行っているとされている。土壌は様々な大きさの粒子によって構成されているが、根毛がそのような粒子の中をどのように成長するのかはよくわかっていない。そこで、本研究では根毛が土壌粒子とどのように接触し成長するのかについて明らかにするため、光を透過する物質を用いて障害物を構築することで、根毛と障害物との接触を顕微鏡下で観察することに成功した。今後、根毛を介した水分や養分の効率的な吸収の仕組み解明に貢献することが期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Products Report
  • Research Products

    (14 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (1 results)

  • [Int'l Joint Research] Technical University of Munich(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Institute of Experimental Botany, CAS(チェコ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ミュンヘン工科大学(ドイツ)

    • Related Report
      2018 Research-status Report
  • [Journal Article] 土壌中の植物の根系を生きたまま視るには2021

    • Author(s)
      四方 明格
    • Journal Title

      アグリバイオ

      Volume: 5 Pages: 59-61

    • NAID

      40022471307

    • Related Report
      Products Report
  • [Journal Article] Mass-spectrometry-based draft of the Arabidopsis proteome.2020

    • Author(s)
      Mergner, J., Frejno, M., List, M., Papacek, M., Chen, X., Chaudhary, A., Samaras, P., Richter, S., Shikata, H., et al.
    • Journal Title

      Nature

      Volume: 579 Issue: 7799 Pages: 409-414

    • DOI

      10.1038/s41586-020-2094-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 細胞膜上で動的な局在性を示すAGCキナーゼによるシロイヌナズナ根毛の細胞伸長方向の制御2021

    • Author(s)
      四方 明格, Martina Kolb, Ulrich Hammes, 柳沢 直樹, 佐藤 良勝, 東山 哲也, Claus Schwechheimer
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      Products Report
  • [Presentation] Distribution of two phospholipids defines a dynamic plasma membrane domain for re-orientation of root hair tip growth2021

    • Author(s)
      Hiromasa Shikata
    • Organizer
      IRN France-Japan Frontiers in Plant Biology
    • Related Report
      Products Report
    • Int'l Joint Research
  • [Presentation] Distribution of two phospholipids specifies a dynamic plasma membrane domain for re-orientation of root hair tip growth.2020

    • Author(s)
      Shikata, H., Yanagisawa,N., Sato, Y., Higashiyama, H., Schwechheimer, C.
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 根毛の迅速かつ柔軟な伸長におけるリン脂質・細胞骨格動態2020

    • Author(s)
      四方 明格
    • Organizer
      植物細胞骨格研究会2020
    • Related Report
      Products Report
  • [Presentation] Live-imaging of Arabidopsis roots and root hairs by a tiny hydroponic system.2019

    • Author(s)
      Shikata, H.
    • Organizer
      International Meeting for Young Researchers “Frontiers in Imaging Probes and Technologies”
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 根毛において極性局在するAGCキナーゼによる細胞伸長調節2019

    • Author(s)
      四方 明格
    • Organizer
      第8回エンドメンブレンミーティング(JANPER2019)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 染色や薬剤処理に適した簡便なシロイヌナズナ根および根毛のライブイメージング系の構築2018

    • Author(s)
      四方 明格,佐藤 良勝,Claus Schwechheimer
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 根毛の伸長方向制御機構の解析:障害物との接触における根毛の成長ダイナミクス2018

    • Author(s)
      四方明格、柳沢直樹、佐藤良勝、東山哲也、Claus Schwechheimer
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Remarks] https://researchmap.jp/hiromasa_shikata/

    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-04-15  

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