Analysis of mechanisms underlying protein degradation enabling protein localization in response to gravity.
| Project/Area Number |
18K14731
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 44030:Plant molecular biology and physiology-related
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
Nakamura Moritaka 基礎生物学研究所, 植物環境応答研究部門, 特任研究員 (90808560)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 重力屈性 / 重力シグナリング / LAZY1ファミリー / タンパク質分解 / 細胞内局在 / シグナル伝達 |
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| Outline of Final Research Achievements |
Gravity signaling is an important process that controls directional auxin transport following amyloplasts relocation in gravitropism. In Arabidopsis, LAZY1 family proteins are shown to play a key role in gravity signaling. However, it is still uncertain how LAZY1 family proteins are involved in gravity signaling possibly due to their low abundance or high turnover rate. Here, this study revealed that LZY3, a member of LAZY1 family, is ubiquitylated, suggesting that the abundance of LZY3 is regulated by the ubiquitin-proteasome pathway. In addition, high-sensitive live cell imaging technique successfully enabled the visualization of LZY3, despite its low abundance. Further imaging analysis revealed that LZY3 changes its localization toward the lower side of the cell following amyloplasts relocation in response to gravistimulation.
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| Academic Significance and Societal Importance of the Research Achievements |
これまでの研究では、LAZY1ファミリーの分子レベルでの研究は十分にされていなかった。また、LAZY1ファミリーが重力シグナリングで機能するにも関わらず、上流のプロセスであるアミロプラスト沈降との関連性に着目した研究は全くされていなかった。本研究により、LZY3はユビキチン化されることで存在量が低く保たれていることが明らかになり、重力シグナリングにおけるLAZY1ファミリーの量的制御の重要性を示すことができた。さらには、アミロプラスト動態とLZY3の細胞内動態を時空間的に関連づけたことにより、今後、LAZY1ファミリーを中心とした重力シグナリングの分子機構の理解が進むと期待できる。
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Report
(3 results)
Research Products
(8 results)
