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Functional analysis of genes related to early calcification processes of scleractinian corals

Research Project

Project/Area Number 18K14745
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 44040:Morphology and anatomical structure-related
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Yasuoka Yuuri  国立研究開発法人理化学研究所, 生命医科学研究センター, 研究員 (70724954)

Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords刺胞動物 / 変態 / バイオミネラリゼーション / 発生 / 進化 / 地球温暖化 / 海洋酸性化 / PKS / 比較トランスクリプトーム / カルシウム骨格 / 環境ストレス / ミドリイシ
Outline of Final Research Achievements

To investigate functions of coral calcification-related genes, antisense morpholino oligos for gene knockdown ware microinjected to Acropora tenuis fertilized eggs. However, no phenotypes regarding the calcification process were observed in morphants. In addition, to examine temperature and pH sensitivity of coral embryos, A. tenuis and A. sp.1 embryos were developed and metamorphosed in different conditions. The results showed that A. tenuis embryos cannot survive after 3 hours incubation at 16°C or 21°C, and that A. tenuis and A. sp.1 embryos and larvae are tolerant to high temperature (~31°C) and low pH (pH7.15). Furthermore, transcriptome analyses using early polyps of A. tenuis and A. sp.1 were performed. New candidate genes that regulate coral calcification would be identified by clustering analysis.

Academic Significance and Societal Importance of the Research Achievements

本研究の環境変動実験の結果、地球温暖化や海洋酸性化といった現代人類が直面している地球環境問題に対して、少なくともA. tenuisは正常に胚発生・着底して石灰化を始められるだけの頑健性を備えていることが示唆された。また、5-6月に産卵する通常のミドリイシと異なり、8-9月に産卵するAcropora sp.1のトランスクリプトーム解析を行ったことで、種を超えて保存された生命現象の解明や、産卵時期の変更に関わった遺伝子の同定など、今後のサンゴ研究に向けたデータ基盤が構築された。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (39 results)

All 2023 2022 2021 2020 2019 2018 Other

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (25 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Tissue-specific expression of carbohydrate sulfotransferases drives keratan sulfate biosynthesis in the notochord and otic vesicles of Xenopus embryos2023

    • Author(s)
      Yasuoka Yuuri
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 11 Pages: 957805-957805

    • DOI

      10.3389/fcell.2023.957805

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Uncovering the mesendoderm gene regulatory network through multi-omic data integration2022

    • Author(s)
      Jansen Camden、Paraiso Kitt D.、Zhou Jeff J.、Blitz Ira L.、Fish Margaret B.、Charney Rebekah M.、Cho Jin Sun、Yasuoka Yuuri、Sudou Norihiro、Bright Ann Rose、Wlizla Marcin、Veenstra Gert Jan C.、Taira Masanori、Zorn Aaron M.、Mortazavi Ali、Cho Ken W.Y.
    • Journal Title

      Cell Reports

      Volume: 38 Issue: 7 Pages: 110364-110364

    • DOI

      10.1016/j.celrep.2022.110364

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] LIM homeodomain proteins and associated partners: Then and now2021

    • Author(s)
      Yasuoka Yuuri、Taira Masanori
    • Journal Title

      Current Topics in Developmental Biology

      Volume: 145 Pages: 113-166

    • DOI

      10.1016/bs.ctdb.2021.04.003

    • ISBN
      9780128168332
    • Related Report
      2021 Research-status Report
  • [Journal Article] Morphogenetic mechanisms forming the notochord rod: The turgor pressure‐sheath strength model2020

    • Author(s)
      Yasuoka Yuuri
    • Journal Title

      Development, Growth & Differentiation

      Volume: 00 Issue: 6 Pages: 1-12

    • DOI

      10.1111/dgd.12665

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evolution of cis-regulatory modules for the head organizer gene goosecoid in chordates: comparisons between Branchiostoma and Xenopus2019

    • Author(s)
      Yasuoka Yuuri、Tando Yukiko、Kubokawa Kaoru、Taira Masanori
    • Journal Title

      Zoological Letters

      Volume: 5 Issue: 1 Pages: 1-17

    • DOI

      10.1186/s40851-019-0143-1

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evolutionary History of GLIS Genes Illuminates Their Roles in Cell Reprograming and Ciliogenesis2019

    • Author(s)
      Yasuoka Yuuri、Matsumoto Masahito、Yagi Ken、Okazaki Yasushi
    • Journal Title

      Molecular Biology and Evolution

      Volume: 37 Issue: 1 Pages: 100-109

    • DOI

      10.1093/molbev/msz205

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] De novo transcription of multiple Hox cluster genes takes place simultaneously in early Xenopus tropicalis embryos2019

    • Author(s)
      Kondo Mariko、Matsuo Megumi、Igarashi Kento、Haramoto Yoshikazu、Yamamoto Takayoshi、Yasuoka Yuuri、Taira Masanori
    • Journal Title

      Biology Open

      Volume: 8

    • DOI

      10.1242/bio.038422

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microinjection of DNA Constructs into Xenopus Embryos for Gene Misexpression and cis-Regulatory Module Analysis2018

    • Author(s)
      Yasuoka Yuuri、Taira Masanori
    • Journal Title

      Cold Spring Harbor Protocols

      Volume: 2019 Issue: 1 Pages: pdb.prot097279-pdb.prot097279

    • DOI

      10.1101/pdb.prot097279

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Molecular Basis of the Gastrula Organizer in Amphibians and Cnidarians2018

    • Author(s)
      Yasuoka Yuuri、Taira Masanori
    • Journal Title

      Reproductive and Developmental Strategies. Diversity and Commonality in Animals (Book)

      Volume: - Pages: 667-708

    • DOI

      10.1007/978-4-431-56609-0_31

    • ISBN
      9784431566076, 9784431566090
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 発生ゲノミクスで探る脊椎動物の進化過程2023

    • Author(s)
      安岡有理
    • Organizer
      生命誌 研究者セミナー
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] ゲノム・トランスクリプトーム・プロテオームから見たシロアゴガエルミトコンドリアゲノム進化の背景2023

    • Author(s)
      安岡有理
    • Organizer
      広島大学両生類研究センターバイオリソース棟落成記念シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] 動物胚初期発生における遺伝子制御ネットワークとその進化に関する研究2022

    • Author(s)
      安岡有理
    • Organizer
      日本動物学会第93回早稲田大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] ツメガエルを用いたミトコンドリアゲノム編集への挑戦ー第2章2022

    • Author(s)
      安岡有理、岡﨑康司
    • Organizer
      日本動物学会第93回早稲田大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 初期胚における遺伝子発現の揺らぎと進化の関係を探る2022

    • Author(s)
      安岡有理
    • Organizer
      第24回日本進化学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Fluctuation of gene expression levels in early embryos is correlated to environmental and evolutionary responses2022

    • Author(s)
      Yuuri Yasuoka, Chikara Furusawa, Yasushi Okazaki
    • Organizer
      第55回日本発生生物学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Comparing fluctuation of gene expression profiles in embryos to dissect diversification of Xenopus tropicalis strains2021

    • Author(s)
      Yuuri Yasuoka
    • Organizer
      18th International Xenopus Conference
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 遺伝子発現の多様性から制御ネットワーク進化の制約と方向性を探る2021

    • Author(s)
      安岡有理、古澤力、岡﨑康司
    • Organizer
      JST CREST バイオDX領域キックオフシンポジウム
    • Related Report
      2021 Research-status Report
  • [Presentation] ツメガエルを用いたミトコンドリアゲノム編集への挑戦~序章~2021

    • Author(s)
      安岡有理、岡﨑康司
    • Organizer
      日本動物学会第94回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 脊索の祖先的構成要素と形態形成様式の進化2021

    • Author(s)
      安岡有理
    • Organizer
      第23回日本進化学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] シロアゴガエルと疑似ロングリードシークエンス2021

    • Author(s)
      安岡有理
    • Organizer
      第14回日本ツメガエル研究集会
    • Related Report
      2021 Research-status Report
  • [Presentation] Evolutionary History of GLIS1 and GLIS3 Illuminates Their Roles in Cell Reprograming and Ciliogenesis2021

    • Author(s)
      Yuuri Yasuoka, Masahito Matsumoto, Ken Yagi, and Yasushi Okazaki
    • Organizer
      第54回日本発生生物学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Repeated subfunctionalization of Brachyury genes for notochord development (脊索の発生におけるBrachyury遺伝子の機能分担)2020

    • Author(s)
      安岡有理
    • Organizer
      日本進化学会第22回オンライン大会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 初期化因子GLIS1の進化的起源と祖先的役割2020

    • Author(s)
      安岡有理, 松本征仁, 八木研, 岡﨑康司
    • Organizer
      日本動物学会第91回大会(招待講演)
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Genome-wide Measurement for Fluctuation of Gene Regulatory Networks in Xenopus embryos2020

    • Author(s)
      Yuuri Yasuoka
    • Organizer
      JSDB Online Trial Meeting 2020
    • Related Report
      2020 Research-status Report
  • [Presentation] Genome-wide Measurement for Fluctuation of Gene Regulatory Networks in Xenopus embryos2020

    • Author(s)
      Yuuri Yasuoka
    • Organizer
      第53回日本発生生物学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] Genome-wide Measurement for Fluctuation of Gene Regulatory Networks in Xenopus embryos2019

    • Author(s)
      Yasuoka Yuuri
    • Organizer
      Xenopus Resources and Emerging Technologies Meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Why was the notail gene lost in many vertebrate lineages?2019

    • Author(s)
      Yasuoka Yuuri, Satoh Noriyuki
    • Organizer
      52nd Annual Meeting of JSDB
    • Related Report
      2019 Research-status Report
  • [Presentation] Evolutionary History of GLIS Genes Illustrates their Fundamental Roles in Cell Reprogramming and Ciliogenesis2019

    • Author(s)
      Yasuoka Yuuri, Matsumoto Masayuki, Yagi Ken, Okazaki Yasushi
    • Organizer
      21st Annual Meeting of the Society of Evolutionary Studies, Japan
    • Related Report
      2019 Research-status Report
  • [Presentation] Fluctuation of GRN in Xenopus tropicalis embryos2019

    • Author(s)
      Yasuoka Yuuri
    • Organizer
      12th Japan Xenopus Conference
    • Related Report
      2019 Research-status Report
  • [Presentation] Complex evolution of brachyury paralogs during vertebrate notochord development2018

    • Author(s)
      Yuuri Yasuoka and Noriyuki Satoh
    • Organizer
      第46回内藤コンファレンス(国際学会)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 脊椎動物の脊索発生におけるBrachyuryパラログの複雑な進化2018

    • Author(s)
      安岡有理、佐藤矩行
    • Organizer
      第20回日本進化学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 進化学の視点がリプログラミング研究にどう貢献できるか?2018

    • Author(s)
      安岡有理
    • Organizer
      第10回EvoDevo青年の会
    • Related Report
      2018 Research-status Report
  • [Presentation] ネッタイツメガエル胚発生における転写因子ー標的遺伝子関係の揺らぎ測定2018

    • Author(s)
      安岡有理
    • Organizer
      第12回XCIJ首都圏支部会
    • Related Report
      2018 Research-status Report
  • [Presentation] Keratan sulfate produces “water bags” in embryos2018

    • Author(s)
      Yuuri Yasuoka
    • Organizer
      第51回日本発生生物学会年会
    • Related Report
      2018 Research-status Report
  • [Book] ヒトゲノム事典2021

    • Author(s)
      井ノ上 逸朗, 今西 規, 河村 正二, 斎藤 成也, 颯田 葉子, 田嶋 敦
    • Total Pages
      448
    • Publisher
      一色出版
    • ISBN
      9784910389127
    • Related Report
      2021 Research-status Report
  • [Remarks] Yuuri Yasuoka, Ph. D.

    • URL

      https://yuuriyasuoka.wordpress.com/

    • Related Report
      2022 Annual Research Report
  • [Remarks] 応用ゲノム解析技術研究チーム

    • URL

      https://www.ims.riken.jp/labo/69/index_j.html

    • Related Report
      2020 Research-status Report
  • [Remarks] Research Map

    • URL

      https://researchmap.jp/yuuri/research_projects

    • Related Report
      2020 Research-status Report
  • [Remarks] 初期化因子の驚きの進化を解明 -リプログラミング研究に新たな視点を与える-

    • URL

      https://www.riken.jp/press/2019/20190913_3/index.html

    • Related Report
      2019 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2024-01-30  

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