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Ecology and evolution of hot-spring frogs

Research Project

Project/Area Number 18K14795
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 45040:Ecology and environment-related
Research InstitutionIwate Medical University

Principal Investigator

Komaki Shohei  岩手医科大学, いわて東北メディカル・メガバンク機構, 特命助教 (90789629)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Keywords両生類 / 熱ストレス / 遺伝子発現 / 繁殖行動 / 温度耐性 / 遺伝子発現解析 / 生態 / 温度適応 / 温泉 / リュウキュウカジカガエル / 行動 / 進化
Outline of Final Research Achievements

In this research, I focused on Buergeria frogs, a genus with an extraordinary characteristic of inhabiting hot springs, and studied underlying mechanisms that enable them to live in harsh, high-temperature environments through observational and transcriptome studies. Behavioral observations revealed that both tadpoles and adults prefer lower water temperatures for inhabiting and breeding. Gene expression analyses showed that genes involved in suppressive functions, such as global transcription and translation suppression and protein degradation, were differentially expressed in response to heat stress. These results suggest that while they can temporarily tolerate high thermal stress, they also can avoid long-term stress by temperature-selective behavior, making it possible to survive in hot spring environments.

Academic Significance and Societal Importance of the Research Achievements

温泉に生息するリュウキュウカジカガエルの幼生は最高で46℃の水域で発見されており、これは報告されている世界中のどの両生類よりも高温である。源泉付近は70℃を超える熱湯であり、極限的な生息環境といえる。このような環境で集団が維持できる要因を知ることは生物の環境適応の可能性を理解することにつながる進化生態学的意義をもつ。本研究で得られた知見はその一助となるだろう。
また、両生類における温度ストレスに注目した遺伝子発現解析は研究例が少なく、本研究のような高温ストレスを扱った研究は他に例がない。本研究で得られたデータや知見は両生類と熱ストレスの関係を理解するための基盤となる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] National Taiwan Normal University/National Museum of Natural Science/Endemic Species Research Institute(その他の国・地域)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Macquarie University(オーストラリア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 国立台湾師範大学(その他の国・地域)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Hot spring frogs (Buergeria japonica) prefer cooler water to hot water2020

    • Author(s)
      Komaki Shohei、Sutoh Yoichi、Kobayashi Kensuke、Saito Shigeru、Saito Claire T.、Igawa Takeshi、Lau Quintin
    • Journal Title

      Ecology and Evolution

      Volume: 10 Issue: 17 Pages: 9466-9473

    • DOI

      10.1002/ece3.6637

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Renaming a new Buergeria species from Taiwan - correction of a nomenclatural act not available to ICZN2020

    • Author(s)
      Wang Ying-Han, Hsiao Yu-Wei, Lee Ko-Huan, Tseng Hui-Yun, Lin Yen-Po, Komaki Shohei, Lin Si-Min
    • Journal Title

      Nature Conservation Quarterly

      Volume: 111 Pages: 4-13

    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ecoloy and evolution of hot spring frogs2020

    • Author(s)
      Shohei Komaki
    • Organizer
      The 9th World Congress of Herpetology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 温泉ガエルの生態学2020

    • Author(s)
      小巻翔平
    • Organizer
      コホート・生体試料支援プラットフォーム 若手支援研究成果発表会
    • Related Report
      2019 Research-status Report
  • [Presentation] 琉球列島の生物地理区境界2019

    • Author(s)
      小巻翔平
    • Organizer
      第64回日本生態学会東北地区会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 温泉ガエルの生態学2018

    • Author(s)
      小巻翔平
    • Organizer
      第4回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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