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Neuronal circuit regulation by the microglial mechanosensitivity

Research Project

Project/Area Number 18K14825
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 46010:Neuroscience-general-related
Research InstitutionNational Institute for Physiological Sciences

Principal Investigator

Horiuchi Hiroshi  生理学研究所, 基盤神経科学研究領域, 特任助教 (60760733)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsミクログリア / 力学知覚 / 神経回路 / 機械受容チャネル / 神経活動 / カルシウムイメージング / ATP
Outline of Final Research Achievements

Microglia are the main immune effector cells in the central nervous system (CNS) in the brain, actively motile their processes and monitor the brain status by contacting various cells. However, it has been unknown how microglia response to the mechanical stimulation. In this study, we visualized microglial functional activity using two-photon Ca imaging, revealed their response to the stimulation of the receptor. To investigate the physiological function, we generated microglia specific mechano-receptor deficient mice, investigated change of their process activity.

Academic Significance and Societal Importance of the Research Achievements

本研究では細胞の活動指標であるCa濃度の変化を可視化することによって、ミクログリアの活動様式を明らかにした。さらにこれを用いることで、ミクログリアが物理的刺激や機械受容体を介してどのように応答しているかを明らかにした。今後同受容体の機能制御によって、ミクログリアの活動変化やそれを介した生体機能への影響を見出し、ミクログリアの力学知覚とそれを介した生理機能を明らかにすることができれば、未知の生理機能の発見や物理信号を治療標的とした新たな治療戦略の構築へと結びつく可能性がある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (13 results)

All 2021 2020 2019

All Journal Article (8 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (5 results)

  • [Journal Article] Reactive astrocyte-driven epileptogenesis is induced by microglia initially activated following status epilepticus2021

    • Author(s)
      Sano Fumikazu、Shigetomi Eiji、Shinozaki Youichi、Tsuzukiyama Haruka、Saito Kozo、Mikoshiba Katsuhiko、Horiuchi Hiroshi、Cheung Dennis Lawrence、Nabekura Junichi、Sugita Kanji、Aihara Masao、Koizumi Schuichi
    • Journal Title

      JCI Insight

      Volume: - Issue: 9

    • DOI

      10.1172/jci.insight.135391

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Interleukin‐19 ameliorates experimental autoimmune encephalitis2021

    • Author(s)
      Takeuchi Hideyuki、Horiuchi Hiroshi、Parajuli Bijay、Komiya Hiroyasu、Tanaka Fumiaki、Suzumura Akio
    • Journal Title

      Clinical and Experimental Neuroimmunology

      Volume: - Issue: 3 Pages: 148-149

    • DOI

      10.1111/cen3.12642

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Interleukin-19 Abrogates Experimental Autoimmune Encephalomyelitis by Attenuating Antigen-Presenting Cell Activation2021

    • Author(s)
      Horiuchi Hiroshi、Parajuli Bijay、Komiya Hiroyasu、Ogawa Yuki、Jin Shijie、Takahashi Keita、Azuma Yasu-Taka、Tanaka Fumiaki、Suzumura Akio、Takeuchi Hideyuki
    • Journal Title

      Frontiers in Immunology

      Volume: 12 Pages: 615465-615465

    • DOI

      10.3389/fimmu.2021.615898

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Interleukin-19 alleviates experimental autoimmune encephalomyelitis by attenuating antigen-presenting cell activation2020

    • Author(s)
      Horiuchi Hiroshi、Parajuli Bijay、Komiya Hiroyasu、Ogawa Yuki、Shijie Jin、Takahashi Keita、Azuma Yasu-Taka、Tanaka Fumiaki、Suzumura Akio、Takeuchi Hideyuki
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2020.07.15.204826

    • Related Report
      2020 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] CMOS-based bio-image sensor spatially resolves neural activity-dependent proton dynamics in the living brain2020

    • Author(s)
      Horiuchi Hiroshi、Agetsuma Masakazu、Ishida Junko、Nakamura Yusuke、Lawrence Cheung Dennis、Nanasaki Shin、Kimura Yasuyuki、Iwata Tatsuya、Takahashi Kazuhiro、Sawada Kazuaki、Nabekura Junichi
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 712-712

    • DOI

      10.1038/s41467-020-14571-y

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Physiological Implications of Microglia-Synapse Interactions2019

    • Author(s)
      Wake Hiroaki、Horiuchi Hiroshi、Kato Daisuke、Moorhouse Andrew J.、Nabekura Junichi
    • Journal Title

      Methods Mol Biol.

      Volume: 2034 Pages: 69-80

    • DOI

      10.1007/978-1-4939-9658-2_6

    • ISBN
      9781493996575, 9781493996582
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] In Vivo Two-Photon Imaging of Microglial Synapse Contacts2019

    • Author(s)
      Kato Daisuke、Ikegami Ako、Horiuchi Hiroshi、Moorhouse Andrew J.、Nabekura Junichi、Wake Hiroaki
    • Journal Title

      Methods Mol Biol.

      Volume: 2034 Pages: 281-286

    • DOI

      10.1007/978-1-4939-9658-2_20

    • ISBN
      9781493996575, 9781493996582
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] ShadowR: a novel chromoprotein with reduced non-specific binding and improved expression in living cells2019

    • Author(s)
      Murakoshi Hideji、Horiuchi Hiroshi、Kosugi Takahiro、Onda Maki、Sato Aiko、Koga Nobuyasu、Nabekura Junichi
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 12072-12072

    • DOI

      10.1038/s41598-019-48604-4

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] イオンイメージセンサによる生体脳pHイメージング2021

    • Author(s)
      堀内 浩
    • Organizer
      【機能タンパク質の構造と機能のダイナミクスと、それに基づく細胞・生体システム作動機構の研究拠点の形成】シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] CMOSイメージセンサによる生体脳pHイメージング2020

    • Author(s)
      堀内 浩
    • Organizer
      名古屋大学大学院医学系研究科-生理学研究所合同シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] CMOS-based bio-image sensor spatially resolves neural activity dependent proton dynamics in the living brain2020

    • Author(s)
      Hiroshi Horiuchi, Masakazu Agetsuma, Junko Ishida, Dennis L Cheung, Kazuaki Sawada, Junichi Nabekura
    • Organizer
      ABiS Symposium Forefront and Future of Electron Microscopic Imaging
    • Related Report
      2019 Research-status Report
  • [Presentation] 覚醒下ミクログリアの2光子カルシウムイメージング2019

    • Author(s)
      堀内 浩,石田順子,鍋倉淳一
    • Organizer
      第66回中部日本生理学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 覚醒脳における神経活動依存的なプロトンダイナミクスを捉えるための新規CMOSバイオイメージセンサの開発2019

    • Author(s)
      石田順子、堀内 浩、揚妻正和、Dennis Cheung、澤田和明、鍋倉淳一
    • Organizer
      第8回生理研・名大医合同シンポジウム
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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