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Induction of cancer stem cell-like cells by endothelin 1 and prediction of cancer treatment using its receptor inhibitor

Research Project

Project/Area Number 18K15243
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 50010:Tumor biology-related
Research InstitutionOkayama University

Principal Investigator

Seno Akimasa  岡山大学, ヘルスシステム統合科学学域, 助教 (10759210)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywordsがん幹細胞 / エンドセリン1 / エンドセリン1 / iPS細胞 / ボセンタン / ヒトiPS細胞
Outline of Final Research Achievements

It has been shown that cancer stem cells can be induced by adding the culture supernatant of cancer cells to the culture medium of normal iPS cells and continuing the culture for a month. From the microarray data analysis results, endothelin 1 (ET1) was suggested as a substance among the secreted products of cancer cells, which induces iPS cells to become cancer stem cells. It was confirmed that ET1 is contained in the culture supernatant of cancer cells that could actually induce cancer stem cells, and the expression of the EDNRB gene, one of the endothelin receptors, was higher in cancer stem cells induced with the culture supernatant of cancer cells than in the original iPS cells. Moreover, when iPS cells were cultured with ET1, their tumorigenic potential was found to be enhanced compared to that of the original iPS cells.

Academic Significance and Societal Importance of the Research Achievements

がんの発症原因には様々なものがあるが、直接の原因は遺伝子変異により起こるとされることが多い。しかしながら、正常な末梢細胞が培養条件により生じることと同様にがん細胞もその分化にふさわしい培養条件があることを示す研究も存在している。本研究もそのような研究の1つであり、未だ全容が明らかとならないがんの発症原因究明の一助となることが期待される。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (3 results)

All 2019 Other

All Int'l Joint Research (1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] Wayne State University/Barbara Ann Karmanos Cancer Institute(米国)

    • Related Report
      2018 Research-status Report
  • [Presentation] The effect of endothelin 1 on cancer stem cell development2019

    • Author(s)
      Akimasa Seno, Ira Winer, Manohar Ratnam, and Masaharu Seno
    • Organizer
      American Association for Cancer Research Annual meeting 2019
    • Related Report
      2019 Research-status Report
  • [Presentation] The effect of endothelin 1 on cancer stem cell development2019

    • Author(s)
      Akimasa Seno, Ira Winer, Manohar Ratnam, and Masaharu Seno
    • Organizer
      American Association for Cancer Research Annual Meeting 2019
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2025-03-27  

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