Protocol development for detecting tiny pancreatic cancer using hyperpolarized DNP-MRI

Research Project

Project/Area Number	18K15312
Research Category	Grant-in-Aid for Early-Career Scientists
Allocation Type	Multi-year Fund
Review Section	Basic Section 50020:Tumor diagnostics and therapeutics-related
Research Institution	Gifu University
Principal Investigator	Noda Yoshifumi 岐阜大学, 医学部附属病院, 助教 (60643020)
Project Period (FY)	2018-04-01 – 2021-03-31
Project Status	Completed (Fiscal Year 2020)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	MRI / 膵癌
Outline of Final Research Achievements	MIA PaCa-2 cells were subcutaneously administered to the right leg of mice. In vivo DNP-MRI were performed at pre, 1, 3, 7 days after irradiation. Decay rate was calculated by the change in DNP image intensity. Redox map was obtained by the slope of the enhanced DNP image intensity using a custom Excel macro program DNP-MRI scanning of right leg was started immediately after intravenous injection of nitroxyl contrast agent, CmP. Pharmacokinetic DNP-MRI images were performed at 0.5, 2, 4, 6, 8, 10, 14, 18, and 22 minutes after administration. Decay rate was significantly decreased at day 1 after irradiation compared to before irradiation. There was no change in tumor size even one week after irradiation.
Academic Significance and Societal Importance of the Research Achievements	今回の結果より、レドックス代謝をイメージングバイオマーカーとする本技術は膵癌の早期診断および治療効果の早期判別に有効であると考えられる。