| Project/Area Number |
18K15384
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 52010:General internal medicine-related
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| Research Institution | University of Yamanashi |
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Principal Investigator |
OGIHARA Shinji 山梨大学, 医学部附属病院, 臨床検査技師 (80790005)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | デジタルPCR / viability-PCR / 薬剤感受性検査 / viability PCR |
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| Outline of Final Research Achievements |
In this study, to contribute to the treatment of sepsis, we constructed a method that can detect a small number of bacteria contained in a blood sample in a short time, and evaluated the usefulness of a rapid antimicrobial susceptibility test using viability- Digital PCR (dPCR). As a result, dPCR was able to detect even a very small number of bacteria of 5.6 CFU/mL. Furthermore, the direct rapid antimicrobial susceptibility test by viability-dPCR was performed on the blood of simulated sepsis patients. The direct rapid antimicrobial susceptibility test result as the conventional method was shown after 30 minutes of antimicrobial exposure.
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| Academic Significance and Societal Importance of the Research Achievements |
感染症診療は、早期診断及び適正な抗菌薬治療が重要である。特に血液中に微生物が入り込み感染症を引き起こす敗血症では、治療を間違えると死に至ることもある。現在の敗血症における検査法では、患者血液を採取して適正な抗菌薬が判明するまでに3日間はかかる。そこで、本研究では敗血症患者血液から直接微生物を見つけ出し、薬剤感受性検査を行う手法を構築した。
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