Development of blood biomarkers for Alzheimer's disease using high sensitivity digital ELISA method
| Project/Area Number |
18K15461
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 52020:Neurology-related
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| Research Institution | National Institutes for Quantum and Radiological Science and Technology (2020)
Kyoto Prefectural University of Medicine (2018-2019) |
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Principal Investigator |
Tatebe Harutsugu 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 脳機能イメージング研究部, 研究員(任常) (00637027)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 血液バイオマーカー / 認知症 / リン酸化タウ / 高感度ELISA / アルツハイマー病 / 体液バイオマーカー / ニューロフィラメント / Simoa / バイオマーカー / p-tau / tau / NFL / TDP-43 / デジタルELISA / 神経変性疾患 |
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| Outline of Final Research Achievements |
We have introduced Simoa (Single molecular array), an ultrasensitive digital assay technology, to introduce phosphorylated tau (p-tau), one of the Alzheimer's disease (AD) biomarkers, in human blood for the first time in the world. We have developed a quantitative system that can be detected on the order of fg/mL.
In addition to p-tau, we have multicenter blood and cerebrospinal fluid for other core biomarkers of AD, Aβ40, Aβ42, t-Tau (total tau protein), NF-L (neurofilament light chain). Sample measurements were taken. As a result, it was confirmed that all substances can be stably measured with Simoa.
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| Academic Significance and Societal Importance of the Research Achievements |
我々は、超高感度デジタルアッセイ技術であるSimoaを用いて、ヒトの血液中でアルツハイマー病(AD)バイオマーカーを測定する方法の開発をおこなってきた。
血液検査でAD特異的な物質を測定することに成功している。今後、認知症診断において簡便で安価な検査方法として、この血液バイオマーカーを利用できる可能性がある。
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Combined use of CSF NfL and CSF TDP-43 improves diagnostic performance in ALS2019
Author(s)
Takashi Kasai, Yuta Kojima, Takuma Ohmichi, Harutsugu Tatebe, Yukiko Tsuji, Yu-Ichi Noto, Fukiko Kitani-Morii, Makiko Shinomoto, David Allsop,Toshiki Mizuno, Takahiko Tokuda
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Journal Title
Annals of Clinical Translational Neurology
Volume: 6
Issue: 12
Pages: 2489-2502
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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