| Project/Area Number |
18K15653
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 52040:Radiological sciences-related
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2018-04-01 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | オートファジー / 細胞周期チェックポイント / 放射線治療 / 放射線増感剤 / 放射線増感効果 / 膵がん / 放射線 |
|---|
| Outline of Final Research Achievements |
A better mechanistic understanding of cellular resistance to radiotherapy should facilitate the development of novel, individualized treatment approaches. Cell-cycle checkpoint and autophagy act in concert to confer radioresistance to cells. In this study, I investigated the functional interaction between both pathways. As a result, I revealed as follows; (1) X-irradiation synchronously induced autophagy and G2 checkpoint. (2) Radiation-induced autophagy produced the ATP required for cell survival. (3) A biological crosstalk exists between G2 checkpoint and autophagy after X-irradiation, and autophagy appears to be responsible, at least in part, for G2 checkpoint-induced radioresistance.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究では核内で生じる細胞周期チェックポイントと細胞質で生じるオートファジーという異なる細胞防護機構にクロストークが存在することを明らかにした。本研究により得られた知見はこれまで明らかとなっていなかったオートファジーによる細胞防護機構の機序解明につながる。また、がん細胞の有する放射線抵抗性の機序解明に大きく寄与するものであり、今後の放射線治療増感剤の開発や治療プロトコルの作成において新たな指標となり得ることが期待される。
|
