Neo renal histology using super resolution microscopy

Research Project

Project/Area Number	18K15974
Research Category	Grant-in-Aid for Early-Career Scientists
Allocation Type	Multi-year Fund
Review Section	Basic Section 53040:Nephrology-related
Research Institution	Osaka University
Principal Investigator	Matsumoto Ayumi 大阪大学, 医学部附属病院, 医員 (40794053)
Project Period (FY)	2018-04-01 – 2020-03-31
Project Status	Completed (Fiscal Year 2019)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	腎生検 / 超解像度顕微鏡 / 腎病理
Outline of Final Research Achievements	Changes in renal microstructure tightly correlate with development and progression of kidney diseases. We found that Elastica Masson's trichrome stained kidney samples were useful to visualize podocyte foot process and tubular mitochondria using super resolution microscopy. Furthermore, we quantitatively analyze the degree of　pathological breakdown in these microstructures with fourier transform. We revealed that the measurements significantly correlated with clinical amount of proteinuria. Fourier transform could also quantify the degree of mitochondrial injury due to tubulointerstitial nephritis. Our strategy required only already in-hand samples would be useful for precision medicine based on one's detailed pathological condition.
Academic Significance and Societal Importance of the Research Achievements	本研究で我々は、一般診療に用いられているエラスチカマッソン染色などの通常染色プレパラートを、そのまま超解像度顕微鏡で観察することにより、これまで通常の光学顕微鏡で観察困難であった腎組織のポドサイト足突起やミトコンドリアが観察できることが明らかにした。また、これらの微細構造画像をフーリエ変換を用いて解析することにより、疾患による微細構造の破綻を定量的に評価することが可能であった。本方法を用いて腎生検病理を評価することでより詳細な病態を把握し、適切な治療選択に寄与できる可能性がある。