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microRNA-223 derived from platelets aggravate podocyte damage in nephrotic syndrome.

Research Project

Project/Area Number 18K15982
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 53040:Nephrology-related
Research InstitutionKagoshima University

Principal Investigator

Miyazono Akinori  鹿児島大学, 医歯学域鹿児島大学病院, 助教 (10795503)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsネフローゼ症候群 / 血小板 / microRNA / 足細胞 / miR-223 / microRNA 223 / VEGF-A
Outline of Final Research Achievements

I made a nephrotic model by administering puromycin aminonucleoside intravenously to 6-week-old SD rats. When a suspension of Exosome enriched with miR-223 derived from human megakaryoblastic leukemia cell Meg-01 was administered intravenously, urine protein/Cr was higher than that of the intravenous normal saline group.
I attempted to create a glomerular model using murine glomerular endothelial cells and podocytes. Podocytes were treated according to the product sheet. It was stated that podocytes were induced to differentiate when incubated at 38°C and stopped growing after 2 to 4 weeks, but no differentiation could be induced after 4 weeks. Protein and mRNA were extracted from podocytes and analyzed by Western-Blotting and RT-PCR, but no nephrin or synaptopodin, proteins specific to podocyte, were detected. The acquired cells themselves were thought to lack the character of a podocyte.

Academic Significance and Societal Importance of the Research Achievements

小児のネフローゼ症候群(NS)は大部分が原因不明である。難治例では長期のステロイド治療を余儀なくされ、成長障害や肥満、精神症状などの副作用に苛まれる。NSでは血小板の数や形態の異常を合併することが知られる。NSを模したラットに血小板由来のmicroRNAを注射するとNSの病勢は悪化したことからは、血小板に含有されるmicroRNAがNSの病態に関わっていることが伺えた。血小板に着目して診療することで、NSの病勢予測や治療方針の決定につながる可能性があり、究極的にはNSの発症要因にもつながると考えている。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2021-02-19  

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