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The human stomach microbiome and Helicobacter pylori infection.

Research Project

Project/Area Number 18K16182
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 54030:Infectious disease medicine-related
Research InstitutionOita University

Principal Investigator

Matsumoto Takashi  大分大学, 医学部, 助教 (50609667)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsピロリ菌感染症 / 胃マイクロバイオーム / 次世代シーケンス / 16Sメタゲノム解析 / 胃癌 / 国際共同研究 / ヘリコバクター・ピロリ / 16S rRNAメタゲノム解析 / ピロリ菌感染 / 胃マイクロバイーム / ピロリ菌 / 細菌叢
Outline of Final Research Achievements

Helicobacter pylori (H. pylori) infects with half the population and causes gastric cancer and other gastroduodenal diseases. Even though H. pylori are the strongest known risk factor for gastric cancer, less than 3% of infected patients develop gastric cancer. Thus, it remains unclear on the pathogenesis of H. pylori-related diseases, such as the regional differences in the prevalence of these diseases. To investigate the etiology of these diseases, we focused on the stomach microbiota which is symbiotic microorganisms harbored in our stomach. We aimed to understand the impact of stomach microbiota in H. pylori infection and these H. pylori-related diseases.

Academic Significance and Societal Importance of the Research Achievements

ピロリ菌の発見まで、“胃は無菌である”と長らく信じられてきた。本研究では、次世代シーケンス技術を応用した高解像度のゲノム解析を実施し、胃に生息する独自の細菌叢を同定することに成功した。また、アジア・アフリカ諸国との国際共同研究を遂行し、ピロリ菌の病原性だけでなく、胃細菌叢の構成も地域的に異なるという新たな知見を得ることができた。加えて、細菌数がごく少ない胃粘膜組織を用いた胃マイクロバイオーム解析に挑戦し、その解析技術を確立することができた。さらに、胃癌の発症には、ピロリ菌以外にも他の病原細菌が関与している可能性を明らかにし、胃癌発症機序の解明に繋がる新たな知見を広く世界に示すことができた。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (47 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (16 results) Journal Article (16 results) (of which Int'l Joint Research: 13 results,  Peer Reviewed: 16 results,  Open Access: 13 results) Presentation (12 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] MNUMS(モンゴル)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Universitas Airlangga/University of Muhammadiyah Surabaya/University of Indonesia(インドネシア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Baylor College of Medicine(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Mbujimayi/University of Kinshasa/General Referential Hospital of Bukavu(コンゴ民主共和国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Technical University of Kenya/Kenyatta University/Aga Khan Hospital University(ケニア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 国立医科学大学消化器内科(モンゴル)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] マレーシアサバ大学BMHRC/クイーンエリザベス病院内視鏡部(マレーシア)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] アガカーン大学病院病理学部/ケニア中央医学研究所(ケニア)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] キンシャサ大学/ムブジマイ大学(コンゴ民主共和国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] JDWNRH(ブータン)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] Dhaka Medical College and Hospital/BIRDEM(バングラデシュ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] Yangon General Hospital/Mandalay General Hospital/Thingangyun Sanpya General Hospital(ミャンマー)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] Thammasat University/Rajavithi Hospital/Bangkok Medical Center(タイ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] Cho Ray Hospital Ho Chi Minh/Daklak Hospital/Lao Cai Hospital(ベトナム)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research]

    • Related Report
      2018 Research-status Report
  • [Journal Article] Advantage of 16S rRNA amplicon sequencing in Helicobacter pylori diagnosis2021

    • Author(s)
      Gantuya Boldbaatar、El Serag Hashem B.、Saruuljavkhlan Batsaikhan、Azzaya Dashdorj、Matsumoto Takashi、Uchida Tomohisa、Oyuntsetseg Khasag、Oyunbileg Nyamdorj、Davaadorj Duger、Yamaoka Yoshio
    • Journal Title

      Helicobacter

      Volume: - Issue: 3

    • DOI

      10.1111/hel.12790

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers2021

    • Author(s)
      Phuc Bui Hoang、Tuan Vo Phuoc、Dung Ho Dang Quy、Binh Tran Thanh、Tung Pham Huu、Tri Tran Dinh、Thuan Ngo Phuong Minh、Van Khien Vu、Trang Tran Thi Huyen、Akada Junko、Matsumoto Takeshi、Yamaoka Yoshio
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 4584-4584

    • DOI

      10.1038/s41598-021-83862-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Discovery of unique African Helicobacter pylori CagA-multimerization motif in the Dominican Republic2020

    • Author(s)
      Ono Takaaki、Cruz Modesto、Nagashima Hiroyuki、Subsomwong Phawinee、Akada Junko、Matsumoto Takashi、Uchida Tomohisa、Suzuki Rumiko、Hosking Celso、Abreu Jos? A Jim?nez、Yamaoka Yoshio
    • Journal Title

      World Journal of Gastroenterology

      Volume: 26 Issue: 45 Pages: 7118-7130

    • DOI

      10.3748/wjg.v26.i45.7118

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Whole Genome Sequencing Reveals Virulence Potentials of Helicobacter pylori Strain KE21 Isolated from a Kenyan Patient with Gastric Signet Ring Cell Carcinoma2020

    • Author(s)
      Mwangi Catherine、Njoroge Stephen、Tshibangu-Kabamba Evariste、Moloo Zahir、Rajula Allan、Devani Smita、Matsumoto Takashi、Nyerere Kimang’a、Kariuki Samuel、Revathi Gunturu、Yamaoka Yoshio
    • Journal Title

      Toxins

      Volume: 12 Issue: 9 Pages: 556-556

    • DOI

      10.3390/toxins12090556

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Biofilm Formation and Antibiotic Resistance Phenotype of Helicobacter pylori Clinical Isolates2020

    • Author(s)
      Fauzia Kartika Afrida、Miftahussurur Muhammad、Syam Ari Fahrial、Waskito Langgeng Agung、Doohan Dalla、Rezkitha Yudith Annisa Ayu、Matsumoto Takashi、Tuan Vo Phuoc、Akada Junko、Yonezawa Hideo、Kamiya Shigeru、Yamaoka Yoshio
    • Journal Title

      Toxins

      Volume: 12 Issue: 8 Pages: 473-473

    • DOI

      10.3390/toxins12080473

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gut-Homing CD4+ T Cells Are Associated with the Activity of Gastritis in HIV-Infected Adults2020

    • Author(s)
      Sato Hidenori、Ota Yasunori、Kido Yasutoshi、Matsumoto Takashi、Matsubara Yasuo、Matano Tetsuro、Hirata Yoshihiro、Kawana-Tachikawa Ai、Yamaoka Yoshio、Yotsuyanagi Hiroshi、Adachi Eisuke
    • Journal Title

      AIDS Research and Human Retroviruses

      Volume: 36 Issue: 11 Pages: 910-917

    • DOI

      10.1089/aid.2020.0086

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High Antibiotic Resistance of Helicobacter pylori and Its Associated Novel Gene Mutations among the Mongolian Population2020

    • Author(s)
      Azzaya Dashdorj、Gantuya Boldbaatar、Oyuntsetseg Khasag、Davaadorj Duger、Matsumoto Takashi、Akada Junko、Yamaoka Yoshio
    • Journal Title

      Microorganisms

      Volume: 8 Issue: 7 Pages: 1062-1062

    • DOI

      10.3390/microorganisms8071062

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Next-Generation Sequencing of the Whole Bacterial Genome for Tracking Molecular Insight into the Broad-Spectrum Antimicrobial Resistance of Helicobacter pylori Clinical Isolates from the Democratic Republic of Congo2020

    • Author(s)
      Tshibangu-Kabamba Evariste、Ngoma-Kisoko Patrick de Jesus、Tuan Vo Phuoc、Matsumoto Takashi、Akada Junko、Kido Yasutoshi、Tshimpi-Wola Antoine、Tshiamala-Kashala Pascal、Ahuka-Mundeke Steve、Mumba Ngoy Dieudonn?、Disashi-Tumba Ghislain、Yamaoka Yoshio
    • Journal Title

      Microorganisms

      Volume: 8 Issue: 6 Pages: 887-887

    • DOI

      10.3390/microorganisms8060887

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gastric microbiota and Helicobacter pylori in Indonesian population2020

    • Author(s)
      Miftahussurur Muhammad、Waskito Langgeng Agung、El‐Serag Hashem B、Ajami Nadim J.、Nusi Iswan Abbas、Syam Ari Fahrial、Matsumoto Takashi、Rezkitha Yudith Annisa Ayu、Doohan Dalla、Fauzia Kartika Afrida、Maimunah Ummi、Sugihartono Titong、Uchida Tomohisa、Yamaoka Yoshio
    • Journal Title

      Helicobacter

      Volume: 25 Issue: 4

    • DOI

      10.1111/hel.12695

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gastric mucosal microbiota in a Mongolian population with gastric cancer and precursor conditions2020

    • Author(s)
      Gantuya Boldbaatar、El Serag Hashem B.、Matsumoto Takashi、Ajami Nadim J.、Uchida Tomohisa、Oyuntsetseg Khasag、Bolor Dashdorj、Yamaoka Yoshio
    • Journal Title

      Alimentary Pharmacology & Therapeutics

      Volume: 51 Issue: 8 Pages: 770-780

    • DOI

      10.1111/apt.15675

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Long-Term Potable Effects of Alkalescent Mineral Water on Intestinal Microbiota Shift and Physical Conditioning2019

    • Author(s)
      Yahiro Takaaki、Hara Takao、Matsumoto Takashi、Ikebe Emi、Fife-Koshinomi Nichole、Xu Zhaojun、Hiratsuka Takahiro、Iha Hidekatsu、Inomata Masafumi
    • Journal Title

      Evidence-Based Complementary and Alternative Medicine

      Volume: 2019 Pages: 1-10

    • DOI

      10.1155/2019/2710587

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome-wide mutation analysis of Helicobacter pylori after inoculation to Mongolian gerbils2019

    • Author(s)
      Suzuki Rumiko、Satou Kazuhito、Shiroma Akino、Shimoji Makiko、Teruya Kuniko、Matsumoto Takashi、Akada Junko、Hirano Takashi、Yamaoka Yoshio
    • Journal Title

      Gut Pathogens

      Volume: 11 Issue: 1 Pages: 1-6

    • DOI

      10.1186/s13099-019-0326-5

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel bat-associated circovirus identified in northern Hokkaido, Japan.2019

    • Author(s)
      Matsumoto T, Sato M, Nishizono A, Ahmed K.
    • Journal Title

      Arch Virol.

      Volume: 164(8) Issue: 8 Pages: 2179-2182

    • DOI

      10.1007/s00705-019-04286-x

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Next-Generation Sequencing-Based Approach to Identify Genetic Determinants of Antibiotic Resistance in Cambodian Helicobacter pylori Clinical Isolates2019

    • Author(s)
      Tuan Vo Phuoc、Narith Dou、Tshibangu-Kabamba Evariste、Dung Ho Dang Quy、Viet Pham Thanh、Sokomoth Sin、Binh Tran Thanh、Sokhem Sok、Tri Tran Dinh、Ngov Seng、Tung Pham Huu、Thuan Ngo Phuong Minh、Truc Tran Cong、Phuc Bui Hoang、Matsumoto Takashi、Fauzia Kartika Afrida、Akada Junko、Trang Tran Thi Huyen、Yamaoka Yoshio
    • Journal Title

      Journal of Clinical Medicine

      Volume: 8 Issue: 6 Pages: 858-858

    • DOI

      10.3390/jcm8060858

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gastric Microbiota in Helicobacter pylori-Negative and -Positive Gastritis Among High Incidence of Gastric Cancer Area2019

    • Author(s)
      Gantuya Boldbaatar、El-Serag Hashem B.、Matsumoto Takashi、Ajami Nadim J.、Oyuntsetseg Khasag、Azzaya Dashdorj、Uchida Tomohisa、Yamaoka Yoshio
    • Journal Title

      Cancers

      Volume: 11 Issue: 4 Pages: 504-504

    • DOI

      10.3390/cancers11040504

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dysbiosis of the Gut Microbiota on the Inflammatory Background due to Lack of Suppressor of Cytokine Signalling-1 in Mice2018

    • Author(s)
      Gendo Y、Matsumoto T、Kamiyama N、Saechue B、Fukuda C、Dewayani A、Hidano S、Noguchi K、Sonoda A、Ozaki T、Sachi N、Hirose H、Ozaka S、Eshita Y、Mizukami K、Okimoto T、Kodama M、Yoshimatsu T、Nishida H、Daa T、Yamaoka Y、Murakami K、Kobayashi T
    • Journal Title

      Inflammatory Intestinal Diseases

      Volume: 3 Issue: 3 Pages: 145-154

    • DOI

      10.1159/000495462

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Gastric Microbiota: From Sampling to Obtain the Sequence2021

    • Author(s)
      Takashi Matsumoto
    • Organizer
      International workshop Gastroenterology Research Opportunities in Antimicrobial Resistance, Metagenomic, Clinical Surveillance and Wet-Lab Experiment at ITD, UNAIR
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Helicobacter pylori infection and gastric microbiota2020

    • Author(s)
      Takashi Matsumoto, Yoshio Yamaoka
    • Organizer
      Aga Khan university Symposium on H. pylori infection
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] The human gastric microbiome and Helicobacter pylori infection2020

    • Author(s)
      Takashi Matsumoto
    • Organizer
      Mongolian Digestive Disease Week 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Helicobacter pylori infection and gastric microbiota2020

    • Author(s)
      Takashi Matsumoto
    • Organizer
      KEMRI Annual Scientific and Health Conference
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Helicobacter pylori infection and gastric microbiota2020

    • Author(s)
      Takashi Matsumoto
    • Organizer
      10th Kenya Medical Research Institute Annual Scientific and Health Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mechanistic insights of metronidazole resistance revealed by next-generation sequencing of Helicobacter pylori from Democratic Republic of Congo2019

    • Author(s)
      Tshibangu Evariste, Tuan VP, Patrick Ngoma, Pascal Tshiamala, Antoine Tshimpi, Matsumoto T, Akada J, Kido Y, Ahuka-Mundeke Steve, Mumba-Ngoyi Dieudonne, Dishashi Tumba, Yamaoka Y.
    • Organizer
      The 10th International Congress on Infectious Diseases
    • Related Report
      2019 Research-status Report
  • [Presentation] ゲノムワイド関連解析を用いたピロリ菌病原因子の検索2019

    • Author(s)
      松本昂、山岡吉生
    • Organizer
      第51回九州微生物学研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] Techniques in 16S Metagenomics and Stomach Microbiome.2019

    • Author(s)
      Takashi Matsumoto
    • Organizer
      At Universiti Malaysia Sabah (Malaysia) as Guest Speaker
    • Related Report
      2018 Research-status Report
  • [Presentation] ヘリコバクター・ピロリ感染による胃細菌叢の変化2018

    • Author(s)
      松本昂、山岡吉生
    • Organizer
      第71回日本細菌学会九州支部総会・第55 回日本ウイルス学会九州支部総会
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 胃マイクロバイームに関する検討2018

    • Author(s)
      山岡吉生、松本昂
    • Organizer
      第50回九州微生物研究会総会
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Microbiome in the stomach and Helicobacter pylori infection in Asian population.2018

    • Author(s)
      Takashi Matsumoto
    • Organizer
      Seminar on the Formation of gastrointestinal infectious disease center mainly focused on Helicobacter pylori infection in African countries. (JSPS Core-to-Core program) at KEMRI, Kenya.
    • Related Report
      2018 Research-status Report
  • [Presentation] Microbiome in the stomach and Helicobacter pylori infection in Asian population.2018

    • Author(s)
      Takashi Matsumoto
    • Organizer
      Seminar on the Formation of gastrointestinal infectious disease center mainly focused on Helicobacter pylori infection in African countries. (JSPS Core-to-Core program) at Aga Khan University, Kenya.
    • Related Report
      2018 Research-status Report
  • [Book] Microbiota: Current Research and Emerging Trends2019

    • Author(s)
      Takashi Matsumoto and Yoshio Yamaoka
    • Total Pages
      126
    • Publisher
      Caister Academic Press
    • ISBN
      9781910190944
    • Related Report
      2018 Research-status Report
  • [Remarks] 大分大学医学部環境・予防医学講座

    • URL

      http://phealth2.wp.med.oita-u.ac.jp/

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
  • [Remarks] 大分大学医学部環境・予防医学講座

    • URL

      http://www.med.oita-u.ac.jp/phealth2/http://www.med.oita-u.ac.jp/phealth2/

    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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