The function and identification of the specific proteins of multidrug-resistant Escherichia coli ST131
Project/Area Number |
18K16189
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 54030:Infectious disease medicine-related
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Research Institution | Tenri Health Care University |
Principal Investigator |
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | Escherichia coli / sequence type 131 / YahO / 可溶性チトクロームb562 / HdeA / YjbJ / ESBL / carbapenemase / proteomics / Escherichia coli ST131 / specific proteins / YnfD / cytochrome b562 |
Outline of Final Research Achievements |
Escherichia coli ST131-specific proteins were detected, including YahO protein with E34A amino acid substitution with 7655 m/z (unknown function), soluble cytochrome b562 with 11783 m/z (unknown function), HdeA with 9710 m/z (acid stress response related), and YjbJ protein with 8351 m/z (unknown function). In addition, we found a sequence unique to ST131 C clade in soluble cytochrome b562 with 11783 m/z. The predicted protein-protein interactions of YahO and soluble cytochrome b562, whose functions are currently unknown, suggest that they may be related in biofilm formation.
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Academic Significance and Societal Importance of the Research Achievements |
ESBLやカルバペネマーゼを産生するEscherichia coli ST131の世界的なパンデミックが問題視されているが、その原因は未だ明らかになっていない。われわれはST131パンデミックの原因を追究すべく、MALDI-TOF MSを用いてST131特異的タンパク質を探索した。その結果主に4つのアミノ酸置換をともなうタンパク質を発見した。しかし、これらの中にはその機能が現在のところ不明なものが多く存在する。今後、これらの機能を明らかにすることにより、薬剤耐性菌が世界的パンデミックを引き起こす原因が追究できる可能性を秘めている。
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Evaluation of the modified carbapenem inactivation method for the detection of carbapenemase-producing Enterobacteriaceae.2018
Author(s)
Kuchibiro T, Komatsu M, Yamasaki K, Nakamura T, Nishio H, Nishi I, Kimura K, Niki M, Ono T, Sueyoshi N, Kita M, Kida K, Ohama M, Satoh K, Toda H, Mizutani T, Fukuda N, Sawa K, Nakai I, Kofuku T, Orita T, Watari H, Shimura S, Fukuda S, Nakamura A, Wada Y.
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Journal Title
J Infect Chemother
Volume: 24
Issue: 4
Pages: 262-266
DOI
Related Report
Peer Reviewed
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