Investigation for intestinal microbiome and gut hormone of short bowel syndrome

• Project/Area Number: 18K16262
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55010:General surgery and pediatric surgery-related
• Research Institution: Kagoshima University
• Principal Investigator: Onishi Shun 鹿児島大学, 鹿児島大学病院, 特任助教 (10614638)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 短腸症候群 / 消化管ホルモン / 腸内フローラ / 脂肪酸 / 小児外科 / IFALD / 腸管不全合併肝障害 / グレリン / GLP-2 / (IFALD

Outline of Final Research Achievements

Intestinal failure including short bowel syndrome is one of the challenging situation in pediatric surgery field. Intestinal failure associated liver disease (IFALD) is the critical complication. Development of total parenteral nutrition and operative management improve the survival rate, however, the mechanism of IFALD is not unveiled. We evaluated the mechanism of IFALD and effect of administration of omega-3 fatty acid. The main results of this research are as followed; 1) administration of omega-3 fatty acid accelerate intestinal adaptation; 2) administration of omega-3 fatty acid prevent IFALD.

Academic Significance and Societal Importance of the Research Achievements

本研究の内容は成人において長期絶食を余儀なくされた患者におけるPNALDや腸管絨毛の委縮の予防、上腸間膜動脈症候群や外傷によって短腸症候群となった患者についても応用が可能であり、対象となる患者数は潜在的に非常に多いと考える。
小児・成人の幅広い分野において管理に難渋する疾患である大量腸管切除後の腸管順応およびIFALDの予防における新たな術後管理の確立に向けた第一歩であり、社会的な意義は大きいと考える。

Research Products

• [Journal Article] What is the optimal lipid emulsion for preventing intestinal failure-associated liver disease following parenteral feeding in a rat model of short-bowel syndrome? (2021)
  • Author(s): Machigashira Seiro、Kaji Tatsuru、Onishi Shun、Yano Keisuke、Harumatsu Toshio、Yamada Koji、Yamada Waka、Matsukubo Makoto、Muto Mitsuru、Ieiri Satoshi
  • Journal Title: Pediatric Surgery International Volume: 37 Issue: 2 Pages: 247-256
  • DOI: 10.1007/s00383-020-04802-0
  • Peer Reviewed / Open Access
• [Journal Article] An overview of the current management of short-bowel syndrome in pediatric patients (2021)
  • Author(s): Muto Mitsuru、Kaji Tatsuru、Onishi Shun、Yano Keisuke、Yamada Waka、Ieiri Satoshi
  • Journal Title: Surgery Today Volume: ー Issue: 1 Pages: 12-21
  • DOI: 10.1007/s00595-020-02207-z
  • Peer Reviewed / Open Access
• [Journal Article] A Comparison of the Effectiveness Between Three Different Endoscopic Surgical Skill Training Programs for Medical Students Using the Infant Laparoscopic Fundoplication Simulator: A Randomized Controlled Trial (2019)
  • Author(s): Onishi Shun、Ikee Takamasa、Murakami Masakazu、Yano Keisuke、Harumatsu Toshio、Baba Tokuro、Yamada Koji、Yamada Waka、Masuya Ryuta、Machigashira Seiro、Nakame Kazuhiko、Mukai Motoi、Kaji Tatsuru、Ieiri Satoshi
  • Journal Title: Journal of Laparoendoscopic & Advanced Surgical Techniques Volume: 29 Issue: 10 Pages: 1252-1258
  • DOI: 10.1089/lap.2019.0212
  • Peer Reviewed / Open Access
• [Journal Article] Novel effect of glucagon-like peptide-2 for hepatocellular injury in a parenterally fed rat model of short bowel syndrome. (2019)
  • Author(s): Yano K, Kaji T, Onishi S, Machigashira S, Nagai T, Harumatsu T, Yamada K, Yamada W, Muto M, Nakame K, Mukai M, Ieiri S.
  • Journal Title: Pediatric Surgery International Volume: 35 Issue: 12 Pages: 1345-1351
  • DOI: 10.1007/s00383-019-04560-8
  • Peer Reviewed / Open Access
• [Journal Article] Partially hydrolyzed guar gum alleviates small intestinal mucosal damage after massive small bowel resection along with changes in the intestinal microbiota (2019)
  • Author(s): Fujii Takayuki、Chiba Yoichi、Nakayama-Imaohji Haruyuki、Onishi Shun、Tanaka Aya、Katami Hiroto、Kaji Tatsuru、Ieiri Satoshi、Miki Takanori、Ueno Masaki、Kuwahara Tomomi、Shimono Ryuichi
  • Journal Title: Journal of Pediatric Surgery Volume: 54 Issue: 12 Pages: 2514-2519
  • DOI: 10.1016/j.jpedsurg.2019.08.048
  • Peer Reviewed / Open Access
• [Journal Article] Ghrelin Stimulates Intestinal Adaptation Following Massive Small Bowel Resection (2018)
  • Author(s): Onishi S, Kaji T, Yamada W, Nakame K, Machigashira S, Yano K, Kawano M, Harumatsu T,Masuya R, Kawano T, Mukai M, Yoshioka T, Souda M, Hamada T, Tanimoto A, Ieiri S
  • Journal Title: Peptide Volume: 106 Pages: 264-267
  • DOI: 10.1016/j.peptides.2018.06.009
  • Peer Reviewed / Open Access
• [Journal Article] The effect of intravenous lipid emulsions and mucosal adaptation following massive bowel resection (2018)
  • Author(s): Onishi Shun、Kaji Tatsuru、Machigashira Seiro、Yamada Waka、Masuya Ryuta、Nakame Kazuhiko、Kawano Masato、Yano Keisuke、Yamada Koji、Kawano Takafumi、Mukai Motoi、Yoshioka Takako、Tanimoto Akihide、Ieiri Satoshi
  • Journal Title: Journal of Pediatric Surgery Volume: 53 Issue: 12 Pages: 2444-2448
  • DOI: 10.1016/j.jpedsurg.2018.08.019
  • Peer Reviewed / Open Access
• [Presentation] The impact of morphological affect for the liver and the small bowel induced by massive bowel resection in rat model (2019)
  • Author(s): Onishi S, Kaji T, Machigashira S, Yano K, Harumatsu T, Baba T, Yamada K, Yamada W, Masuya R, Nakame K, Ieiri S.
  • Organizer: 32nd International Symposium on Pediatric Surgical Research
  • Int'l Joint Research
• [Presentation] The efficiency and mechanism of ω3 fatty acid on improving intestinal failure-associated liver disease in a rat model of short bowel syndrome (2019)
  • Author(s): Machigashira S, Kaji T, Onishi S, Murakami M, Yano K, Baba T, Harumatsu T, Yamada K, Yamada W, Masuya R, Kawano T, Nakame K, Mukai M, Ieiri S
  • Organizer: The 52nd Annual Meeting of the Pacific Association of Pediatric Surgeons
  • Int'l Joint Research
• [Presentation] The novel effect of fish oil emulsions for intestinal mucosal adaptation following massive bowel resection (2018)
  • Author(s): Onishi S, Kaji T, Yamada Y, Moriguchi T, Sugita K, Yamada K, Kawano T, Nakame K, Mukai M, Yamada S, Yoshioka T, Tanimoto A, Ieiri S
  • Organizer: Pacific Association of Pediatric Surgeons 51th Annual Conference
• [Presentation] Strategy of Neonatal Airway Obstruction with Team Medical Care (2018)
  • Author(s): Onishi S, Nakame K, Yano K, Harumatsu T, Yamada K, Yamada W, Masuya R, Kawano T, Machigashira S, Mukai M, Kaji T, Ieiri S
  • Organizer: The Federation of Asia and Oceania Perinatal Societies