Project/Area Number |
18K16402
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 55030:Cardiovascular surgery-related
|
Research Institution | Keio University |
Principal Investigator |
|
Project Period (FY) |
2018-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 虚血性脊髄障害 / 脊髄虚血 / 大動脈瘤 / 大動脈解離 / 肝細胞増殖因子 / 胸部大動脈手術 / 大動脈 |
Outline of Final Research Achievements |
In this study, I performed ELISA and realtime PCR using spinal cord ischemia rats. The levels of HGF in spinal cord and serum were calculated with ELISA. The levels of HGF in SCI rat spinal cord L1-2 in SCI rats were higher than that of the control group. The levels of c-Met mRNA in spinal cord Th10-13 were elevated just after injury. These findings are similar to those of traumatic spinal cord injury.Then we infused recombinant HGF intrathecally into SCI rats. Two SCI rats were recovered from paraplegia in HGF group(n=3), however in PBS group(n=5), there was no fuctional recovery of paraplegia. In conclusion, HGF treatment for SCI rats may be effective but there are limitations. The first and most important limitation is number of rats. We performed only 8 rats(HGF group; n=3, PBS group; n=5). we have to continue this study. The second limitation is this study was performed with small animal, rat. After this study, we start next study using large animal and human.
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Academic Significance and Societal Importance of the Research Achievements |
大動脈疾患は近年増加傾向にある。その合併症である虚血性脊髄障害(対麻痺、下半身麻痺)は、様々な予防法が開発され、施行されている現在においても完全には予防できず、その治療法も無い。本研究では外傷性脊髄損傷において有効性の示唆されている肝細胞増殖因子(HGF)に着目した。虚血性脊髄障害に発症後にHGFを投与することで下肢機能の回復を得ることが本研究の最大の目的である。今回の研究にて、HGF投与の有効性が示唆された。さらなる研究が必要とされるjyきょうではあるが、大動脈手術後の合併症(対麻痺)に悩む患者に対する治療の一つとなることが示唆される研究となった。
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